Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0031 and Psyr_0032 are separated by 266 nucleotides Psyr_0032 and Psyr_0033 are separated by 125 nucleotides Psyr_0033 and Psyr_0034 overlap by 4 nucleotides
Psyr_0031: Psyr_0031 - Alpha/beta hydrolase fold protein, at 35,445 to 36,269
_0031
Psyr_0032: Psyr_0032 - conserved hypothetical protein, at 36,536 to 37,066
_0032
Psyr_0033: Psyr_0033 - tryptophan synthase, alpha chain, at 37,192 to 38,001
_0033
Psyr_0034: Psyr_0034 - tryptophan synthase, beta chain, at 37,998 to 39,227
_0034
Position (kb) 36
37
38 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 35.564 kb on + strand, within Psyr_0031 at 35.564 kb on + strand, within Psyr_0031 at 35.716 kb on + strand, within Psyr_0031 at 35.716 kb on + strand, within Psyr_0031 at 35.717 kb on - strand, within Psyr_0031 at 35.802 kb on + strand, within Psyr_0031 at 35.802 kb on + strand, within Psyr_0031 at 35.803 kb on - strand, within Psyr_0031 at 35.803 kb on - strand, within Psyr_0031 at 35.803 kb on - strand, within Psyr_0031 at 35.835 kb on + strand, within Psyr_0031 at 35.835 kb on + strand, within Psyr_0031 at 35.838 kb on + strand, within Psyr_0031 at 35.838 kb on + strand, within Psyr_0031 at 35.838 kb on + strand, within Psyr_0031 at 35.838 kb on + strand, within Psyr_0031 at 35.838 kb on + strand, within Psyr_0031 at 35.838 kb on + strand, within Psyr_0031 at 35.838 kb on + strand, within Psyr_0031 at 35.838 kb on + strand, within Psyr_0031 at 35.839 kb on - strand, within Psyr_0031 at 35.839 kb on - strand, within Psyr_0031 at 35.839 kb on - strand, within Psyr_0031 at 35.839 kb on - strand, within Psyr_0031 at 35.839 kb on - strand, within Psyr_0031 at 35.839 kb on - strand, within Psyr_0031 at 35.927 kb on + strand, within Psyr_0031 at 35.949 kb on + strand, within Psyr_0031 at 35.950 kb on - strand, within Psyr_0031 at 35.950 kb on - strand, within Psyr_0031 at 35.950 kb on - strand, within Psyr_0031 at 35.950 kb on - strand, within Psyr_0031 at 35.950 kb on - strand, within Psyr_0031 at 36.162 kb on + strand, within Psyr_0031 at 36.162 kb on + strand, within Psyr_0031 at 36.162 kb on + strand, within Psyr_0031 at 36.163 kb on - strand, within Psyr_0031 at 36.163 kb on - strand, within Psyr_0031 at 36.163 kb on - strand, within Psyr_0031 at 36.327 kb on + strand at 36.327 kb on + strand at 36.328 kb on - strand at 36.328 kb on - strand at 36.328 kb on - strand at 36.340 kb on + strand at 36.340 kb on + strand at 36.341 kb on - strand at 36.341 kb on - strand at 36.342 kb on + strand at 36.454 kb on + strand at 36.454 kb on + strand at 36.454 kb on + strand at 36.454 kb on + strand at 36.454 kb on + strand at 36.454 kb on + strand at 36.454 kb on + strand at 36.454 kb on + strand at 36.454 kb on + strand at 36.454 kb on + strand at 36.454 kb on + strand at 36.455 kb on - strand at 36.455 kb on - strand at 36.455 kb on - strand at 36.455 kb on - strand