Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5708 and Pf1N1B4_5709 are separated by 355 nucleotides Pf1N1B4_5709 and Pf1N1B4_5710 are separated by 22 nucleotides Pf1N1B4_5710 and Pf1N1B4_5711 are separated by 92 nucleotides
Pf1N1B4_5708 - Transcriptional regulator, TetR family, at 146,847 to 147,461
_5708
Pf1N1B4_5709 - 2,4-dienoyl-CoA reductase [NADPH] (EC 1.3.1.34), at 147,817 to 149,052
_5709
Pf1N1B4_5710 - Transcriptional regulator, TetR family, at 149,075 to 149,629
_5710
Pf1N1B4_5711 - Salicylate hydroxylase (EC 1.14.13.1), at 149,722 to 151,380
_5711
Position (kb)
147
148
149
150 Strain fitness (log2 ratio)
-2
-1
0
1 at 147.330 kb on - strand, within Pf1N1B4_5708 at 147.332 kb on - strand, within Pf1N1B4_5708 at 147.332 kb on - strand, within Pf1N1B4_5708 at 147.480 kb on - strand at 147.567 kb on + strand at 147.567 kb on + strand at 147.567 kb on + strand at 147.590 kb on + strand at 147.591 kb on - strand at 147.591 kb on - strand at 147.591 kb on - strand at 147.623 kb on + strand at 147.747 kb on - strand at 148.148 kb on + strand, within Pf1N1B4_5709 at 148.148 kb on + strand, within Pf1N1B4_5709 at 148.149 kb on - strand, within Pf1N1B4_5709 at 148.278 kb on + strand, within Pf1N1B4_5709 at 148.604 kb on - strand, within Pf1N1B4_5709 at 148.664 kb on - strand, within Pf1N1B4_5709 at 149.026 kb on + strand at 149.121 kb on - strand at 149.175 kb on - strand, within Pf1N1B4_5710 at 149.219 kb on + strand, within Pf1N1B4_5710 at 149.219 kb on + strand, within Pf1N1B4_5710 at 149.526 kb on + strand, within Pf1N1B4_5710 at 149.527 kb on - strand, within Pf1N1B4_5710 at 149.732 kb on - strand at 149.856 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 147,330 - Pf1N1B4_5708 0.79 +0.5 147,332 - Pf1N1B4_5708 0.79 -0.6 147,332 - Pf1N1B4_5708 0.79 -0.5 147,480 - -0.4 147,567 + -1.3 147,567 + -0.5 147,567 + -0.5 147,590 + +0.5 147,591 - -0.0 147,591 - -1.5 147,591 - -0.1 147,623 + -1.1 147,747 - -2.2 148,148 + Pf1N1B4_5709 0.27 -2.1 148,148 + Pf1N1B4_5709 0.27 -1.3 148,149 - Pf1N1B4_5709 0.27 -0.5 148,278 + Pf1N1B4_5709 0.37 -1.0 148,604 - Pf1N1B4_5709 0.64 -0.1 148,664 - Pf1N1B4_5709 0.69 -1.1 149,026 + +1.1 149,121 - -0.1 149,175 - Pf1N1B4_5710 0.18 -0.3 149,219 + Pf1N1B4_5710 0.26 -1.5 149,219 + Pf1N1B4_5710 0.26 -0.4 149,526 + Pf1N1B4_5710 0.81 +1.0 149,527 - Pf1N1B4_5710 0.81 -0.5 149,732 - +0.2 149,856 + -0.8
Or see this region's nucleotide sequence