Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4629 and Pf1N1B4_4630 overlap by 4 nucleotides Pf1N1B4_4630 and Pf1N1B4_4631 are separated by 118 nucleotides Pf1N1B4_4631 and Pf1N1B4_4632 are separated by 108 nucleotides Pf1N1B4_4632 and Pf1N1B4_4633 overlap by 4 nucleotides
Pf1N1B4_4629 - hypothetical protein, at 5,104,642 to 5,104,854
_4629
Pf1N1B4_4630 - hypothetical protein, at 5,104,851 to 5,105,108
_4630
Pf1N1B4_4631 - Glutathione S-transferase family protein, at 5,105,227 to 5,105,703
_4631
Pf1N1B4_4632 - Exonuclease SbcC, at 5,105,812 to 5,109,453
_4632
Pf1N1B4_4633 - Exonuclease SbcD, at 5,109,450 to 5,110,706
_4633
Position (kb)
5105
5106
5107
5108
5109
5110 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5105.170 kb on - strand at 5105.170 kb on - strand at 5105.170 kb on - strand at 5105.170 kb on - strand at 5105.170 kb on - strand at 5105.170 kb on - strand at 5105.173 kb on + strand at 5105.240 kb on - strand at 5105.297 kb on + strand, within Pf1N1B4_4631 at 5105.298 kb on - strand, within Pf1N1B4_4631 at 5105.493 kb on - strand, within Pf1N1B4_4631 at 5106.237 kb on - strand, within Pf1N1B4_4632 at 5106.237 kb on - strand, within Pf1N1B4_4632 at 5106.968 kb on + strand, within Pf1N1B4_4632 at 5107.982 kb on + strand, within Pf1N1B4_4632 at 5108.864 kb on + strand, within Pf1N1B4_4632 at 5108.864 kb on + strand, within Pf1N1B4_4632 at 5108.865 kb on - strand, within Pf1N1B4_4632 at 5109.173 kb on + strand at 5109.474 kb on + strand at 5109.474 kb on + strand at 5109.533 kb on - strand at 5110.028 kb on - strand, within Pf1N1B4_4633 at 5110.222 kb on + strand, within Pf1N1B4_4633 at 5110.222 kb on + strand, within Pf1N1B4_4633 at 5110.223 kb on - strand, within Pf1N1B4_4633 at 5110.227 kb on - strand, within Pf1N1B4_4633
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 5,105,170 - +2.0 5,105,170 - -0.9 5,105,170 - +0.0 5,105,170 - -1.5 5,105,170 - -0.7 5,105,170 - -0.5 5,105,173 + -1.9 5,105,240 - +0.5 5,105,297 + Pf1N1B4_4631 0.15 -1.5 5,105,298 - Pf1N1B4_4631 0.15 +0.5 5,105,493 - Pf1N1B4_4631 0.56 +0.5 5,106,237 - Pf1N1B4_4632 0.12 -0.6 5,106,237 - Pf1N1B4_4632 0.12 -1.9 5,106,968 + Pf1N1B4_4632 0.32 +0.5 5,107,982 + Pf1N1B4_4632 0.60 -1.1 5,108,864 + Pf1N1B4_4632 0.84 +0.7 5,108,864 + Pf1N1B4_4632 0.84 -0.9 5,108,865 - Pf1N1B4_4632 0.84 -0.9 5,109,173 + -0.2 5,109,474 + -0.4 5,109,474 + -0.5 5,109,533 - +0.5 5,110,028 - Pf1N1B4_4633 0.46 +0.5 5,110,222 + Pf1N1B4_4633 0.61 -2.1 5,110,222 + Pf1N1B4_4633 0.61 +0.5 5,110,223 - Pf1N1B4_4633 0.61 +0.1 5,110,227 - Pf1N1B4_4633 0.62 -0.3
Or see this region's nucleotide sequence