at 36.456 kb on + strand at 36.564 kb on + strand at 36.564 kb on + strand at 36.633 kb on + strand, within Psyr_0032 at 36.633 kb on + strand, within Psyr_0032 at 36.633 kb on + strand, within Psyr_0032 at 36.633 kb on + strand, within Psyr_0032 at 36.634 kb on - strand, within Psyr_0032 at 36.634 kb on - strand, within Psyr_0032 at 36.634 kb on - strand, within Psyr_0032 at 36.634 kb on - strand, within Psyr_0032 at 36.634 kb on - strand, within Psyr_0032 at 36.645 kb on + strand, within Psyr_0032 at 36.646 kb on - strand, within Psyr_0032 at 36.713 kb on + strand, within Psyr_0032 at 36.713 kb on + strand, within Psyr_0032 at 36.713 kb on + strand, within Psyr_0032 at 36.714 kb on - strand, within Psyr_0032 at 36.714 kb on - strand, within Psyr_0032 at 36.902 kb on - strand, within Psyr_0032 at 37.045 kb on + strand at 37.140 kb on + strand at 37.140 kb on + strand at 37.140 kb on + strand at 37.140 kb on + strand at 37.140 kb on + strand at 37.140 kb on + strand at 37.140 kb on + strand at 37.140 kb on + strand at 37.140 kb on + strand at 37.140 kb on + strand at 37.140 kb on + strand at 37.141 kb on - strand at 37.141 kb on - strand at 37.141 kb on - strand at 37.141 kb on - strand at 37.141 kb on - strand at 37.141 kb on - strand at 37.141 kb on - strand at 37.141 kb on - strand at 37.141 kb on - strand at 37.141 kb on - strand at 37.144 kb on + strand at 37.144 kb on + strand at 37.144 kb on + strand at 37.144 kb on + strand at 37.144 kb on + strand at 37.144 kb on + strand at 37.144 kb on + strand at 37.145 kb on - strand at 37.145 kb on - strand at 37.145 kb on - strand at 37.145 kb on - strand at 37.145 kb on - strand at 37.145 kb on - strand at 37.145 kb on - strand at 37.145 kb on - strand at 37.145 kb on - strand at 37.176 kb on - strand at 37.207 kb on - strand at 37.207 kb on - strand at 37.210 kb on + strand at 37.210 kb on + strand at 37.211 kb on - strand at 37.211 kb on - strand at 37.211 kb on - strand at 37.211 kb on - strand at 37.211 kb on - strand at 37.475 kb on + strand, within Psyr_0033 at 37.475 kb on + strand, within Psyr_0033 at 37.475 kb on + strand, within Psyr_0033 at 37.475 kb on + strand, within Psyr_0033 at 37.476 kb on - strand, within Psyr_0033 at 37.476 kb on - strand, within Psyr_0033 at 37.476 kb on - strand, within Psyr_0033 at 37.478 kb on + strand, within Psyr_0033 at 37.695 kb on - strand, within Psyr_0033 at 37.695 kb on - strand, within Psyr_0033 at 37.695 kb on - strand, within Psyr_0033 at 37.695 kb on - strand, within Psyr_0033 at 37.697 kb on + strand, within Psyr_0033 at 37.991 kb on - strand at 37.991 kb on - strand at 38.002 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 35,564 + Psyr_0031 0.14 -0.2 35,564 + Psyr_0031 0.14 -1.6 35,716 + Psyr_0031 0.33 -1.1 35,716 + Psyr_0031 0.33 -0.4 35,717 - Psyr_0031 0.33 -2.2 35,802 + Psyr_0031 0.43 +4.0 35,802 + Psyr_0031 0.43 -1.0 35,803 - Psyr_0031 0.43 +0.4 