Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5673 and Pf1N1B4_5674 overlap by 4 nucleotides Pf1N1B4_5674 and Pf1N1B4_5675 are separated by 150 nucleotides Pf1N1B4_5675 and Pf1N1B4_5676 are separated by 108 nucleotides Pf1N1B4_5676 and Pf1N1B4_5677 are separated by 218 nucleotides
Pf1N1B4_5673 - Sterol desaturase, at 110,665 to 111,807
_5673
Pf1N1B4_5674 - Strictosidine synthase precursor (EC 4.3.3.2), at 111,804 to 112,892
_5674
Pf1N1B4_5675 - hypothetical protein, at 113,043 to 113,303
_5675
Pf1N1B4_5676 - hypothetical protein, at 113,412 to 113,654
_5676
Pf1N1B4_5677 - putative exported protein, at 113,873 to 114,394
_5677
Position (kb)
111
112
113 Strain fitness (log2 ratio)
-2
-1
0
1 at 110.805 kb on - strand, within Pf1N1B4_5673 at 110.918 kb on - strand, within Pf1N1B4_5673 at 110.971 kb on + strand, within Pf1N1B4_5673 at 110.972 kb on - strand, within Pf1N1B4_5673 at 111.131 kb on - strand at 111.284 kb on - strand, within Pf1N1B4_5673 at 111.476 kb on - strand, within Pf1N1B4_5673 at 111.616 kb on + strand, within Pf1N1B4_5673 at 111.683 kb on + strand, within Pf1N1B4_5673 at 111.832 kb on + strand at 111.832 kb on + strand at 111.832 kb on + strand at 111.833 kb on - strand at 111.833 kb on - strand at 111.874 kb on + strand at 111.943 kb on - strand, within Pf1N1B4_5674 at 112.064 kb on + strand at 112.614 kb on + strand, within Pf1N1B4_5674 at 112.614 kb on + strand, within Pf1N1B4_5674 at 112.615 kb on - strand at 112.615 kb on - strand, within Pf1N1B4_5674 at 112.663 kb on + strand, within Pf1N1B4_5674 at 112.663 kb on + strand, within Pf1N1B4_5674 at 112.788 kb on + strand at 112.788 kb on + strand at 112.788 kb on + strand at 112.788 kb on + strand at 112.815 kb on + strand at 112.816 kb on - strand at 112.830 kb on + strand at 112.830 kb on + strand at 112.890 kb on + strand at 112.891 kb on - strand at 112.891 kb on - strand at 113.055 kb on + strand at 113.056 kb on - strand at 113.056 kb on - strand at 113.056 kb on - strand at 113.142 kb on + strand, within Pf1N1B4_5675 at 113.143 kb on - strand, within Pf1N1B4_5675 at 113.143 kb on - strand, within Pf1N1B4_5675 at 113.228 kb on - strand, within Pf1N1B4_5675 at 113.356 kb on - strand at 113.360 kb on - strand at 113.406 kb on - strand at 113.551 kb on + strand, within Pf1N1B4_5676 at 113.552 kb on - strand, within Pf1N1B4_5676 at 113.848 kb on + strand at 113.849 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 110,805 - Pf1N1B4_5673 0.12 -0.8 110,918 - Pf1N1B4_5673 0.22 +0.1 110,971 + Pf1N1B4_5673 0.27 -0.7 110,972 - Pf1N1B4_5673 0.27 -1.1 111,131 - -0.1 111,284 - Pf1N1B4_5673 0.54 -0.4 111,476 - Pf1N1B4_5673 0.71 +0.3 111,616 + Pf1N1B4_5673 0.83 -1.1 111,683 + Pf1N1B4_5673 0.89 -0.3 111,832 + -1.5 111,832 + +0.0 111,832 + +0.5 111,833 - +0.5 111,833 - -1.3 111,874 + -0.1 111,943 - Pf1N1B4_5674 0.13 +0.0 112,064 + +0.5 112,614 + Pf1N1B4_5674 0.74 +0.2 112,614 + Pf1N1B4_5674 0.74 -0.3 112,615 - +1.5 112,615 - Pf1N1B4_5674 0.74 +1.0 112,663 + Pf1N1B4_5674 0.79 +0.2 112,663 + Pf1N1B4_5674 0.79 +1.1 112,788 + -0.1 112,788 + +0.5 112,788 + -0.7 112,788 + -2.1 112,815 + -0.1 112,816 - -1.1 112,830 + +0.3 112,830 + +0.8 112,890 + -0.5 112,891 - -0.8 112,891 - -0.5 113,055 + -0.1 113,056 - -2.1 113,056 - +0.4 113,056 - -0.5 113,142 + Pf1N1B4_5675 0.38 +0.8 113,143 - Pf1N1B4_5675 0.38 -0.5 113,143 - Pf1N1B4_5675 0.38 -0.3 113,228 - Pf1N1B4_5675 0.71 -1.5 113,356 - +0.3 113,360 - -2.5 113,406 - -0.7 113,551 + Pf1N1B4_5676 0.57 +0.2 113,552 - Pf1N1B4_5676 0.58 -0.6 113,848 + +0.2 113,849 - -0.1
Or see this region's nucleotide sequence