Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0036 and Psyr_0037 are separated by 165 nucleotides Psyr_0037 and Psyr_0038 are separated by 150 nucleotides Psyr_0038 and Psyr_0039 are separated by 137 nucleotides Psyr_0039 and Psyr_0040 are separated by 212 nucleotides Psyr_0040 and Psyr_0041 are separated by 118 nucleotides Psyr_0041 and Psyr_0042 are separated by 0 nucleotides
Psyr_0036: Psyr_0036 - protein of unknown function DUF883, at 40,264 to 40,590
_0036
Psyr_0037: Psyr_0037 - Protein of unknown function DUF1458, at 40,756 to 40,971
_0037
Psyr_0038: Psyr_0038 - Protein of unknown function DUF1161, at 41,122 to 41,346
_0038
Psyr_0039: Psyr_0039 - luciferase, at 41,484 to 42,485
_0039
Psyr_0040: Psyr_0040 - OsmC-like protein, at 42,698 to 43,129
_0040
Psyr_0041: Psyr_0041 - Protein of unknown function DUF1161, at 43,248 to 43,481
_0041
Psyr_0042: Psyr_0042 - lipoprotein, putative, at 43,482 to 43,970
_0042
Position (kb) 41
42
43 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 40.552 kb on - strand, within Psyr_0036 at 40.552 kb on - strand, within Psyr_0036 at 40.581 kb on + strand at 40.581 kb on + strand at 40.581 kb on + strand at 40.581 kb on + strand at 40.581 kb on + strand at 40.581 kb on + strand at 40.581 kb on + strand at 40.581 kb on + strand at 40.582 kb on - strand at 40.582 kb on - strand at 40.582 kb on - strand at 40.582 kb on - strand at 40.582 kb on - strand at 40.582 kb on - strand at 40.582 kb on - strand at 40.582 kb on - strand at 40.582 kb on - strand at 40.584 kb on + strand at 40.584 kb on + strand at 40.720 kb on + strand at 40.720 kb on + strand at 40.720 kb on + strand at 40.720 kb on + strand at 40.721 kb on - strand at 40.721 kb on - strand at 40.721 kb on - strand at 40.721 kb on - strand at 40.722 kb on + strand at 40.774 kb on + strand at 40.774 kb on + strand at 40.774 kb on + strand at 40.774 kb on + strand at 40.774 kb on + strand at 40.774 kb on + strand at 40.774 kb on + strand at 40.775 kb on - strand at 40.775 kb on - strand at 40.775 kb on - strand at 40.934 kb on + strand, within Psyr_0037 at 40.934 kb on + strand, within Psyr_0037 at 40.935 kb on - strand, within Psyr_0037 at 40.969 kb on + strand at 40.969 kb on + strand at 40.970 kb on - strand at 40.970 kb on - strand at 41.006 kb on + strand at 41.016 kb on + strand at 41.017 kb on - strand at 41.017 kb on - strand at 41.030 kb on + strand at 41.030 kb on + strand at 41.144 kb on + strand at 41.248 kb on + strand, within Psyr_0038 at 41.248 kb on + strand, within Psyr_0038 at 41.248 kb on + strand, within Psyr_0038 at 41.248 kb on + strand, within Psyr_0038 at 41.248 kb on + strand, within Psyr_0038 at 41.248 kb on + strand, within Psyr_0038 at 41.249 kb on - strand, within Psyr_0038 at 41.249 kb on - strand, within Psyr_0038 at 41.515 kb on + strand at 41.542 kb on + strand at 41.542 kb on + strand at 41.542 kb on + strand at 41.542 kb on + strand at 41.542 kb on + strand at 41.542 kb on + strand at 41.542 kb on + strand at 41.542 kb on + strand at 41.542 kb on + strand at 41.543 kb on - strand at 41.543 kb on - strand at 41.543 kb on - strand at 41.543 kb on - strand