Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5976 and Pf1N1B4_5977 are separated by 71 nucleotides Pf1N1B4_5977 and Pf1N1B4_5978 are separated by 212 nucleotides
Pf1N1B4_5976 - 1-pyrroline-4-hydroxy-2-carboxylate deaminase (EC 3.5.4.22), at 423,817 to 424,734
_5976
Pf1N1B4_5977 - Transporter, MFS superfamily, at 424,806 to 426,203
_5977
Pf1N1B4_5978 - Ketoglutarate semialdehyde dehydrogenase (EC 1.2.1.26), at 426,416 to 427,996
_5978
Position (kb) 424
425
426
427 Strain fitness (log2 ratio) -2
-1
0
1
2 at 423.831 kb on + strand at 423.832 kb on - strand at 424.192 kb on + strand, within Pf1N1B4_5976 at 424.228 kb on + strand at 424.228 kb on + strand, within Pf1N1B4_5976 at 424.246 kb on + strand, within Pf1N1B4_5976 at 424.528 kb on + strand, within Pf1N1B4_5976 at 424.529 kb on - strand, within Pf1N1B4_5976 at 424.820 kb on - strand at 424.862 kb on - strand at 425.598 kb on + strand, within Pf1N1B4_5977 at 425.599 kb on - strand at 425.599 kb on - strand, within Pf1N1B4_5977 at 425.625 kb on + strand, within Pf1N1B4_5977 at 425.721 kb on + strand, within Pf1N1B4_5977 at 425.877 kb on + strand, within Pf1N1B4_5977 at 425.877 kb on + strand, within Pf1N1B4_5977 at 425.877 kb on + strand, within Pf1N1B4_5977 at 425.877 kb on + strand, within Pf1N1B4_5977 at 425.878 kb on - strand at 425.878 kb on - strand, within Pf1N1B4_5977 at 426.015 kb on + strand, within Pf1N1B4_5977 at 426.016 kb on - strand, within Pf1N1B4_5977 at 426.210 kb on + strand at 426.210 kb on + strand at 426.210 kb on + strand at 426.210 kb on + strand at 426.210 kb on + strand at 426.276 kb on + strand at 426.385 kb on + strand at 426.385 kb on + strand at 426.440 kb on + strand at 426.733 kb on + strand, within Pf1N1B4_5978 at 426.786 kb on - strand at 426.786 kb on - strand, within Pf1N1B4_5978 at 427.070 kb on + strand, within Pf1N1B4_5978 at 427.070 kb on + strand, within Pf1N1B4_5978 at 427.070 kb on + strand, within Pf1N1B4_5978 at 427.070 kb on + strand, within Pf1N1B4_5978 at 427.070 kb on + strand, within Pf1N1B4_5978 at 427.071 kb on - strand, within Pf1N1B4_5978 at 427.071 kb on - strand, within Pf1N1B4_5978 at 427.141 kb on - strand, within Pf1N1B4_5978
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 423,831 + +0.5 423,832 - +0.5 424,192 + Pf1N1B4_5976 0.41 +0.3 424,228 + -0.5 424,228 + Pf1N1B4_5976 0.45 -2.6 424,246 + Pf1N1B4_5976 0.47 -0.8 424,528 + Pf1N1B4_5976 0.77 +0.8 424,529 - Pf1N1B4_5976 0.78 +0.5 424,820 - -0.0 424,862 - +0.5 425,598 + Pf1N1B4_5977 0.57 +0.5 425,599 - -2.3 425,599 - Pf1N1B4_5977 0.57 +0.2 425,625 + Pf1N1B4_5977 0.59 -0.5 425,721 + Pf1N1B4_5977 0.65 +0.4 425,877 + Pf1N1B4_5977 0.77 +0.3 425,877 + Pf1N1B4_5977 0.77 +0.5 425,877 + Pf1N1B4_5977 0.77 -0.9 425,877 + Pf1N1B4_5977 0.77 -1.0 425,878 - -0.6 425,878 - Pf1N1B4_5977 0.77 -1.0 426,015 + Pf1N1B4_5977 0.86 +1.2 426,016 - Pf1N1B4_5977 0.87 +1.1 426,210 + +0.1 426,210 + -1.0 426,210 + -0.0 426,210 + +2.5 426,210 + -2.3 426,276 + -0.5 426,385 + +0.4 426,385 + -0.2 426,440 + +0.5 426,733 + Pf1N1B4_5978 0.20 +0.1 426,786 - +1.5 426,786 - Pf1N1B4_5978 0.23 -0.6 427,070 + Pf1N1B4_5978 0.41 +1.1 427,070 + Pf1N1B4_5978 0.41 -1.0 427,070 + Pf1N1B4_5978 0.41 -1.0 427,070 + Pf1N1B4_5978 0.41 +0.1 427,070 + Pf1N1B4_5978 0.41 -1.3 427,071 - Pf1N1B4_5978 0.41 -0.6 427,071 - Pf1N1B4_5978 0.41 +1.0 427,141 - Pf1N1B4_5978 0.46 -0.2
Or see this region's nucleotide sequence