Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4503 and Pf1N1B4_4504 are separated by 3 nucleotides Pf1N1B4_4504 and Pf1N1B4_4505 overlap by 23 nucleotides Pf1N1B4_4505 and Pf1N1B4_4506 are separated by 238 nucleotides
Pf1N1B4_4503 - Coenzyme PQQ synthesis protein B, at 4,965,547 to 4,966,461
_4503
Pf1N1B4_4504 - Pyrroloquinoline-quinone synthase (EC 1.3.3.11), at 4,966,465 to 4,966,872
_4504
Pf1N1B4_4505 - Coenzyme PQQ synthesis protein E, at 4,966,850 to 4,968,487
_4505
Pf1N1B4_4506 - Alcohol dehydrogenase (EC 1.1.1.1); Acetaldehyde dehydrogenase (EC 1.2.1.10), at 4,968,726 to 4,969,889
_4506
Position (kb)
4966
4967
4968
4969 Strain fitness (log2 ratio)
-2
-1
0
1 at 4966.359 kb on + strand, within Pf1N1B4_4503 at 4966.436 kb on - strand at 4966.449 kb on + strand at 4966.522 kb on + strand at 4966.700 kb on - strand, within Pf1N1B4_4504 at 4966.754 kb on - strand, within Pf1N1B4_4504 at 4967.118 kb on - strand, within Pf1N1B4_4505 at 4967.124 kb on - strand, within Pf1N1B4_4505 at 4967.192 kb on + strand, within Pf1N1B4_4505 at 4967.435 kb on + strand, within Pf1N1B4_4505 at 4967.732 kb on - strand, within Pf1N1B4_4505 at 4968.066 kb on - strand, within Pf1N1B4_4505 at 4968.695 kb on - strand at 4968.753 kb on - strand at 4968.810 kb on + strand at 4968.930 kb on + strand, within Pf1N1B4_4506 at 4968.930 kb on + strand, within Pf1N1B4_4506 at 4968.931 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,966,359 + Pf1N1B4_4503 0.89 -1.3 4,966,436 - -0.1 4,966,449 + +0.8 4,966,522 + -0.8 4,966,700 - Pf1N1B4_4504 0.58 -2.7 4,966,754 - Pf1N1B4_4504 0.71 +0.2 4,967,118 - Pf1N1B4_4505 0.16 +1.5 4,967,124 - Pf1N1B4_4505 0.17 +0.5 4,967,192 + Pf1N1B4_4505 0.21 +0.2 4,967,435 + Pf1N1B4_4505 0.36 +0.4 4,967,732 - Pf1N1B4_4505 0.54 -1.3 4,968,066 - Pf1N1B4_4505 0.74 +1.1 4,968,695 - -1.5 4,968,753 - -1.8 4,968,810 + +0.5 4,968,930 + Pf1N1B4_4506 0.18 -1.1 4,968,930 + Pf1N1B4_4506 0.18 +1.3 4,968,931 - -1.3
Or see this region's nucleotide sequence