Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4776 and Pf1N1B4_4777 are separated by 325 nucleotides Pf1N1B4_4777 and Pf1N1B4_4778 are separated by 6 nucleotides Pf1N1B4_4778 and Pf1N1B4_4779 overlap by 33 nucleotides
Pf1N1B4_4776 - Nicotinamidase (EC 3.5.1.19), at 5,278,108 to 5,278,746
_4776
Pf1N1B4_4777 - Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17), at 5,279,072 to 5,279,884
_4777
Pf1N1B4_4778 - NADH pyrophosphatase (EC 3.6.1.22), at 5,279,891 to 5,280,721
_4778
Pf1N1B4_4779 - Membrane protein, putative, at 5,280,689 to 5,281,468
_4779
Position (kb) 5279
5280 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5278.127 kb on - strand at 5278.307 kb on + strand, within Pf1N1B4_4776 at 5278.307 kb on + strand, within Pf1N1B4_4776 at 5278.308 kb on - strand, within Pf1N1B4_4776 at 5278.308 kb on - strand, within Pf1N1B4_4776 at 5278.308 kb on - strand, within Pf1N1B4_4776 at 5278.308 kb on - strand, within Pf1N1B4_4776 at 5278.388 kb on + strand, within Pf1N1B4_4776 at 5278.477 kb on - strand, within Pf1N1B4_4776 at 5278.539 kb on - strand at 5278.705 kb on + strand at 5278.705 kb on + strand at 5278.706 kb on - strand at 5278.706 kb on - strand at 5278.783 kb on - strand at 5278.783 kb on - strand at 5278.788 kb on + strand at 5278.788 kb on + strand at 5278.789 kb on - strand at 5278.899 kb on + strand at 5279.031 kb on + strand at 5279.032 kb on - strand at 5279.032 kb on - strand at 5279.045 kb on + strand at 5279.046 kb on - strand at 5279.046 kb on - strand at 5279.046 kb on - strand at 5279.082 kb on - strand at 5279.530 kb on - strand, within Pf1N1B4_4777 at 5279.530 kb on - strand, within Pf1N1B4_4777 at 5279.588 kb on + strand, within Pf1N1B4_4777 at 5279.649 kb on - strand, within Pf1N1B4_4777 at 5279.750 kb on + strand, within Pf1N1B4_4777 at 5279.787 kb on - strand, within Pf1N1B4_4777 at 5279.851 kb on + strand at 5279.851 kb on + strand at 5279.852 kb on - strand at 5280.167 kb on + strand, within Pf1N1B4_4778 at 5280.168 kb on - strand, within Pf1N1B4_4778 at 5280.168 kb on - strand at 5280.275 kb on + strand, within Pf1N1B4_4778 at 5280.275 kb on + strand, within Pf1N1B4_4778 at 5280.276 kb on - strand at 5280.294 kb on + strand, within Pf1N1B4_4778 at 5280.294 kb on + strand, within Pf1N1B4_4778 at 5280.295 kb on - strand, within Pf1N1B4_4778 at 5280.295 kb on - strand, within Pf1N1B4_4778 at 5280.393 kb on - strand, within Pf1N1B4_4778 at 5280.398 kb on - strand, within Pf1N1B4_4778 at 5280.398 kb on - strand, within Pf1N1B4_4778 at 5280.494 kb on + strand, within Pf1N1B4_4778 at 5280.494 kb on + strand at 5280.494 kb on + strand at 5280.494 kb on + strand, within Pf1N1B4_4778 at 5280.494 kb on + strand, within Pf1N1B4_4778 at 5280.674 kb on + strand at 5280.674 kb on + strand at 5280.674 kb on + strand at 5280.675 kb on - strand at 5280.872 kb on + strand, within Pf1N1B4_4779
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 5,278,127 - +0.2 5,278,307 + Pf1N1B4_4776 0.31 -1.6 5,278,307 + Pf1N1B4_4776 0.31 -0.9 5,278,308 - Pf1N1B4_4776 0.31 +0.9 5,278,308 - Pf1N1B4_4776 0.31 -0.5 5,278,308 - Pf1N1B4_4776 0.31 -1.7 5,278,308 - Pf1N1B4_4776 0.31 +2.0 5,278,388 + Pf1N1B4_4776 0.44 -1.1 5,278,477 - Pf1N1B4_4776 0.58 -2.6 5,278,539 - +1.0 5,278,705 + -0.2 5,278,705 + +0.4 5,278,706 - -1.6 5,278,706 - +0.8 5,278,783 - -1.6 5,278,783 - -0.8 5,278,788 + -1.2 5,278,788 + -1.9 5,278,789 - +0.4 5,278,899 + +0.6 5,279,031 + +0.3 5,279,032 - -0.6 5,279,032 - +0.4 5,279,045 + -1.6 5,279,046 - -0.7 5,279,046 - +1.0 5,279,046 - +0.6 5,279,082 - -1.6 5,279,530 - Pf1N1B4_4777 0.56 -2.2 5,279,530 - Pf1N1B4_4777 0.56 -0.8 5,279,588 + Pf1N1B4_4777 0.63 -1.9 5,279,649 - Pf1N1B4_4777 0.71 -1.6 5,279,750 + Pf1N1B4_4777 0.83 -1.2 5,279,787 - Pf1N1B4_4777 0.88 +0.7 5,279,851 + -0.9 5,279,851 + -0.3 5,279,852 - -0.2 5,280,167 + Pf1N1B4_4778 0.33 -1.0 5,280,168 - Pf1N1B4_4778 0.33 +0.4 5,280,168 - -0.6 5,280,275 + Pf1N1B4_4778 0.46 +0.1 5,280,275 + Pf1N1B4_4778 0.46 +0.6 5,280,276 - +0.4 5,280,294 + Pf1N1B4_4778 0.48 +1.1 5,280,294 + Pf1N1B4_4778 0.48 +0.1 5,280,295 - Pf1N1B4_4778 0.49 -0.9 5,280,295 - Pf1N1B4_4778 0.49 -0.6 5,280,393 - Pf1N1B4_4778 0.60 +0.4 5,280,398 - Pf1N1B4_4778 0.61 -0.6 5,280,398 - Pf1N1B4_4778 0.61 -1.6 5,280,494 + Pf1N1B4_4778 0.73 +0.1 5,280,494 + +0.4 5,280,494 + +1.6 5,280,494 + Pf1N1B4_4778 0.73 -1.4 5,280,494 + Pf1N1B4_4778 0.73 +0.4 5,280,674 + +0.4 5,280,674 + +1.4 5,280,674 + -0.7 5,280,675 - -0.1 5,280,872 + Pf1N1B4_4779 0.23 -0.6
Or see this region's nucleotide sequence