35,803 - Psyr_0031 0.43 +2.8 35,803 - Psyr_0031 0.43 -3.6 35,835 + Psyr_0031 0.47 +2.5 35,835 + Psyr_0031 0.47 +1.0 35,838 + Psyr_0031 0.48 +0.9 35,838 + Psyr_0031 0.48 -0.3 35,838 + Psyr_0031 0.48 +0.3 35,838 + Psyr_0031 0.48 +0.7 35,838 + Psyr_0031 0.48 -1.7 35,838 + Psyr_0031 0.48 -0.1 35,838 + Psyr_0031 0.48 +0.1 35,838 + Psyr_0031 0.48 -1.4 35,839 - Psyr_0031 0.48 -1.9 35,839 - Psyr_0031 0.48 +0.2 35,839 - Psyr_0031 0.48 -0.3 35,839 - Psyr_0031 0.48 +3.7 35,839 - Psyr_0031 0.48 -2.7 35,839 - Psyr_0031 0.48 +0.3 35,927 + Psyr_0031 0.58 -0.2 35,949 + Psyr_0031 0.61 +0.5 35,950 - Psyr_0031 0.61 -1.2 35,950 - Psyr_0031 0.61 -1.2 35,950 - Psyr_0031 0.61 +2.2 35,950 - Psyr_0031 0.61 +0.6 35,950 - Psyr_0031 0.61 +4.0 36,162 + Psyr_0031 0.87 +0.5 36,162 + Psyr_0031 0.87 -1.6 36,162 + Psyr_0031 0.87 +0.1 36,163 - Psyr_0031 0.87 +2.2 36,163 - Psyr_0031 0.87 -0.5 36,163 - Psyr_0031 0.87 +0.6 36,327 + +2.2 36,327 + -0.6 36,328 - -2.3 36,328 - +2.3 36,328 - +3.8 36,340 + -0.7 36,340 + +0.2 36,341 - -0.6 36,341 - -2.6 36,342 + +4.0 36,454 + +1.0 36,454 + +2.5 36,454 + -3.5 36,454 + +1.8 36,454 + -1.2 36,454 + -1.9 36,454 + -1.7 36,454 + -1.1 36,454 + +0.4 36,454 + +1.0 36,454 + -2.1 36,455 - +4.3 36,455 - -3.0 36,455 - +0.0 36,455 - -0.4 36,456 + -0.9 36,564 + +1.5 36,564 + -0.7 36,633 + Psyr_0032 0.18 +2.2 36,633 + Psyr_0032 0.18 +2.8 36,633 + Psyr_0032 0.18 +1.3 36,633 + Psyr_0032 0.18 +1.8 36,634 - Psyr_0032 0.18 +1.7 36,634 - Psyr_0032 0.18 +3.8 36,634 - Psyr_0032 0.18 +0.8 36,634 - Psyr_0032 0.18 +1.4 36,634 - Psyr_0032 0.18 -1.7 36,645 + Psyr_0032 0.21 -0.1 36,646 - Psyr_0032 0.21 +1.8 36,713 + Psyr_0032 0.33 +2.2 36,713 + Psyr_0032 0.33 -1.7 36,713 + Psyr_0032 0.33 +0.8 36,714 - Psyr_0032 0.34 -1.8 36,714 - Psyr_0032 0.34 +3.5 36,902 - Psyr_0032 0.69 -3.4 37,045 + -1.4 37,140 + -2.3 37,140 + -3.1 37,140 + +2.5 37,140 + -1.3 37,140 + +0.7 37,140 + -1.0 37,140 + -0.1 37,140 + +3.4 37,140 + +0.7 37,140 + -2.0 37,140 + +0.3 37,141 - -4.0 37,141 - +1.1 37,141 - +3.2 37,141 - -0.1 37,141 - +4.5 37,141 - -0.4 37,141 - -2.1 37,141 - -0.5 37,141 - -0.3 37,141 - +1.7 37,144 + +0.6 37,144 + +0.3 37,144 + +0.2 37,144 + -1.4 37,144 + +2.8 37,144 + -1.1 37,144 + -0.1 37,145 - -1.2 37,145 - -0.6 37,145 - +0.2 37,145 - -1.2 37,145 - -0.1 37,145 - -0.5 37,145 - +1.2 37,145 - -1.1 37,145 - -1.2 37,176 - +3.8 37,207 - -1.5 37,207 - -1.9 37,210 + +0.5 37,210 + -2.0 37,211 - +3.8 37,211 - +4.0 37,211 - +0.1 37,211 - +4.3 37,211 - +0.2 37,475 + Psyr_0033 0.35 -3.5 37,475 + Psyr_0033 0.35 -4.0 37,475 + Psyr_0033 0.35 -3.7 37,475 + Psyr_0033 0.35 +0.2 37,476 - Psyr_0033 0.35 +2.8 37,476 - Psyr_0033 0.35 +3.9 37,476 - Psyr_0033 0.35 +4.5 37,478 + Psyr_0033 0.35 +0.2 37,695 - Psyr_0033 0.62 -1.8 37,695 - Psyr_0033 0.62 -1.8 37,695 - Psyr_0033 0.62 -2.1 37,695 - Psyr_0033 0.62 +4.3 37,697 + Psyr_0033 0.62 -1.7 37,991 - +3.2 37,991 - +0.8 38,002 - -1.6
Or see this region's nucleotide sequence