Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0007 and Psyr_0008 are separated by 166 nucleotides Psyr_0008 and Psyr_0009 are separated by 140 nucleotides Psyr_0009 and Psyr_0010 are separated by 65 nucleotides
Psyr_0007: Psyr_0007 - Aliphatic nitrilase, at 8,233 to 9,243
_0007
Psyr_0008: Psyr_0008 - transcriptional regulator, AraC family, at 9,410 to 10,384
_0008
Psyr_0009: Psyr_0009 - Phospholipid/glycerol acyltransferase, at 10,525 to 11,295
_0009
Psyr_0010: Psyr_0010 - D-alpha,beta-D-heptose 1,7-bisphosphate phosphatase, at 11,361 to 11,903
_0010
Position (kb)
9
10
11 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 8.427 kb on - strand, within Psyr_0007 at 8.616 kb on - strand, within Psyr_0007 at 8.616 kb on - strand, within Psyr_0007 at 8.723 kb on + strand, within Psyr_0007 at 8.723 kb on + strand, within Psyr_0007 at 8.723 kb on + strand, within Psyr_0007 at 8.723 kb on + strand, within Psyr_0007 at 8.723 kb on + strand, within Psyr_0007 at 8.723 kb on + strand, within Psyr_0007 at 8.723 kb on + strand, within Psyr_0007 at 8.723 kb on + strand, within Psyr_0007 at 8.723 kb on + strand, within Psyr_0007 at 8.723 kb on + strand, within Psyr_0007 at 8.723 kb on + strand, within Psyr_0007 at 8.723 kb on + strand, within Psyr_0007 at 8.723 kb on + strand, within Psyr_0007 at 8.723 kb on + strand, within Psyr_0007 at 8.724 kb on - strand, within Psyr_0007 at 8.724 kb on - strand, within Psyr_0007 at 8.724 kb on - strand, within Psyr_0007 at 8.724 kb on - strand, within Psyr_0007 at 8.724 kb on - strand, within Psyr_0007 at 8.724 kb on - strand, within Psyr_0007 at 8.724 kb on - strand, within Psyr_0007 at 8.732 kb on + strand, within Psyr_0007 at 8.732 kb on + strand, within Psyr_0007 at 8.732 kb on + strand, within Psyr_0007 at 8.803 kb on - strand, within Psyr_0007 at 8.803 kb on - strand, within Psyr_0007 at 8.838 kb on + strand, within Psyr_0007 at 8.838 kb on + strand, within Psyr_0007 at 8.838 kb on + strand, within Psyr_0007 at 8.838 kb on + strand, within Psyr_0007 at 8.854 kb on - strand, within Psyr_0007 at 8.917 kb on - strand, within Psyr_0007 at 8.918 kb on + strand, within Psyr_0007 at 8.918 kb on + strand, within Psyr_0007 at 8.918 kb on + strand, within Psyr_0007 at 8.918 kb on + strand, within Psyr_0007 at 8.918 kb on + strand, within Psyr_0007 at 8.918 kb on + strand, within Psyr_0007 at 8.918 kb on + strand, within Psyr_0007 at 8.918 kb on + strand, within Psyr_0007 at 8.918 kb on + strand, within Psyr_0007 at 8.918 kb on + strand, within Psyr_0007 at 8.919 kb on - strand, within Psyr_0007 at 8.919 kb on - strand, within Psyr_0007 at 8.919 kb on - strand, within Psyr_0007 at 8.919 kb on - strand, within Psyr_0007 at 8.919 kb on - strand, within Psyr_0007 at 8.919 kb on - strand, within Psyr_0007 at 8.919 kb on - strand, within Psyr_0007 at 8.919 kb on - strand, within Psyr_0007 at 8.919 kb on - strand, within Psyr_0007 at 8.919 kb on - strand, within Psyr_0007 at 8.946 kb on - strand, within Psyr_0007 at 8.960 kb on + strand, within Psyr_0007 at 8.960 kb on + strand, within Psyr_0007 at 8.960 kb on + strand, within