Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5684 and Pf1N1B4_5685 are separated by 7 nucleotides Pf1N1B4_5685 and Pf1N1B4_5686 are separated by 2 nucleotides Pf1N1B4_5686 and Pf1N1B4_5687 are separated by 132 nucleotides Pf1N1B4_5687 and Pf1N1B4_5688 are separated by 57 nucleotides Pf1N1B4_5688 and Pf1N1B4_5689 are separated by 11 nucleotides
Pf1N1B4_5684 - Glyceraldehyde-3-phosphate dehydrogenase, putative, at 123,711 to 125,336
_5684
Pf1N1B4_5685 - Permease of the drug/metabolite transporter (DMT) superfamily, at 125,344 to 125,835
_5685
Pf1N1B4_5686 - Permease of the drug/metabolite transporter (DMT) superfamily, at 125,838 to 126,239
_5686
Pf1N1B4_5687 - Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system, at 126,372 to 126,809
_5687
Pf1N1B4_5688 - hypothetical protein, at 126,867 to 127,196
_5688
Pf1N1B4_5689 - Lactoylglutathione lyase, at 127,208 to 127,600
_5689
Position (kb)
125
126
127 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 125.016 kb on + strand, within Pf1N1B4_5684 at 125.017 kb on - strand, within Pf1N1B4_5684 at 125.158 kb on - strand, within Pf1N1B4_5684 at 125.326 kb on - strand at 125.558 kb on + strand, within Pf1N1B4_5685 at 125.558 kb on + strand, within Pf1N1B4_5685 at 125.558 kb on + strand, within Pf1N1B4_5685 at 125.558 kb on + strand at 125.558 kb on + strand, within Pf1N1B4_5685 at 125.559 kb on - strand at 125.559 kb on - strand, within Pf1N1B4_5685 at 125.559 kb on - strand at 125.559 kb on - strand, within Pf1N1B4_5685 at 125.784 kb on - strand, within Pf1N1B4_5685 at 125.935 kb on + strand, within Pf1N1B4_5686 at 125.936 kb on - strand, within Pf1N1B4_5686 at 126.222 kb on + strand at 126.223 kb on - strand at 126.250 kb on + strand at 126.251 kb on - strand at 126.522 kb on + strand, within Pf1N1B4_5687 at 126.523 kb on - strand, within Pf1N1B4_5687 at 126.834 kb on - strand at 126.901 kb on + strand, within Pf1N1B4_5688 at 127.005 kb on + strand, within Pf1N1B4_5688 at 127.005 kb on + strand, within Pf1N1B4_5688 at 127.005 kb on + strand, within Pf1N1B4_5688 at 127.006 kb on - strand at 127.020 kb on + strand, within Pf1N1B4_5688 at 127.068 kb on + strand, within Pf1N1B4_5688 at 127.069 kb on - strand, within Pf1N1B4_5688 at 127.074 kb on + strand at 127.074 kb on + strand, within Pf1N1B4_5688 at 127.075 kb on - strand, within Pf1N1B4_5688 at 127.075 kb on - strand, within Pf1N1B4_5688 at 127.096 kb on + strand, within Pf1N1B4_5688 at 127.097 kb on - strand, within Pf1N1B4_5688 at 127.109 kb on - strand, within Pf1N1B4_5688 at 127.155 kb on + strand, within Pf1N1B4_5688
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 125,016 + Pf1N1B4_5684 0.80 -0.5 125,017 - Pf1N1B4_5684 0.80 +0.5 125,158 - Pf1N1B4_5684 0.89 +0.5 125,326 - -0.3 125,558 + Pf1N1B4_5685 0.43 -1.3 125,558 + Pf1N1B4_5685 0.43 -1.0 125,558 + Pf1N1B4_5685 0.43 -1.6 125,558 + +1.5 125,558 + Pf1N1B4_5685 0.43 +0.1 125,559 - -1.1 125,559 - Pf1N1B4_5685 0.44 -0.5 125,559 - -0.1 125,559 - Pf1N1B4_5685 0.44 -1.8 125,784 - Pf1N1B4_5685 0.89 +1.1 125,935 + Pf1N1B4_5686 0.24 -0.3 125,936 - Pf1N1B4_5686 0.24 -0.5 126,222 + -1.1 126,223 - +1.2 126,250 + +0.0 126,251 - -0.3 126,522 + Pf1N1B4_5687 0.34 -0.5 126,523 - Pf1N1B4_5687 0.34 -0.8 126,834 - -1.1 126,901 + Pf1N1B4_5688 0.10 +1.5 127,005 + Pf1N1B4_5688 0.42 -0.5 127,005 + Pf1N1B4_5688 0.42 -1.1 127,005 + Pf1N1B4_5688 0.42 +2.7 127,006 - -0.5 127,020 + Pf1N1B4_5688 0.46 +0.5 127,068 + Pf1N1B4_5688 0.61 -0.3 127,069 - Pf1N1B4_5688 0.61 -0.8 127,074 + -0.3 127,074 + Pf1N1B4_5688 0.63 -0.7 127,075 - Pf1N1B4_5688 0.63 -0.3 127,075 - Pf1N1B4_5688 0.63 -0.1 127,096 + Pf1N1B4_5688 0.69 -1.3 127,097 - Pf1N1B4_5688 0.70 -0.7 127,109 - Pf1N1B4_5688 0.73 -0.9 127,155 + Pf1N1B4_5688 0.87 +0.5
Or see this region's nucleotide sequence