Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4357 and Pf1N1B4_4358 are separated by 405 nucleotides Pf1N1B4_4358 and Pf1N1B4_4359 are separated by 180 nucleotides
Pf1N1B4_4357 - Putrescine utilization regulator, at 4,815,395 to 4,815,943
_4357
Pf1N1B4_4358 - Acetyltransferase, GNAT family, at 4,816,349 to 4,816,711
_4358
Pf1N1B4_4359 - PvcA protein, related to known isonitrile synthases, at 4,816,892 to 4,817,887
_4359
Position (kb)
4816
4817 Strain fitness (log2 ratio)
-2
-1
0
1 at 4815.370 kb on + strand at 4815.370 kb on + strand at 4815.371 kb on - strand at 4815.375 kb on - strand at 4815.421 kb on - strand at 4815.611 kb on + strand, within Pf1N1B4_4357 at 4815.611 kb on + strand, within Pf1N1B4_4357 at 4815.611 kb on + strand, within Pf1N1B4_4357 at 4815.611 kb on + strand, within Pf1N1B4_4357 at 4815.612 kb on - strand, within Pf1N1B4_4357 at 4815.826 kb on + strand, within Pf1N1B4_4357 at 4816.008 kb on + strand at 4816.169 kb on + strand at 4816.169 kb on + strand at 4816.170 kb on - strand at 4816.170 kb on - strand at 4816.209 kb on - strand at 4816.209 kb on - strand at 4816.334 kb on + strand at 4816.335 kb on - strand at 4816.337 kb on - strand at 4816.386 kb on - strand, within Pf1N1B4_4358 at 4816.538 kb on + strand, within Pf1N1B4_4358 at 4816.648 kb on + strand, within Pf1N1B4_4358 at 4816.648 kb on + strand, within Pf1N1B4_4358 at 4816.652 kb on + strand, within Pf1N1B4_4358 at 4816.728 kb on + strand at 4816.840 kb on + strand at 4816.892 kb on - strand at 4816.892 kb on - strand at 4817.202 kb on + strand, within Pf1N1B4_4359 at 4817.202 kb on + strand, within Pf1N1B4_4359 at 4817.203 kb on - strand, within Pf1N1B4_4359 at 4817.203 kb on - strand at 4817.203 kb on - strand at 4817.234 kb on + strand, within Pf1N1B4_4359 at 4817.234 kb on + strand, within Pf1N1B4_4359 at 4817.234 kb on + strand, within Pf1N1B4_4359 at 4817.235 kb on - strand, within Pf1N1B4_4359 at 4817.273 kb on + strand, within Pf1N1B4_4359 at 4817.274 kb on - strand at 4817.321 kb on + strand, within Pf1N1B4_4359 at 4817.510 kb on + strand, within Pf1N1B4_4359 at 4817.544 kb on - strand, within Pf1N1B4_4359 at 4817.559 kb on + strand, within Pf1N1B4_4359
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,815,370 + +0.5 4,815,370 + -0.5 4,815,371 - -0.9 4,815,375 - -1.2 4,815,421 - -0.4 4,815,611 + Pf1N1B4_4357 0.39 -0.5 4,815,611 + Pf1N1B4_4357 0.39 +0.7 4,815,611 + Pf1N1B4_4357 0.39 -1.8 4,815,611 + Pf1N1B4_4357 0.39 -1.8 4,815,612 - Pf1N1B4_4357 0.40 -0.7 4,815,826 + Pf1N1B4_4357 0.79 +0.7 4,816,008 + +1.5 4,816,169 + -0.2 4,816,169 + -2.3 4,816,170 - -0.2 4,816,170 - +0.6 4,816,209 - -0.8 4,816,209 - -0.7 4,816,334 + +0.2 4,816,335 - +0.9 4,816,337 - -1.1 4,816,386 - Pf1N1B4_4358 0.10 -1.1 4,816,538 + Pf1N1B4_4358 0.52 -2.1 4,816,648 + Pf1N1B4_4358 0.82 -0.9 4,816,648 + Pf1N1B4_4358 0.82 +0.5 4,816,652 + Pf1N1B4_4358 0.83 -0.5 4,816,728 + -0.2 4,816,840 + +1.2 4,816,892 - -0.8 4,816,892 - -0.3 4,817,202 + Pf1N1B4_4359 0.31 -0.8 4,817,202 + Pf1N1B4_4359 0.31 -1.1 4,817,203 - Pf1N1B4_4359 0.31 +0.5 4,817,203 - +1.5 4,817,203 - +0.5 4,817,234 + Pf1N1B4_4359 0.34 +0.2 4,817,234 + Pf1N1B4_4359 0.34 -0.5 4,817,234 + Pf1N1B4_4359 0.34 -2.1 4,817,235 - Pf1N1B4_4359 0.34 -0.5 4,817,273 + Pf1N1B4_4359 0.38 -0.1 4,817,274 - -0.5 4,817,321 + Pf1N1B4_4359 0.43 +1.2 4,817,510 + Pf1N1B4_4359 0.62 -0.0 4,817,544 - Pf1N1B4_4359 0.65 -2.3 4,817,559 + Pf1N1B4_4359 0.67 +0.9
Or see this region's nucleotide sequence