Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4533

Experiment: LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4532 and Pf1N1B4_4533 are separated by 2 nucleotidesPf1N1B4_4533 and Pf1N1B4_4534 overlap by 37 nucleotides Pf1N1B4_4532 - ClpB protein, at 4,997,929 to 5,000,472 _4532 Pf1N1B4_4533 - Uncharacterized protein ImpH/VasB, at 5,000,475 to 5,001,500 _4533 Pf1N1B4_4534 - Protein ImpG/VasA, at 5,001,464 to 5,003,257 _4534 Position (kb) 5000 5001 5002Strain fitness (log2 ratio) -2 -1 0 1 2at 5000.138 kb on - strand, within Pf1N1B4_4532at 5000.318 kb on + strandat 5000.319 kb on - strandat 5000.378 kb on + strandat 5000.379 kb on - strandat 5000.867 kb on - strand, within Pf1N1B4_4533at 5001.107 kb on - strand, within Pf1N1B4_4533at 5001.107 kb on - strand, within Pf1N1B4_4533at 5001.562 kb on + strandat 5002.165 kb on - strand, within Pf1N1B4_4534at 5002.167 kb on + strand, within Pf1N1B4_4534at 5002.404 kb on + strand, within Pf1N1B4_4534at 5002.404 kb on + strand, within Pf1N1B4_4534at 5002.404 kb on + strand, within Pf1N1B4_4534at 5002.410 kb on + strand, within Pf1N1B4_4534at 5002.411 kb on - strand, within Pf1N1B4_4534at 5002.411 kb on - strand, within Pf1N1B4_4534

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Per-strain Table

Position Strand Gene LocusTag Fraction LB
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5,000,138 - Pf1N1B4_4532 0.87 +0.5
5,000,318 + +0.5
5,000,319 - -1.4
5,000,378 + -1.9
5,000,379 - -0.5
5,000,867 - Pf1N1B4_4533 0.38 -1.1
5,001,107 - Pf1N1B4_4533 0.62 -0.5
5,001,107 - Pf1N1B4_4533 0.62 -2.1
5,001,562 + +0.8
5,002,165 - Pf1N1B4_4534 0.39 -0.5
5,002,167 + Pf1N1B4_4534 0.39 +0.4
5,002,404 + Pf1N1B4_4534 0.52 +0.1
5,002,404 + Pf1N1B4_4534 0.52 +2.1
5,002,404 + Pf1N1B4_4534 0.52 -1.1
5,002,410 + Pf1N1B4_4534 0.53 +0.2
5,002,411 - Pf1N1B4_4534 0.53 -0.6
5,002,411 - Pf1N1B4_4534 0.53 -1.2

Or see this region's nucleotide sequence