Psyr_0007 at 8.960 kb on + strand, within Psyr_0007 at 8.960 kb on + strand, within Psyr_0007 at 8.960 kb on + strand, within Psyr_0007 at 8.960 kb on + strand, within Psyr_0007 at 8.960 kb on + strand, within Psyr_0007 at 8.961 kb on - strand, within Psyr_0007 at 8.961 kb on - strand, within Psyr_0007 at 8.961 kb on - strand, within Psyr_0007 at 8.961 kb on - strand, within Psyr_0007 at 8.961 kb on - strand, within Psyr_0007 at 8.991 kb on + strand, within Psyr_0007 at 8.991 kb on + strand, within Psyr_0007 at 8.991 kb on + strand at 8.991 kb on + strand, within Psyr_0007 at 8.992 kb on - strand, within Psyr_0007 at 8.992 kb on - strand, within Psyr_0007 at 9.036 kb on - strand, within Psyr_0007 at 9.123 kb on + strand, within Psyr_0007 at 9.123 kb on + strand, within Psyr_0007 at 9.123 kb on + strand, within Psyr_0007 at 9.124 kb on - strand, within Psyr_0007 at 9.124 kb on - strand, within Psyr_0007 at 9.175 kb on + strand at 9.176 kb on - strand at 9.280 kb on + strand at 9.280 kb on + strand at 9.280 kb on + strand at 9.301 kb on + strand at 9.301 kb on + strand at 9.302 kb on - strand at 9.302 kb on - strand at 9.305 kb on - strand at 9.305 kb on - strand at 9.305 kb on - strand at 9.341 kb on - strand at 9.419 kb on + strand at 9.419 kb on + strand at 9.419 kb on + strand at 9.419 kb on + strand at 9.419 kb on + strand at 9.420 kb on - strand at 9.420 kb on - strand at 9.420 kb on - strand at 9.420 kb on - strand at 9.484 kb on + strand at 9.485 kb on - strand at 9.485 kb on - strand at 9.551 kb on - strand, within Psyr_0008 at 9.579 kb on + strand, within Psyr_0008 at 9.579 kb on + strand, within Psyr_0008 at 9.579 kb on + strand, within Psyr_0008 at 9.579 kb on + strand, within Psyr_0008 at 9.580 kb on - strand, within Psyr_0008 at 9.580 kb on - strand, within Psyr_0008 at 9.580 kb on - strand, within Psyr_0008 at 9.580 kb on - strand, within Psyr_0008 at 9.580 kb on - strand, within Psyr_0008 at 9.610 kb on + strand, within Psyr_0008 at 9.610 kb on + strand, within Psyr_0008 at 9.610 kb on + strand, within Psyr_0008 at 9.610 kb on + strand, within Psyr_0008 at 9.610 kb on + strand, within Psyr_0008 at 9.611 kb on - strand, within Psyr_0008 at 9.619 kb on + strand, within Psyr_0008 at 9.645 kb on + strand, within Psyr_0008 at 9.727 kb on + strand, within Psyr_0008 at 9.727 kb on + strand, within Psyr_0008 at 9.728 kb on - strand, within Psyr_0008 at 9.731 kb on + strand, within Psyr_0008 at 9.731 kb on + strand, within Psyr_0008 at 9.731 kb on + strand, within Psyr_0008 at 9.731 kb on + strand, within Psyr_0008 at 9.731 kb on + strand, within Psyr_0008 at 9.731 kb on + strand, within Psyr_0008 at 9.836 kb on + strand, within Psyr_0008 at 9.837 kb on - strand, within Psyr_0008 at 9.837 kb on - strand, within Psyr_0008 at 10.035 kb on + strand, within Psyr_0008 at 10.036 kb on - strand, within Psyr_0008 at 10.048 kb on + strand, within Psyr_0008 at 10.049 kb on - strand, within Psyr_0008 at 10.057 kb on + strand, within Psyr_0008 at 10.058 kb on - strand, within Psyr_0008 at 10.078 kb on + strand, within Psyr_0008 at 10.079 kb on - strand, within Psyr_0008 at 10.079 kb on - strand, within Psyr_0008 at 10.079 kb on - strand, within Psyr_0008 at 10.079 kb on - strand, within Psyr_0008 at 10.079 kb on - strand, within Psyr_0008 at 10.130 kb on + strand, within Psyr_0008 at 10.130 kb on + strand, within Psyr_0008 at 10.130 kb on + strand, within Psyr_0008 at 10.131 kb on - strand, within Psyr_0008 at 10.131 kb on - strand, within Psyr_0008 at 10.131 kb on - strand, within Psyr_0008 at 10.175 kb on + strand, within Psyr_0008 at 10.194 kb on + strand, within Psyr_0008 at 10.195 kb on - strand, within Psyr_0008 at 10.286 kb on + strand, within Psyr_0008 at 10.287 kb on - strand at 10.287 kb on - strand at 10.287 kb on - strand at 10.287 kb on - strand at 10.287 kb on - strand at 10.287 kb on - strand at 10.366 kb on + strand at 10.382 kb on + strand at 10.399 kb on + strand at 10.399 kb on + strand at 10.400 kb on - strand at 10.414 kb on + strand at 10.455 kb on - strand at 10.456 kb on + strand at 10.506 kb on - strand at 10.506 kb on - strand at 10.656 kb on - strand, within Psyr_0009 at 11.296 kb on - strand at 11.296 kb on - strand at 11.296 kb on - strand at 11.296 kb on - strand at 11.296 kb on - strand at 11.296 kb on - strand at 11.296 kb on - strand at 11.300 kb on - strand at 11.300 kb on - strand at 11.300 kb on - strand at 11.300 kb on - strand at 11.300 kb on - strand at 11.300 kb on - strand at 11.300 kb on - strand at 11.300 kb on - strand at 11.300 kb on - strand at 11.300 kb on - strand at 11.300 kb on - strand at 11.300 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 8,427 - Psyr_0007 0.19 -1.5 8,616 - Psyr_0007 0.38 -0.0 8,616 - Psyr_0007 0.38 -1.7 8,723 + Psyr_0007 0.48 +1.2 8,723 + Psyr_0007 0.48 -0.5 8,723 + Psyr_0007 0.48 -1.5 8,723 + Psyr_0007 0.48 -0.1 8,723 + Psyr_0007 0.48 -1.5 8,723 + Psyr_0007 0.48 +0.7 8,723 + Psyr_0007 0.48 +2.2 8,723 + Psyr_0007 0.48 -4.1 8,723 + Psyr_0007 0.48 +0.1 8,723 + Psyr_0007 0.48 -1.0 8,723 + Psyr_0007 0.48 -2.5 8,723 + Psyr_0007 0.48 -0.7 8,723 + Psyr_0007 0.48 -1.5 8,723 + Psyr_0007 0.48 -0.5 8,724 - Psyr_0007 0.49 -0.5 8,724 - Psyr_0007 0.49 +0.3 8,724 - Psyr_0007 0.49 +0.1 8,724 - Psyr_0007 0.49 -1.6 8,724 - Psyr_0007 0.49 -0.9 8,724 - Psyr_0007 0.49 -1.4 8,724 - Psyr_0007 0.49 -1.1 8,732 + Psyr_0007 0.49 -1.8 8,732 + Psyr_0007 0.49 +1.0 8,732 + Psyr_0007 0.49 -0.4 8,803 - Psyr_0007 0.56 +0.2 8,803 - Psyr_0007 0.56 -0.6 8,838 + Psyr_0007 0.60 -1.0 8,838 + Psyr_0007 0.60 +4.0 8,838 + Psyr_0007 0.60 -2.1 8,838 + Psyr_0007 0.60 -1.2 8,854 - Psyr_0007 0.61 -1.9 8,917 - Psyr_0007 0.68 -1.0 8,918 + Psyr_0007 0.68 -0.9 8,918 + Psyr_0007 0.68 +1.3 8,918 + Psyr_0007 0.68 +0.2 8,918 + Psyr_0007 0.68 -1.0 8,918 + Psyr_0007 0.68 +0.2 8,918 + Psyr_0007 0.68 -1.0 8,918 + Psyr_0007 0.68 +3.5 8,918 + Psyr_0007 0.68 -3.1 8,918 + Psyr_0007 0.68 -2.0 8,918 + Psyr_0007 0.68 -0.9 8,919 - Psyr_0007 0.68 -1.3 8,919 - Psyr_0007 0.68 -0.1 8,919 - Psyr_0007 0.68 +1.0 8,919 - Psyr_0007 0.68 -0.8 8,919 - Psyr_0007 0.68 -1.9 8,919 - Psyr_0007 0.68 -0.5 8,919 - Psyr_0007 0.68 +0.4 8,919 - Psyr_0007 0.68 -0.4 8,919 - Psyr_0007 0.68 +2.2 8,919 - Psyr_0007 0.68 -0.2 8,946 - Psyr_0007 0.71 +3.4 8,960 + Psyr_0007 0.72 -1.1 8,960 + Psyr_0007 0.72 +3.4 8,960 + Psyr_0007 0.72 -0.5 8,960 + Psyr_0007 0.72 -3.0 8,960 + Psyr_0007 0.72 +0.2 8,960 + Psyr_0007 0.72 +0.6 8,960 + Psyr_0007 0.72 -1.3 8,960 + Psyr_0007 0.72 +0.9 8,961 - Psyr_0007 0.72 -1.5 8,961 - Psyr_0007 0.72 -1.7 8,961 - Psyr_0007 0.72 +3.2 8,961 - Psyr_0007 0.72 -2.8 8,961 - Psyr_0007 0.72 +3.4 8,991 + Psyr_0007 0.75 -0.2 8,991 + Psyr_0007 0.75 +0.2 8,991 + -3.0 8,991 + Psyr_0007 0.75 +2.8 8,992 - Psyr_0007 0.75 -2.4 8,992 - Psyr_0007 0.75 -0.4 9,036 - Psyr_0007 0.79 -1.3 9,123 + Psyr_0007 0.88 -1.1 9,123 + Psyr_0007 0.88 +3.7 9,123 + Psyr_0007 0.88 -0.2 9,124 - Psyr_0007 0.88 -0.3 9,124 - Psyr_0007 0.88 -1.4 9,175 + +2.1 9,176 - +2.5 9,280 + -0.8 9,280 + -0.0 9,280 + +1.2 9,301 + +0.1 9,301 + +0.1 9,302 - -0.7 9,302 - +0.4 9,305 - +4.3 9,305 - -0.4 9,305 - -0.1 9,341 - -1.8 9,419 + -0.9 9,419 + +0.4 9,419 + -2.3 9,419 + -0.3 9,419 + -0.8 9,420 - -2.2 9,420 - -0.5 9,420 - +4.2 9,420 - -1.3 9,484 + -3.8 9,485 - +2.5 9,485 - +2.5 9,551 - Psyr_0008 0.14 -1.0 9,579 + Psyr_0008 0.17 -1.7 9,579 + Psyr_0008 0.17 +2.2 9,579 + Psyr_0008 0.17 +0.2 9,579 + Psyr_0008 0.17 +5.1 9,580 - Psyr_0008 0.17 +2.8 9,580 - Psyr_0008 0.17 +2.2 9,580 - Psyr_0008 0.17 -2.6 9,580 - Psyr_0008 0.17 -1.0 9,580 - Psyr_0008 0.17 +0.2 9,610 + Psyr_0008 0.21 +0.8 9,610 + Psyr_0008 0.21 -0.2 9,610 + Psyr_0008 0.21 +0.9 9,610 + Psyr_0008 0.21 -0.7 9,610 + Psyr_0008 0.21 -0.3 9,611 - Psyr_0008 0.21 +0.2 9,619 + Psyr_0008 0.21 -1.3 9,645 + Psyr_0008 0.24 +1.6 9,727 + Psyr_0008 0.33 +1.8 9,727 + Psyr_0008 0.33 -2.3 9,728 - Psyr_0008 0.33 -0.8 9,731 + Psyr_0008 0.33 +3.8 9,731 + Psyr_0008 0.33 +1.8 9,731 + Psyr_0008 0.33 +2.8 9,731 + Psyr_0008 0.33 +1.0 9,731 + Psyr_0008 0.33 +3.0 9,731 + Psyr_0008 0.33 +3.8 9,836 + Psyr_0008 0.44 -1.3 9,837 - Psyr_0008 0.44 -0.9 9,837 - Psyr_0008 0.44 +0.2 10,035 + Psyr_0008 0.64 +1.2 10,036 - Psyr_0008 0.64 -1.4 10,048 + Psyr_0008 0.65 +0.3 10,049 - Psyr_0008 0.66 +1.2 10,057 + Psyr_0008 0.66 -1.9 10,058 - Psyr_0008 0.66 -1.3 10,078 + Psyr_0008 0.69 +3.0 10,079 - Psyr_0008 0.69 -0.5 10,079 - Psyr_0008 0.69 -0.8 10,079 - Psyr_0008 0.69 +0.4 10,079 - Psyr_0008 0.69 -0.4 10,079 - Psyr_0008 0.69 -0.7 10,130 + Psyr_0008 0.74 -1.2 10,130 + Psyr_0008 0.74 +5.6 10,130 + Psyr_0008 0.74 -0.3 10,131 - Psyr_0008 0.74 +3.8 10,131 - Psyr_0008 0.74 +1.3 10,131 - Psyr_0008 0.74 +3.0 10,175 + Psyr_0008 0.78 +1.2 10,194 + Psyr_0008 0.80 +0.6 10,195 - Psyr_0008 0.81 -1.5 10,286 + Psyr_0008 0.90 +2.1 10,287 - -4.1 10,287 - +3.2 10,287 - +1.2 10,287 - +3.2 10,287 - -1.3 10,287 - -1.0 10,366 + +0.8 10,382 + -2.5 10,399 + +0.2 10,399 + -0.1 10,400 - +0.2 10,414 + -1.8 10,455 - -0.7 10,456 + -0.9 10,506 - -0.1 10,506 - -0.7 10,656 - Psyr_0009 0.17 -3.0 11,296 - +0.2 11,296 - -3.0 11,296 - -3.1 11,296 - +3.7 11,296 - +0.2 11,296 - +2.2 11,296 - +0.2 11,300 - -0.7 11,300 - +0.2 11,300 - -0.5 11,300 - +1.5 11,300 - +2.5 11,300 - -0.3 11,300 - -3.6 11,300 - -1.0 11,300 - -1.3 11,300 - -0.5 11,300 - -1.1 11,300 - +2.5
Or see this region's nucleotide sequence