Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0015 and Psyr_0016 are separated by 153 nucleotides Psyr_0016 and Psyr_0017 overlap by 4 nucleotides
Psyr_0015: Psyr_0015 - Oxidoreductase alpha (molybdopterin) subunit, at 16,812 to 19,175
_0015
Psyr_0016: Psyr_0016 - TrkA-N:TrkA-C, at 19,329 to 20,702
_0016
Psyr_0017: Psyr_0017 - 16S rRNA m(5)C-967 methyltransferase, at 20,699 to 22,045
_0017
Position (kb) 19
20
21 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 18.640 kb on + strand, within Psyr_0015 at 18.640 kb on + strand, within Psyr_0015 at 18.641 kb on - strand, within Psyr_0015 at 18.641 kb on - strand, within Psyr_0015 at 18.641 kb on - strand, within Psyr_0015 at 18.641 kb on - strand, within Psyr_0015 at 18.661 kb on + strand, within Psyr_0015 at 18.661 kb on + strand, within Psyr_0015 at 18.662 kb on - strand, within Psyr_0015 at 18.700 kb on + strand, within Psyr_0015 at 18.700 kb on + strand, within Psyr_0015 at 18.700 kb on + strand, within Psyr_0015 at 18.700 kb on + strand, within Psyr_0015 at 18.700 kb on + strand, within Psyr_0015 at 18.700 kb on + strand, within Psyr_0015 at 18.700 kb on + strand, within Psyr_0015 at 18.700 kb on + strand, within Psyr_0015 at 18.701 kb on - strand, within Psyr_0015 at 18.701 kb on - strand, within Psyr_0015 at 18.701 kb on - strand, within Psyr_0015 at 18.701 kb on - strand, within Psyr_0015 at 18.701 kb on - strand, within Psyr_0015 at 18.701 kb on - strand, within Psyr_0015 at 18.701 kb on - strand, within Psyr_0015 at 18.784 kb on + strand, within Psyr_0015 at 18.784 kb on + strand, within Psyr_0015 at 18.784 kb on + strand, within Psyr_0015 at 18.784 kb on + strand, within Psyr_0015 at 18.784 kb on + strand, within Psyr_0015 at 18.784 kb on + strand, within Psyr_0015 at 18.784 kb on + strand, within Psyr_0015 at 18.784 kb on + strand, within Psyr_0015 at 18.785 kb on - strand, within Psyr_0015 at 18.785 kb on - strand, within Psyr_0015 at 18.785 kb on - strand, within Psyr_0015 at 18.785 kb on - strand, within Psyr_0015 at 18.824 kb on + strand, within Psyr_0015 at 18.825 kb on - strand, within Psyr_0015 at 18.878 kb on + strand, within Psyr_0015 at 18.878 kb on + strand, within Psyr_0015 at 18.879 kb on - strand, within Psyr_0015 at 18.879 kb on - strand, within Psyr_0015 at 18.956 kb on - strand at 18.956 kb on - strand at 18.956 kb on - strand at 19.194 kb on + strand at 19.231 kb on + strand at 19.231 kb on + strand at 19.232 kb on - strand at 19.302 kb on + strand at 19.302 kb on + strand at 19.389 kb on + strand at 19.389 kb on + strand at 19.389 kb on + strand at 19.389 kb on + strand at 19.390 kb on - strand at 19.390 kb on - strand at 19.703 kb on - strand, within Psyr_0016 at 19.703 kb on - strand, within Psyr_0016 at 19.703 kb on - strand, within Psyr_0016 at 19.900 kb on + strand, within Psyr_0016 at 19.901 kb on - strand, within Psyr_0016 at 20.003 kb on + strand, within Psyr_0016 at 20.003 kb on + strand, within Psyr_0016 at 20.003 kb on + strand, within Psyr_0016 at 20.011 kb on + strand, within Psyr_0016 at 20.011 kb on + strand, within Psyr_0016 at 20.011 kb on + strand, within Psyr_0016 at 20.012 kb on - strand, within Psyr_0016 at 20.012 kb on - strand, within Psyr_0016 at 20.012 kb on - strand, within Psyr_0016 at 20.012 kb on - strand, within Psyr_0016 at 20.012 kb on - strand, within Psyr_0016 at 20.012 