Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_441 and Pf1N1B4_442 are separated by 481 nucleotides
Pf1N1B4_441 - hypothetical protein, at 523,413 to 523,679
_441
Pf1N1B4_442 - hypothetical protein, at 524,161 to 525,132
_442
Position (kb)
523
524 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 522.476 kb on - strand at 522.516 kb on + strand at 522.517 kb on - strand at 522.517 kb on - strand at 522.517 kb on - strand at 522.517 kb on - strand at 522.660 kb on + strand at 522.770 kb on - strand at 522.770 kb on - strand at 522.791 kb on + strand at 522.791 kb on + strand at 522.791 kb on + strand at 522.792 kb on - strand at 522.792 kb on - strand at 522.792 kb on - strand at 522.792 kb on - strand at 522.831 kb on - strand at 523.009 kb on + strand at 523.055 kb on + strand at 523.055 kb on + strand at 523.055 kb on + strand at 523.055 kb on + strand at 523.055 kb on + strand at 523.056 kb on - strand at 523.056 kb on - strand at 523.056 kb on - strand at 523.059 kb on + strand at 523.059 kb on + strand at 523.059 kb on + strand at 523.059 kb on + strand at 523.059 kb on + strand at 523.059 kb on + strand at 523.059 kb on + strand at 523.059 kb on + strand at 523.059 kb on + strand at 523.059 kb on + strand at 523.059 kb on + strand at 523.060 kb on - strand at 523.060 kb on - strand at 523.060 kb on - strand at 523.060 kb on - strand at 523.060 kb on - strand at 523.156 kb on + strand at 523.156 kb on + strand at 523.156 kb on + strand at 523.156 kb on + strand at 523.156 kb on + strand at 523.156 kb on + strand at 523.156 kb on + strand at 523.157 kb on - strand at 523.157 kb on - strand at 523.157 kb on - strand at 523.157 kb on - strand at 523.157 kb on - strand at 523.157 kb on - strand at 523.168 kb on + strand at 523.168 kb on + strand at 523.169 kb on - strand at 523.281 kb on + strand at 523.281 kb on + strand at 523.281 kb on + strand at 523.282 kb on - strand at 523.282 kb on - strand at 523.282 kb on - strand at 523.326 kb on + strand at 523.326 kb on + strand at 523.360 kb on + strand at 523.360 kb on + strand at 523.360 kb on + strand at 523.360 kb on + strand at 523.360 kb on + strand at 523.361 kb on - strand at 523.361 kb on - strand at 523.361 kb on - strand at 523.361 kb on - strand at 523.364 kb on + strand at 523.364 kb on + strand at 523.364 kb on + strand at 523.364 kb on + strand at 523.364 kb on + strand at 523.364 kb on + strand at 523.364 kb on + strand at 523.364 kb on + strand at 523.364 kb on + strand at 523.364 kb on + strand at 523.364 kb on + strand at 523.364 kb on + strand at 523.365 kb on - strand at 523.365 kb on - strand at 523.365 kb on - strand at 523.365 kb on - strand at 523.365 kb on - strand at 523.365 kb on - strand at 523.365 kb on - strand at 523.365 kb on - strand at 523.365 kb on - strand at 523.365 kb on - strand at 523.365 kb on - strand at 523.365 kb on - strand at 523.365 kb on - strand at 523.365 kb on - strand at 523.365 kb on - strand at 523.368 kb on + strand at 523.368 kb on + strand at 523.368 kb on + strand at 523.368 kb on + strand at 523.368 kb on + strand at 523.368 kb on + strand at 523.369 kb on - strand at 523.369 