Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0075 and Psyr_0076 are separated by 51 nucleotides Psyr_0076 and Psyr_0077 are separated by 591 nucleotides
Psyr_0075: Psyr_0075 - ATP-dependent DNA helicase Rep, at 79,502 to 81,511
_0075
Psyr_0076: Psyr_0076 - Xanthine phosphoribosyltransferase, at 81,563 to 82,132
_0076
Psyr_0077: Psyr_0077 - sigma54 specific transcriptional regulator, Fis family, at 82,724 to 84,043
_0077
Position (kb)
81
82
83 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 81.521 kb on + strand at 81.532 kb on + strand at 81.532 kb on + strand at 81.532 kb on + strand at 81.533 kb on - strand at 81.560 kb on + strand at 81.561 kb on - strand at 81.561 kb on - strand at 81.561 kb on - strand at 81.561 kb on - strand at 81.561 kb on - strand at 81.561 kb on - strand at 81.603 kb on + strand at 81.603 kb on + strand at 81.603 kb on + strand at 81.603 kb on + strand at 81.604 kb on - strand at 81.604 kb on - strand at 81.604 kb on - strand at 81.851 kb on + strand, within Psyr_0076 at 81.851 kb on + strand, within Psyr_0076 at 81.851 kb on + strand, within Psyr_0076 at 81.852 kb on - strand, within Psyr_0076 at 81.852 kb on - strand, within Psyr_0076 at 81.852 kb on - strand, within Psyr_0076 at 82.130 kb on + strand at 82.130 kb on + strand at 82.130 kb on + strand at 82.130 kb on + strand at 82.130 kb on + strand at 82.131 kb on - strand at 82.131 kb on - strand at 82.131 kb on - strand at 82.131 kb on - strand at 82.192 kb on + strand at 82.193 kb on - strand at 82.193 kb on - strand at 82.193 kb on - strand at 82.421 kb on + strand at 82.421 kb on + strand at 82.425 kb on + strand at 82.425 kb on + strand at 82.425 kb on + strand at 82.425 kb on + strand at 82.425 kb on + strand at 82.425 kb on + strand at 82.426 kb on - strand at 82.426 kb on - strand at 82.426 kb on - strand at 82.426 kb on - strand at 82.426 kb on - strand at 82.535 kb on + strand at 82.649 kb on + strand at 82.649 kb on + strand at 82.649 kb on + strand at 82.711 kb on + strand at 82.867 kb on - strand, within Psyr_0077 at 82.898 kb on + strand, within Psyr_0077 at 82.942 kb on + strand, within Psyr_0077 at 82.946 kb on + strand, within Psyr_0077 at 82.947 kb on - strand, within Psyr_0077 at 82.947 kb on - strand, within Psyr_0077 at 82.947 kb on - strand, within Psyr_0077 at 82.947 kb on - strand, within Psyr_0077 at 82.964 kb on + strand, within Psyr_0077 at 82.964 kb on + strand, within Psyr_0077 at 82.964 kb on + strand, within Psyr_0077 at 82.965 kb on - strand, within Psyr_0077 at 82.965 kb on - strand, within Psyr_0077 at 82.965 kb on - strand, within Psyr_0077 at 82.965 kb on - strand, within Psyr_0077 at 82.965 kb on - strand, within Psyr_0077 at 82.965 kb on - strand, within Psyr_0077 at 82.965 kb on - strand, within Psyr_0077 at 82.965 kb on - strand, within Psyr_0077 at 83.001 kb on + strand, within Psyr_0077 at 83.001 kb on + strand, within Psyr_0077 at 83.002 kb on - strand, within Psyr_0077 at 83.002 kb on - strand, within Psyr_0077
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 81,521 + -0.8 81,532 + +3.0 81,532 + -0.1 81,532 + +3.0 81,533 - +1.0 81,560 + +4.5 81,561 - -3.0 81,561 - -1.7 81,561 - +0.4 81,561 - -0.0 81,561 - +3.2 81,561 - -2.4 81,603 + -2.2 81,603 + -2.7 81,603 + -1.9 81,603 + +3.4 81,604 - +0.2 81,604 - -0.9 81,604 - +0.2 81,851 + Psyr_0076 0.51 -0.7 81,851 + Psyr_0076 0.51 -0.5 81,851 + Psyr_0076 0.51 +1.0 81,852 - Psyr_0076 0.51 +4.1 81,852 - Psyr_0076 0.51 -1.2 81,852 - Psyr_0076 0.51 +1.5 82,130 + -4.6 82,130 + -0.3 82,130 + -1.3 82,130 + +1.8 82,130 + -0.8 82,131 - -1.9 82,131 - -2.4 82,131 - +0.5 82,131 - -0.3 82,192 + -1.1 82,193 - +2.5 82,193 - -1.7 82,193 - +1.6 82,421 + -0.9 82,421 + +0.2 82,425 + +0.3 82,425 + +2.5 82,425 + +0.5 82,425 + -0.3 82,425 + -0.9 82,425 + -0.1 82,426 - +0.9 82,426 - -1.5 82,426 - +2.8 82,426 - +1.8 82,426 - +2.8 82,535 + +0.6 82,649 + -0.5 82,649 + +0.5 82,649 + +1.1 82,711 + +3.7 82,867 - Psyr_0077 0.11 +0.5 82,898 + Psyr_0077 0.13 -1.1 82,942 + Psyr_0077 0.17 +1.0 82,946 + Psyr_0077 0.17 +2.2 82,947 - Psyr_0077 0.17 -0.5 82,947 - Psyr_0077 0.17 -1.2 82,947 - Psyr_0077 0.17 -0.8 82,947 - Psyr_0077 0.17 +3.4 82,964 + Psyr_0077 0.18 +3.8 82,964 + Psyr_0077 0.18 -1.0 82,964 + Psyr_0077 0.18 -1.9 82,965 - Psyr_0077 0.18 +1.8 82,965 - Psyr_0077 0.18 -0.4 82,965 - Psyr_0077 0.18 +0.2 82,965 - Psyr_0077 0.18 +1.5 82,965 - Psyr_0077 0.18 -1.4 82,965 - Psyr_0077 0.18 -0.2 82,965 - Psyr_0077 0.18 +1.8 82,965 - Psyr_0077 0.18 +0.1 83,001 + Psyr_0077 0.21 -0.9 83,001 + Psyr_0077 0.21 +0.1 83,002 - Psyr_0077 0.21 -0.9 83,002 - Psyr_0077 0.21 +3.7
Or see this region's nucleotide sequence