Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4965 and Pf1N1B4_4966 are separated by 280 nucleotides Pf1N1B4_4966 and Pf1N1B4_4967 are separated by 229 nucleotides Pf1N1B4_4967 and Pf1N1B4_4968 are separated by 170 nucleotides
Pf1N1B4_4965 - Tricarboxylate transport membrane protein TctA, at 5,505,772 to 5,507,280
_4965
Pf1N1B4_4966 - Transcriptional regulator, LysR family, at 5,507,561 to 5,508,454
_4966
Pf1N1B4_4967 - Transcriptional regulator, at 5,508,684 to 5,509,088
_4967
Pf1N1B4_4968 - 4-carboxymuconolactone decarboxylase (EC 4.1.1.44), at 5,509,259 to 5,510,083
_4968
Position (kb)
5507
5508
5509 Strain fitness (log2 ratio)
-2
-1
0
1 at 5506.606 kb on + strand at 5506.607 kb on - strand, within Pf1N1B4_4965 at 5506.798 kb on + strand, within Pf1N1B4_4965 at 5506.799 kb on - strand, within Pf1N1B4_4965 at 5506.799 kb on - strand, within Pf1N1B4_4965 at 5506.870 kb on + strand, within Pf1N1B4_4965 at 5506.870 kb on + strand, within Pf1N1B4_4965 at 5506.871 kb on - strand, within Pf1N1B4_4965 at 5506.871 kb on - strand, within Pf1N1B4_4965 at 5506.874 kb on + strand, within Pf1N1B4_4965 at 5506.874 kb on + strand, within Pf1N1B4_4965 at 5506.875 kb on - strand, within Pf1N1B4_4965 at 5506.875 kb on - strand, within Pf1N1B4_4965 at 5506.951 kb on + strand, within Pf1N1B4_4965 at 5506.993 kb on + strand, within Pf1N1B4_4965 at 5507.247 kb on + strand at 5507.345 kb on - strand at 5507.632 kb on - strand at 5507.769 kb on + strand, within Pf1N1B4_4966 at 5507.770 kb on - strand, within Pf1N1B4_4966 at 5507.844 kb on + strand, within Pf1N1B4_4966 at 5507.844 kb on + strand, within Pf1N1B4_4966 at 5507.845 kb on - strand, within Pf1N1B4_4966 at 5507.857 kb on + strand, within Pf1N1B4_4966 at 5507.860 kb on + strand, within Pf1N1B4_4966 at 5507.993 kb on - strand, within Pf1N1B4_4966 at 5508.003 kb on - strand, within Pf1N1B4_4966 at 5508.343 kb on + strand, within Pf1N1B4_4966 at 5508.428 kb on + strand at 5508.428 kb on + strand at 5508.471 kb on + strand at 5508.475 kb on + strand at 5508.475 kb on + strand at 5508.475 kb on + strand at 5508.475 kb on + strand at 5508.476 kb on - strand at 5508.476 kb on - strand at 5508.476 kb on - strand at 5508.489 kb on - strand at 5508.518 kb on + strand at 5508.518 kb on + strand at 5508.739 kb on - strand, within Pf1N1B4_4967 at 5509.031 kb on + strand, within Pf1N1B4_4967 at 5509.072 kb on - strand at 5509.076 kb on + strand at 5509.336 kb on - strand at 5509.336 kb on - strand at 5509.336 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 5,506,606 + +0.8 5,506,607 - Pf1N1B4_4965 0.55 +0.8 5,506,798 + Pf1N1B4_4965 0.68 -0.0 5,506,799 - Pf1N1B4_4965 0.68 +0.4 5,506,799 - Pf1N1B4_4965 0.68 -1.4 5,506,870 + Pf1N1B4_4965 0.73 -2.4 5,506,870 + Pf1N1B4_4965 0.73 +1.3 5,506,871 - Pf1N1B4_4965 0.73 -0.3 5,506,871 - Pf1N1B4_4965 0.73 -1.3 5,506,874 + Pf1N1B4_4965 0.73 -0.0 5,506,874 + Pf1N1B4_4965 0.73 +0.4 5,506,875 - Pf1N1B4_4965 0.73 -1.2 5,506,875 - Pf1N1B4_4965 0.73 -0.9 5,506,951 + Pf1N1B4_4965 0.78 -2.2 5,506,993 + Pf1N1B4_4965 0.81 -0.5 5,507,247 + -1.2 5,507,345 - -1.6 5,507,632 - +0.4 5,507,769 + Pf1N1B4_4966 0.23 -0.6 5,507,770 - Pf1N1B4_4966 0.23 -0.7 5,507,844 + Pf1N1B4_4966 0.32 +1.2 5,507,844 + Pf1N1B4_4966 0.32 -0.6 5,507,845 - Pf1N1B4_4966 0.32 -0.6 5,507,857 + Pf1N1B4_4966 0.33 -1.9 5,507,860 + Pf1N1B4_4966 0.33 -0.6 5,507,993 - Pf1N1B4_4966 0.48 -1.6 5,508,003 - Pf1N1B4_4966 0.49 -0.9 5,508,343 + Pf1N1B4_4966 0.87 -1.2 5,508,428 + +0.2 5,508,428 + +0.4 5,508,471 + -1.4 5,508,475 + -0.6 5,508,475 + +0.1 5,508,475 + -0.6 5,508,475 + +0.4 5,508,476 - -2.2 5,508,476 - -1.0 5,508,476 - -0.8 5,508,489 - -0.9 5,508,518 + -1.0 5,508,518 + +1.0 5,508,739 - Pf1N1B4_4967 0.14 -2.2 5,509,031 + Pf1N1B4_4967 0.86 -0.3 5,509,072 - +0.6 5,509,076 + -1.6 5,509,336 - +1.5 5,509,336 - -0.3 5,509,336 - +0.4
Or see this region's nucleotide sequence