kb on - strand at 523.369 kb on - strand at 523.369 kb on - strand at 523.369 kb on - strand at 523.369 kb on - strand at 523.414 kb on + strand at 523.415 kb on - strand at 523.415 kb on - strand at 523.415 kb on - strand at 523.457 kb on + strand at 523.572 kb on + strand, within Pf1N1B4_441 at 523.572 kb on + strand, within Pf1N1B4_441 at 523.573 kb on - strand, within Pf1N1B4_441 at 523.583 kb on + strand, within Pf1N1B4_441 at 523.633 kb on + strand, within Pf1N1B4_441 at 523.634 kb on - strand, within Pf1N1B4_441 at 523.720 kb on - strand at 523.720 kb on - strand at 523.720 kb on - strand at 523.816 kb on + strand at 523.816 kb on + strand at 523.816 kb on + strand at 523.816 kb on + strand at 523.816 kb on + strand at 523.817 kb on - strand at 523.817 kb on - strand at 523.817 kb on - strand at 523.817 kb on - strand at 523.817 kb on - strand at 523.927 kb on - strand at 524.113 kb on - strand at 524.292 kb on + strand, within Pf1N1B4_442 at 524.292 kb on + strand, within Pf1N1B4_442 at 524.292 kb on + strand, within Pf1N1B4_442 at 524.293 kb on - strand, within Pf1N1B4_442 at 524.293 kb on - strand at 524.293 kb on - strand, within Pf1N1B4_442 at 524.293 kb on - strand, within Pf1N1B4_442 at 524.293 kb on - strand, within Pf1N1B4_442 at 524.293 kb on - strand, within Pf1N1B4_442 at 524.293 kb on - strand at 524.361 kb on + strand, within Pf1N1B4_442 at 524.361 kb on + strand, within Pf1N1B4_442 at 524.362 kb on - strand, within Pf1N1B4_442 at 524.362 kb on - strand, within Pf1N1B4_442 at 524.362 kb on - strand, within Pf1N1B4_442 at 524.362 kb on - strand, within Pf1N1B4_442 at 524.362 kb on - strand, within Pf1N1B4_442 at 524.412 kb on + strand, within Pf1N1B4_442 at 524.412 kb on + strand, within Pf1N1B4_442 at 524.413 kb on - strand, within Pf1N1B4_442 at 524.413 kb on - strand, within Pf1N1B4_442 at 524.515 kb on - strand, within Pf1N1B4_442 at 524.515 kb on - strand, within Pf1N1B4_442 at 524.515 kb on - strand, within Pf1N1B4_442 at 524.515 kb on - strand, within Pf1N1B4_442 at 524.573 kb on + strand, within Pf1N1B4_442 at 524.574 kb on - strand, within Pf1N1B4_442 at 524.580 kb on + strand, within Pf1N1B4_442 at 524.581 kb on - strand, within Pf1N1B4_442 at 524.624 kb on + strand, within Pf1N1B4_442 at 524.624 kb on + strand, within Pf1N1B4_442 at 524.624 kb on + strand, within Pf1N1B4_442 at 524.624 kb on + strand, within Pf1N1B4_442 at 524.624 kb on + strand at 524.624 kb on + strand, within Pf1N1B4_442 at 524.624 kb on + strand, within Pf1N1B4_442 at 524.625 kb on - strand, within Pf1N1B4_442 at 524.625 kb on - strand, within Pf1N1B4_442 at 524.625 kb on - strand, within Pf1N1B4_442 at 524.625 kb on - strand, within Pf1N1B4_442 at 524.625 kb on - strand, within Pf1N1B4_442
