Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5864 and Pf1N1B4_5865 are separated by 129 nucleotides Pf1N1B4_5865 and Pf1N1B4_5866 are separated by 159 nucleotides Pf1N1B4_5866 and Pf1N1B4_5867 are separated by 102 nucleotides
Pf1N1B4_5864 - Glycolate dehydrogenase (EC 1.1.99.14), iron-sulfur subunit GlcF, at 298,891 to 300,108
_5864
Pf1N1B4_5865 - FIG00960493: hypothetical protein, at 300,238 to 301,629
_5865
Pf1N1B4_5866 - ThiJ/PfpI family protein, at 301,789 to 302,466
_5866
Pf1N1B4_5867 - hypothetical protein, at 302,569 to 302,763
_5867
Position (kb) 300
301
302 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 299.531 kb on - strand, within Pf1N1B4_5864 at 299.692 kb on + strand, within Pf1N1B4_5864 at 299.693 kb on - strand, within Pf1N1B4_5864 at 299.814 kb on + strand at 299.849 kb on + strand, within Pf1N1B4_5864 at 299.929 kb on + strand at 300.060 kb on + strand at 300.061 kb on - strand at 300.328 kb on + strand at 300.532 kb on + strand, within Pf1N1B4_5865 at 300.533 kb on - strand, within Pf1N1B4_5865 at 300.766 kb on + strand at 300.767 kb on - strand, within Pf1N1B4_5865 at 300.909 kb on + strand, within Pf1N1B4_5865 at 301.234 kb on + strand, within Pf1N1B4_5865 at 301.234 kb on + strand, within Pf1N1B4_5865 at 301.393 kb on + strand, within Pf1N1B4_5865 at 301.394 kb on - strand, within Pf1N1B4_5865 at 301.394 kb on - strand, within Pf1N1B4_5865 at 301.417 kb on + strand, within Pf1N1B4_5865 at 301.418 kb on - strand, within Pf1N1B4_5865 at 301.489 kb on + strand, within Pf1N1B4_5865 at 301.562 kb on - strand at 301.641 kb on + strand at 301.878 kb on - strand, within Pf1N1B4_5866 at 302.186 kb on + strand, within Pf1N1B4_5866 at 302.227 kb on + strand, within Pf1N1B4_5866 at 302.228 kb on - strand, within Pf1N1B4_5866 at 302.372 kb on + strand, within Pf1N1B4_5866 at 302.373 kb on - strand, within Pf1N1B4_5866 at 302.373 kb on - strand, within Pf1N1B4_5866 at 302.373 kb on - strand, within Pf1N1B4_5866 at 302.373 kb on - strand, within Pf1N1B4_5866 at 302.376 kb on - strand, within Pf1N1B4_5866 at 302.376 kb on - strand, within Pf1N1B4_5866
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 299,531 - Pf1N1B4_5864 0.53 +0.3 299,692 + Pf1N1B4_5864 0.66 +0.5 299,693 - Pf1N1B4_5864 0.66 -0.5 299,814 + -0.5 299,849 + Pf1N1B4_5864 0.79 +1.0 299,929 + +0.5 300,060 + -0.9 300,061 - -1.0 300,328 + -1.0 300,532 + Pf1N1B4_5865 0.21 +0.2 300,533 - Pf1N1B4_5865 0.21 -0.1 300,766 + +0.4 300,767 - Pf1N1B4_5865 0.38 -0.7 300,909 + Pf1N1B4_5865 0.48 -0.2 301,234 + Pf1N1B4_5865 0.72 -0.9 301,234 + Pf1N1B4_5865 0.72 -1.7 301,393 + Pf1N1B4_5865 0.83 -1.6 301,394 - Pf1N1B4_5865 0.83 -1.8 301,394 - Pf1N1B4_5865 0.83 -0.0 301,417 + Pf1N1B4_5865 0.85 -1.0 301,418 - Pf1N1B4_5865 0.85 -0.5 301,489 + Pf1N1B4_5865 0.90 -2.3 301,562 - -1.0 301,641 + +2.9 301,878 - Pf1N1B4_5866 0.13 +0.1 302,186 + Pf1N1B4_5866 0.59 -0.9 302,227 + Pf1N1B4_5866 0.65 -1.0 302,228 - Pf1N1B4_5866 0.65 -0.6 302,372 + Pf1N1B4_5866 0.86 -0.9 302,373 - Pf1N1B4_5866 0.86 -2.8 302,373 - Pf1N1B4_5866 0.86 -0.0 302,373 - Pf1N1B4_5866 0.86 +0.1 302,373 - Pf1N1B4_5866 0.86 +0.9 302,376 - Pf1N1B4_5866 0.87 -0.3 302,376 - Pf1N1B4_5866 0.87 -1.5
Or see this region's nucleotide sequence