at 41.543 kb on - strand at 41.543 kb on - strand at 41.543 kb on - strand at 41.543 kb on - strand at 41.543 kb on - strand at 41.543 kb on - strand at 41.543 kb on - strand at 41.543 kb on - strand at 41.543 kb on - strand at 41.543 kb on - strand at 41.543 kb on - strand at 41.555 kb on + strand at 41.655 kb on + strand, within Psyr_0039 at 41.756 kb on - strand, within Psyr_0039 at 41.774 kb on - strand, within Psyr_0039 at 41.774 kb on - strand, within Psyr_0039 at 41.774 kb on - strand, within Psyr_0039 at 41.827 kb on + strand, within Psyr_0039 at 41.828 kb on - strand, within Psyr_0039 at 41.828 kb on - strand, within Psyr_0039 at 41.828 kb on - strand, within Psyr_0039 at 41.869 kb on + strand, within Psyr_0039 at 41.893 kb on + strand, within Psyr_0039 at 41.894 kb on - strand, within Psyr_0039 at 41.894 kb on - strand, within Psyr_0039 at 42.049 kb on + strand, within Psyr_0039 at 42.049 kb on + strand, within Psyr_0039 at 42.049 kb on + strand, within Psyr_0039 at 42.050 kb on - strand, within Psyr_0039 at 42.659 kb on + strand at 42.660 kb on - strand at 42.798 kb on - strand, within Psyr_0040 at 42.798 kb on - strand, within Psyr_0040 at 42.935 kb on - strand, within Psyr_0040 at 42.963 kb on + strand, within Psyr_0040 at 42.963 kb on + strand, within Psyr_0040 at 42.963 kb on + strand, within Psyr_0040 at 42.963 kb on + strand, within Psyr_0040 at 42.963 kb on + strand, within Psyr_0040 at 42.963 kb on + strand, within Psyr_0040 at 42.963 kb on + strand, within Psyr_0040 at 42.964 kb on - strand, within Psyr_0040 at 42.964 kb on - strand, within Psyr_0040 at 42.964 kb on - strand, within Psyr_0040 at 43.114 kb on + strand at 43.114 kb on + strand at 43.114 kb on + strand at 43.114 kb on + strand at 43.115 kb on - strand at 43.115 kb on - strand at 43.115 kb on - strand at 43.115 kb on - strand at 43.115 kb on - strand at 43.115 kb on - strand at 43.169 kb on + strand at 43.169 kb on + strand at 43.170 kb on - strand at 43.170 kb on - strand at 43.178 kb on + strand at 43.178 kb on + strand at 43.178 kb on + strand at 43.178 kb on + strand at 43.178 kb on + strand at 43.178 kb on + strand at 43.178 kb on + strand at 43.179 kb on - strand at 43.179 kb on - strand at 43.179 kb on - strand at 43.180 kb on + strand at 43.180 kb on + strand at 43.180 kb on + strand at 43.180 kb on + strand at 43.181 kb on - strand at 43.181 kb on - strand at 43.181 kb on - strand at 43.230 kb on + strand at 43.230 kb on + strand at 43.260 kb on - strand at 43.374 kb on + strand, within Psyr_0041 at 43.374 kb on + strand, within Psyr_0041 at 43.375 kb on - strand, within Psyr_0041 at 43.394 kb on + strand, within Psyr_0041 at 43.461 kb on + strand at 43.461 kb on + strand at 43.461 kb on + strand at 43.461 kb on + strand at 43.461 kb on + strand at 43.461 kb on + strand at 43.461 kb on + strand at 43.461 kb on + strand at 43.461 kb on + strand at 43.461 kb on + strand at 43.462 kb on - strand at 43.462 kb on - strand at 43.462 kb on - strand at 43.462 kb on - strand at 43.462 kb on - strand at 43.462 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 40,552 - Psyr_0036 0.88 -1.0 40,552 - Psyr_0036 0.88 +0.2 40,581 + -0.6 40,581 + +0.3 40,581 + +0.3 40,581 + -0.1 40,581 + -0.3 40,581 + -1.2 40,581 + +1.5 40,581 + +3.5 40,582 - +0.7 40,582 - -2.8 40,582 - +3.5 40,582 - +0.9 40,582 - +4.2 40,582 - +2.1 40,582 - -1.1 40,582 - +0.2 40,582 - +2.5 40,584 + +4.5 40,584 + +2.2 40,720 + -0.6 40,720 + -0.5 40,720 + -1.0 40,720 + -2.5 40,721 - +3.8 40,721 - +1.2 40,721 - +3.7 40,721 - -1.2 40,722 + -0.7 40,774 + -1.5 40,774 + -0.8 40,774 + -0.6 40,774 + -0.7 40,774 + -1.1 40,774 + +0.1 40,774 + -0.1 40,775 - +3.5 40,775 - -2.2 40,775 - +0.3 40,934 + Psyr_0037 0.82 +2.5 40,934 + Psyr_0037 0.82 +0.7 40,935 - Psyr_0037 0.83 +0.5 40,969 + -1.9 40,969 + -3.8 40,970 - +1.0 40,970 - -0.9 41,006 + +0.9 41,016 + -1.1 41,017 - -0.6 41,017 - -0.3 41,030 + -0.6 41,030 + +0.6 41,144 + -0.5 41,248 + Psyr_0038 0.56 +4.5 41,248 + Psyr_0038 0.56 +1.8 41,248 + Psyr_0038 0.56 +0.9 41,248 + Psyr_0038 0.56 -0.4 41,248 + Psyr_0038 0.56 -1.8 41,248 + Psyr_0038 0.56 -0.8 41,249 - Psyr_0038 0.56 -1.4 41,249 - Psyr_0038 0.56 -1.8 41,515 + +0.4 41,542 + -1.5 41,542 + -1.5 41,542 + -0.9 41,542 + +0.8 41,542 + +0.2 41,542 + -0.4 41,542 + +2.2 41,542 + -1.0 41,542 + -0.1 41,543 - +4.9 41,543 - +0.9 41,543 - -0.2 41,543 - +1.8 41,543 - -0.6 41,543 - +0.3 41,543 - -0.6 41,543 - -1.6 41,543 - +0.3 41,543 - +1.3 41,543 - -2.2 41,543 - +1.2 41,543 - +3.9 41,543 - +3.4 41,543 - -0.4 41,555 + -1.5 41,655 + Psyr_0039 0.17 +2.5 41,756 - Psyr_0039 0.27 -2.1 41,774 - Psyr_0039 0.29 +0.2 41,774 - Psyr_0039 0.29 -0.4 41,774 - Psyr_0039 0.29 +2.2 41,827 + Psyr_0039 0.34 +1.5 41,828 - Psyr_0039 0.34 +0.5 41,828 - Psyr_0039 0.34 -0.5 41,828 - Psyr_0039 0.34 +3.0 41,869 + Psyr_0039 0.38 -1.3 41,893 + Psyr_0039 0.41 +0.3 41,894 - Psyr_0039 0.41 -1.8 41,894 - Psyr_0039 0.41 -2.2 42,049 + Psyr_0039 0.56 +3.1 42,049 + Psyr_0039 0.56 +2.1 42,049 + Psyr_0039 0.56 +2.2 42,050 - Psyr_0039 0.56 +0.1 42,659 + -2.6 42,660 - -1.0 42,798 - Psyr_0040 0.23 +4.3 42,798 - Psyr_0040 0.23 -0.6 42,935 - Psyr_0040 0.55 +0.7 42,963 + Psyr_0040 0.61 +1.1 42,963 + Psyr_0040 0.61 +3.0 42,963 + Psyr_0040 0.61 +0.2 42,963 + Psyr_0040 0.61 +0.6 42,963 + Psyr_0040 0.61 -0.7 42,963 + Psyr_0040 0.61 +3.0 42,963 + Psyr_0040 0.61 -1.1 42,964 - Psyr_0040 0.62 -1.3 42,964 - Psyr_0040 0.62 -1.9 42,964 - Psyr_0040 0.62 +2.2 43,114 + -3.7 43,114 + -1.3 43,114 + -1.8 43,114 + -1.1 43,115 - +4.7 43,115 - +0.2 43,115 - +3.4 43,115 - +4.1 43,115 - +0.8 43,115 - +2.5 43,169 + -2.5 43,169 + +2.5 43,170 - +1.8 43,170 - +3.8 43,178 + -0.8 43,178 + +3.9 43,178 + +0.1 43,178 + +0.6 43,178 + +1.8 43,178 + +4.5 43,178 + -0.8 43,179 - -1.4 43,179 - -1.0 43,179 - +2.2 43,180 + -0.3 43,180 + +3.0 43,180 + -1.9 43,180 + -1.3 43,181 - -1.4 43,181 - +0.4 43,181 - +0.0 43,230 + -1.1 43,230 + -0.9 43,260 - -0.2 43,374 + Psyr_0041 0.54 +2.5 43,374 + Psyr_0041 0.54 +3.0 43,375 - Psyr_0041 0.54 +1.2 43,394 + Psyr_0041 0.62 -0.4 43,461 + +4.1 43,461 + -1.2 43,461 + +2.5 43,461 + -1.1 43,461 + +1.8 43,461 + +0.1 43,461 + +3.2 43,461 + +0.2 43,461 + -3.3 43,461 + -2.0 43,462 - +0.9 43,462 - -2.0 43,462 - +0.4 43,462 - -0.5 43,462 - -0.9 43,462 - -2.1
Or see this region's nucleotide sequence