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 522,476 - -0.5 522,516 + -1.2 522,517 - +1.0 522,517 - +0.9 522,517 - -0.1 522,517 - -0.7 522,660 + -0.1 522,770 - +0.3 522,770 - -1.1 522,791 + -0.9 522,791 + -0.7 522,791 + -1.5 522,792 - +1.9 522,792 - -1.5 522,792 - +0.9 522,792 - -1.4 522,831 - -1.1 523,009 + +0.2 523,055 + -0.0 523,055 + +3.2 523,055 + -0.3 523,055 + -0.4 523,055 + -1.9 523,056 - +0.9 523,056 - +0.0 523,056 - -1.7 523,059 + +0.0 523,059 + +0.6 523,059 + +0.6 523,059 + +0.3 523,059 + -0.2 523,059 + -0.1 523,059 + -0.8 523,059 + +0.9 523,059 + +0.7 523,059 + -0.5 523,059 + +0.1 523,060 - +0.1 523,060 - -0.9 523,060 - -1.2 523,060 - -0.1 523,060 - -0.5 523,156 + +0.9 523,156 + -0.1 523,156 + +0.9 523,156 + +1.2 523,156 + -0.1 523,156 + +0.5 523,156 + -1.1 523,157 - -1.5 523,157 - -1.5 523,157 - -0.1 523,157 - -2.1 523,157 - -0.4 523,157 - +0.5 523,168 + -0.4 523,168 + -1.1 523,169 - +0.9 523,281 + +1.5 523,281 + -0.7 523,281 + -0.1 523,282 - +0.3 523,282 - +1.5 523,282 - -1.9 523,326 + -1.0 523,326 + +0.1 523,360 + -1.1 523,360 + -0.1 523,360 + +0.7 523,360 + +1.1 523,360 + +0.1 523,361 - -0.1 523,361 - -0.3 523,361 - -1.5 523,361 - +0.0 523,364 + -1.3 523,364 + +0.1 523,364 + -0.1 523,364 + +1.3 523,364 + +1.5 523,364 + -0.7 523,364 + -1.0 523,364 + -0.1 523,364 + -1.0 523,364 + -1.1 523,364 + +0.5 523,364 + -0.1 523,365 - +0.9 523,365 - +0.5 523,365 - -1.3 523,365 - -1.1 523,365 - +1.9 523,365 - -0.5 523,365 - -1.7 523,365 - -0.1 523,365 - -0.1 523,365 - -1.9 523,365 - -0.7 523,365 - -0.4 523,365 - -0.1 523,365 - -0.8 523,365 - -1.9 523,368 + -0.1 523,368 + -0.5 523,368 + -0.1 523,368 + -0.1 523,368 + -0.4 523,368 + -0.7 523,369 - +0.9 523,369 - +0.5 523,369 - -0.7 523,369 - +0.9 523,369 - -0.9 523,369 - -2.3 523,414 + -0.7 523,415 - -1.1 523,415 - -0.1 523,415 - +0.1 523,457 + -0.1 523,572 + Pf1N1B4_441 0.60 -0.5 523,572 + Pf1N1B4_441 0.60 -1.2 523,573 - Pf1N1B4_441 0.60 -2.1 523,583 + Pf1N1B4_441 0.64 -1.1 523,633 + Pf1N1B4_441 0.82 -0.1 523,634 - Pf1N1B4_441 0.83 +0.6 523,720 - +0.9 523,720 - -0.4 523,720 - +0.9 523,816 + +1.2 523,816 + +0.3 523,816 + -1.6 523,816 + +0.9 523,816 + -0.9 523,817 - -3.0 523,817 - +0.3 523,817 - +0.5 523,817 - -0.1 523,817 - +0.9 523,927 - -1.3 524,113 - -1.1 524,292 + Pf1N1B4_442 0.13 +0.9 524,292 + Pf1N1B4_442 0.13 -0.7 524,292 + Pf1N1B4_442 0.13 -0.9 524,293 - Pf1N1B4_442 0.14 -0.4 524,293 - -0.1 524,293 - Pf1N1B4_442 0.14 -0.7 524,293 - Pf1N1B4_442 0.14 -1.6 524,293 - Pf1N1B4_442 0.14 -0.9 524,293 - Pf1N1B4_442 0.14 +2.5 524,293 - +0.9 524,361 + Pf1N1B4_442 0.21 -0.2 524,361 + Pf1N1B4_442 0.21 +0.6 524,362 - Pf1N1B4_442 0.21 -0.4 524,362 - Pf1N1B4_442 0.21 +0.5 524,362 - Pf1N1B4_442 0.21 +0.3 524,362 - Pf1N1B4_442 0.21 +0.9 524,362 - Pf1N1B4_442 0.21 +0.5 524,412 + Pf1N1B4_442 0.26 -1.1 524,412 + Pf1N1B4_442 0.26 +0.5 524,413 - Pf1N1B4_442 0.26 -0.1 524,413 - Pf1N1B4_442 0.26 -0.7 524,515 - Pf1N1B4_442 0.36 -0.9 524,515 - Pf1N1B4_442 0.36 +0.0 524,515 - Pf1N1B4_442 0.36 -0.4 524,515 - Pf1N1B4_442 0.36 -0.1 524,573 + Pf1N1B4_442 0.42 -0.1 524,574 - Pf1N1B4_442 0.42 -0.9 524,580 + Pf1N1B4_442 0.43 -0.1 524,581 - Pf1N1B4_442 0.43 -0.7 524,624 + Pf1N1B4_442 0.48 -1.4 524,624 + Pf1N1B4_442 0.48 -0.3 524,624 + Pf1N1B4_442 0.48 -0.5 524,624 + Pf1N1B4_442 0.48 +0.1 524,624 + +0.9 524,624 + Pf1N1B4_442 0.48 +2.3 524,624 + Pf1N1B4_442 0.48 +0.3 524,625 - Pf1N1B4_442 0.48 +1.7 524,625 - Pf1N1B4_442 0.48 -1.6 524,625 - Pf1N1B4_442 0.48 -0.7 524,625 - Pf1N1B4_442 0.48 +0.2 524,625 - Pf1N1B4_442 0.48 +0.9
Or see this region's nucleotide sequence