kb on - strand, within Psyr_0016 at 20.012 kb on - strand, within Psyr_0016 at 20.012 kb on - strand, within Psyr_0016 at 20.140 kb on + strand, within Psyr_0016 at 20.140 kb on + strand, within Psyr_0016 at 20.140 kb on + strand, within Psyr_0016 at 20.141 kb on - strand, within Psyr_0016 at 20.141 kb on - strand, within Psyr_0016 at 20.185 kb on - strand, within Psyr_0016 at 20.185 kb on - strand, within Psyr_0016 at 20.185 kb on - strand, within Psyr_0016 at 20.185 kb on - strand, within Psyr_0016 at 20.185 kb on - strand, within Psyr_0016 at 20.221 kb on + strand, within Psyr_0016 at 20.221 kb on + strand, within Psyr_0016 at 20.221 kb on + strand, within Psyr_0016 at 20.225 kb on - strand, within Psyr_0016 at 20.311 kb on + strand, within Psyr_0016 at 20.311 kb on + strand, within Psyr_0016 at 20.311 kb on + strand, within Psyr_0016 at 20.311 kb on + strand, within Psyr_0016 at 20.311 kb on + strand, within Psyr_0016 at 20.311 kb on + strand, within Psyr_0016 at 20.311 kb on + strand, within Psyr_0016 at 20.311 kb on + strand, within Psyr_0016 at 20.311 kb on + strand, within Psyr_0016 at 20.311 kb on + strand, within Psyr_0016 at 20.311 kb on + strand, within Psyr_0016 at 20.311 kb on + strand, within Psyr_0016 at 20.312 kb on - strand, within Psyr_0016 at 20.312 kb on - strand, within Psyr_0016 at 20.312 kb on - strand, within Psyr_0016 at 20.312 kb on - strand, within Psyr_0016 at 20.312 kb on - strand, within Psyr_0016 at 20.312 kb on - strand, within Psyr_0016 at 20.312 kb on - strand, within Psyr_0016 at 20.312 kb on - strand, within Psyr_0016 at 20.312 kb on - strand, within Psyr_0016 at 20.312 kb on - strand, within Psyr_0016 at 20.312 kb on - strand, within Psyr_0016 at 20.343 kb on + strand, within Psyr_0016 at 20.343 kb on + strand, within Psyr_0016 at 20.343 kb on + strand, within Psyr_0016 at 20.343 kb on + strand, within Psyr_0016 at 20.343 kb on + strand, within Psyr_0016 at 20.344 kb on - strand, within Psyr_0016 at 20.367 kb on - strand, within Psyr_0016 at 20.392 kb on + strand, within Psyr_0016 at 20.392 kb on + strand, within Psyr_0016 at 20.441 kb on + strand, within Psyr_0016 at 20.441 kb on + strand, within Psyr_0016 at 20.708 kb on + strand at 20.757 kb on + strand at 20.760 kb on + strand at 20.761 kb on - strand at 20.761 kb on - strand at 20.875 kb on + strand, within Psyr_0017 at 20.938 kb on - strand, within Psyr_0017 at 20.938 kb on - strand, within Psyr_0017 at 20.965 kb on + strand, within Psyr_0017 at 20.966 kb on - strand, within Psyr_0017 at 20.966 kb on - strand, within Psyr_0017 at 20.966 kb on - strand, within Psyr_0017 at 21.254 kb on + strand, within Psyr_0017 at 21.255 kb on - strand, within Psyr_0017 at 21.492 kb on + strand, within Psyr_0017 at 21.493 kb on - strand, within Psyr_0017 at 21.493 kb on - strand, within Psyr_0017
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 18,640 + Psyr_0015 0.77 +1.9 18,640 + Psyr_0015 0.77 -2.4 18,641 - Psyr_0015 0.77 +3.2 18,641 - Psyr_0015 0.77 +0.3 18,641 - Psyr_0015 0.77 +3.1 18,641 - Psyr_0015 0.77 -0.8 18,661 + Psyr_0015 0.78 +0.2 18,661 + Psyr_0015 0.78 -0.0 18,662 - Psyr_0015 0.78 -1.2 18,700 + Psyr_0015 0.80 -1.7 18,700 + Psyr_0015 0.80 -1.9 18,700 + Psyr_0015 0.80 +0.2 18,700 + Psyr_0015 0.80 +4.3 18,700 + Psyr_0015 0.80 +1.2 18,700 + Psyr_0015 0.80 -1.8 18,700 + Psyr_0015 0.80 -0.3 18,700 + Psyr_0015 0.80 -1.3 18,701 - Psyr_0015 0.80 +0.1 18,701 - Psyr_0015 0.80 -1.7 18,701 - Psyr_0015 0.80 +4.9 18,701 - Psyr_0015 0.80 -1.8 18,701 - Psyr_0015 0.80 -0.6 18,701 - Psyr_0015 0.80 +2.5 18,701 - Psyr_0015 0.80 +0.9 18,784 + Psyr_0015 0.83 -1.1 18,784 + Psyr_0015 0.83 -0.5 18,784 + Psyr_0015 0.83 +1.1 18,784 + Psyr_0015 0.83 +1.8 18,784 + Psyr_0015 0.83 +0.7 18,784 + Psyr_0015 0.83 +0.9 18,784 + Psyr_0015 0.83 -0.8 18,784 + Psyr_0015 0.83 +0.2 18,785 - Psyr_0015 0.83 +0.2 18,785 - Psyr_0015 0.83 +1.0 18,785 - Psyr_0015 0.83 -2.2 18,785 - Psyr_0015 0.83 -0.4 18,824 + Psyr_0015 0.85 +3.9 18,825 - Psyr_0015 0.85 -0.4 18,878 + Psyr_0015 0.87 +0.0 18,878 + Psyr_0015 0.87 -0.4 18,879 - Psyr_0015 0.87 +1.2 18,879 - Psyr_0015 0.87 -0.2 18,956 - -0.4 18,956 - -2.3 18,956 - +1.3 19,194 + +5.2 19,231 + -4.7 19,231 + -0.1 19,232 - +4.7 19,302 + +1.1 19,302 + -0.1 19,389 + -1.0 19,389 + -0.8 19,389 + +3.0 19,389 + +2.2 19,390 - -1.9 19,390 - +0.2 19,703 - Psyr_0016 0.27 -1.2 19,703 - Psyr_0016 0.27 +1.2 19,703 - Psyr_0016 0.27 +0.6 19,900 + Psyr_0016 0.42 +2.2 19,901 - Psyr_0016 0.42 -1.9 20,003 + Psyr_0016 0.49 +5.3 20,003 + Psyr_0016 0.49 -0.2 20,003 + Psyr_0016 0.49 -0.6 20,011 + Psyr_0016 0.50 -1.0 20,011 + Psyr_0016 0.50 +1.2 20,011 + Psyr_0016 0.50 +1.8 20,012 - Psyr_0016 0.50 -2.3 20,012 - Psyr_0016 0.50 +0.2 20,012 - Psyr_0016 0.50 +1.0 20,012 - Psyr_0016 0.50 +2.8 20,012 - Psyr_0016 0.50 +3.9 20,012 - Psyr_0016 0.50 +0.2 20,012 - Psyr_0016 0.50 -3.4 20,012 - Psyr_0016 0.50 +1.2 20,140 + Psyr_0016 0.59 -0.1 20,140 + Psyr_0016 0.59 +0.4 20,140 + Psyr_0016 0.59 +0.4 20,141 - Psyr_0016 0.59 +2.5 20,141 - Psyr_0016 0.59 -1.0 20,185 - Psyr_0016 0.62 +0.2 20,185 - Psyr_0016 0.62 -1.3 20,185 - Psyr_0016 0.62 +0.0 20,185 - Psyr_0016 0.62 -0.1 20,185 - Psyr_0016 0.62 -1.9 20,221 + Psyr_0016 0.65 +0.3 20,221 + Psyr_0016 0.65 -2.2 20,221 + Psyr_0016 0.65 -1.8 20,225 - Psyr_0016 0.65 -2.5 20,311 + Psyr_0016 0.71 -1.3 20,311 + Psyr_0016 0.71 +1.7 20,311 + Psyr_0016 0.71 -2.0 20,311 + Psyr_0016 0.71 -3.4 20,311 + Psyr_0016 0.71 -1.7 20,311 + Psyr_0016 0.71 -1.2 20,311 + Psyr_0016 0.71 -0.8 20,311 + Psyr_0016 0.71 +0.7 20,311 + Psyr_0016 0.71 +0.8 20,311 + Psyr_0016 0.71 +2.2 20,311 + Psyr_0016 0.71 +1.2 20,311 + Psyr_0016 0.71 +0.8 20,312 - Psyr_0016 0.72 +1.2 20,312 - Psyr_0016 0.72 -1.9 20,312 - Psyr_0016 0.72 +0.2 20,312 - Psyr_0016 0.72 -0.8 20,312 - Psyr_0016 0.72 +2.5 20,312 - Psyr_0016 0.72 +2.5 20,312 - Psyr_0016 0.72 -3.3 20,312 - Psyr_0016 0.72 -1.7 20,312 - Psyr_0016 0.72 +4.1 20,312 - Psyr_0016 0.72 +1.2 20,312 - Psyr_0016 0.72 -1.5 20,343 + Psyr_0016 0.74 -1.8 20,343 + Psyr_0016 0.74 -1.9 20,343 + Psyr_0016 0.74 +0.1 20,343 + Psyr_0016 0.74 -1.8 20,343 + Psyr_0016 0.74 +3.0 20,344 - Psyr_0016 0.74 +2.5 20,367 - Psyr_0016 0.76 +1.9 20,392 + Psyr_0016 0.77 -3.4 20,392 + Psyr_0016 0.77 +0.9 20,441 + Psyr_0016 0.81 -0.3 20,441 + Psyr_0016 0.81 -1.7 20,708 + -1.5 20,757 + -4.6 20,760 + +3.8 20,761 - +1.5 20,761 - -0.6 20,875 + Psyr_0017 0.13 +0.1 20,938 - Psyr_0017 0.18 +2.8 20,938 - Psyr_0017 0.18 -1.7 20,965 + Psyr_0017 0.20 -1.4 20,966 - Psyr_0017 0.20 +3.2 20,966 - Psyr_0017 0.20 +3.2 20,966 - Psyr_0017 0.20 -1.5 21,254 + Psyr_0017 0.41 -0.2 21,255 - Psyr_0017 0.41 +0.2 21,492 + Psyr_0017 0.59 -2.0 21,493 - Psyr_0017 0.59 -1.6 21,493 - Psyr_0017 0.59 -1.3
Or see this region's nucleotide sequence