Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5768

Experiment: LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5767 and Pf1N1B4_5768 are separated by 1 nucleotidesPf1N1B4_5768 and Pf1N1B4_5769 overlap by 4 nucleotidesPf1N1B4_5769 and Pf1N1B4_5770 overlap by 8 nucleotidesPf1N1B4_5770 and Pf1N1B4_5771 overlap by 26 nucleotides Pf1N1B4_5767 - Heme d1 biosynthesis protein NirF, at 205,503 to 206,681 _5767 Pf1N1B4_5768 - Heme d1 biosynthesis protein NirD, at 206,683 to 207,141 _5768 Pf1N1B4_5769 - Heme d1 biosynthesis protein NirL, at 207,138 to 207,653 _5769 Pf1N1B4_5770 - Heme d1 biosynthesis protein NirG, at 207,646 to 208,089 _5770 Pf1N1B4_5771 - Heme d1 biosynthesis protein NirH, at 208,064 to 208,579 _5771 Position (kb) 206 207 208Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 205.725 kb on + strand, within Pf1N1B4_5767at 205.725 kb on + strand, within Pf1N1B4_5767at 205.725 kb on + strand, within Pf1N1B4_5767at 205.725 kb on + strandat 205.726 kb on - strand, within Pf1N1B4_5767at 205.726 kb on - strand, within Pf1N1B4_5767at 205.726 kb on - strand, within Pf1N1B4_5767at 205.726 kb on - strand, within Pf1N1B4_5767at 205.726 kb on - strand, within Pf1N1B4_5767at 205.726 kb on - strand, within Pf1N1B4_5767at 205.796 kb on - strand, within Pf1N1B4_5767at 205.860 kb on + strand, within Pf1N1B4_5767at 205.861 kb on - strand, within Pf1N1B4_5767at 205.861 kb on - strand, within Pf1N1B4_5767at 205.861 kb on - strand, within Pf1N1B4_5767at 205.861 kb on - strandat 205.861 kb on - strand, within Pf1N1B4_5767at 206.094 kb on + strand, within Pf1N1B4_5767at 206.095 kb on - strand, within Pf1N1B4_5767at 206.127 kb on + strand, within Pf1N1B4_5767at 206.127 kb on + strand, within Pf1N1B4_5767at 206.127 kb on + strand, within Pf1N1B4_5767at 206.127 kb on + strand, within Pf1N1B4_5767at 206.128 kb on - strand, within Pf1N1B4_5767at 206.756 kb on - strand, within Pf1N1B4_5768at 206.756 kb on - strand, within Pf1N1B4_5768at 206.964 kb on + strand, within Pf1N1B4_5768at 206.965 kb on - strand, within Pf1N1B4_5768at 206.968 kb on + strandat 206.968 kb on + strand, within Pf1N1B4_5768at 206.969 kb on - strand, within Pf1N1B4_5768at 206.987 kb on - strand, within Pf1N1B4_5768at 207.344 kb on + strand, within Pf1N1B4_5769at 207.344 kb on + strand, within Pf1N1B4_5769at 207.439 kb on - strand, within Pf1N1B4_5769at 207.472 kb on - strand, within Pf1N1B4_5769at 207.929 kb on - strand, within Pf1N1B4_5770at 207.929 kb on - strand, within Pf1N1B4_5770at 208.060 kb on + strandat 208.060 kb on + strandat 208.061 kb on - strandat 208.076 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB
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205,725 + Pf1N1B4_5767 0.19 +0.0
205,725 + Pf1N1B4_5767 0.19 +0.1
205,725 + Pf1N1B4_5767 0.19 -0.5
205,725 + -0.5
205,726 - Pf1N1B4_5767 0.19 -0.7
205,726 - Pf1N1B4_5767 0.19 -0.5
205,726 - Pf1N1B4_5767 0.19 -1.3
205,726 - Pf1N1B4_5767 0.19 -0.5
205,726 - Pf1N1B4_5767 0.19 +1.2
205,726 - Pf1N1B4_5767 0.19 -2.8
205,796 - Pf1N1B4_5767 0.25 +0.1
205,860 + Pf1N1B4_5767 0.30 -0.6
205,861 - Pf1N1B4_5767 0.30 +2.1
205,861 - Pf1N1B4_5767 0.30 -1.5
205,861 - Pf1N1B4_5767 0.30 -0.0
205,861 - +1.5
205,861 - Pf1N1B4_5767 0.30 +0.5
206,094 + Pf1N1B4_5767 0.50 -0.8
206,095 - Pf1N1B4_5767 0.50 +0.7
206,127 + Pf1N1B4_5767 0.53 +0.1
206,127 + Pf1N1B4_5767 0.53 -2.2
206,127 + Pf1N1B4_5767 0.53 +0.9
206,127 + Pf1N1B4_5767 0.53 +0.0
206,128 - Pf1N1B4_5767 0.53 -0.1
206,756 - Pf1N1B4_5768 0.16 -0.5
206,756 - Pf1N1B4_5768 0.16 -1.5
206,964 + Pf1N1B4_5768 0.61 -1.0
206,965 - Pf1N1B4_5768 0.61 -0.5
206,968 + -1.8
206,968 + Pf1N1B4_5768 0.62 -1.1
206,969 - Pf1N1B4_5768 0.62 -1.1
206,987 - Pf1N1B4_5768 0.66 -0.9
207,344 + Pf1N1B4_5769 0.40 +0.7
207,344 + Pf1N1B4_5769 0.40 +0.5
207,439 - Pf1N1B4_5769 0.58 -0.8
207,472 - Pf1N1B4_5769 0.65 -2.5
207,929 - Pf1N1B4_5770 0.64 +0.1
207,929 - Pf1N1B4_5770 0.64 -0.8
208,060 + -0.6
208,060 + -0.0
208,061 - -0.5
208,076 - -0.1

Or see this region's nucleotide sequence