Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4355 and Pf1N1B4_4356 are separated by 52 nucleotides Pf1N1B4_4356 and Pf1N1B4_4357 are separated by 421 nucleotides
Pf1N1B4_4355 - Gamma-glutamyl-aminobutyraldehyde dehydrogenase (EC 1.2.1.-), at 4,812,132 to 4,813,622
_4355
Pf1N1B4_4356 - Gamma-glutamyl-putrescine oxidase (EC1.4.3.-), at 4,813,675 to 4,814,973
_4356
Pf1N1B4_4357 - Putrescine utilization regulator, at 4,815,395 to 4,815,943
_4357
Position (kb)
4813
4814
4815 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4813.194 kb on + strand, within Pf1N1B4_4355 at 4813.194 kb on + strand, within Pf1N1B4_4355 at 4813.194 kb on + strand, within Pf1N1B4_4355 at 4813.426 kb on + strand, within Pf1N1B4_4355 at 4813.500 kb on + strand at 4813.587 kb on + strand at 4813.587 kb on + strand at 4813.587 kb on + strand at 4813.587 kb on + strand at 4813.587 kb on + strand at 4813.587 kb on + strand at 4813.587 kb on + strand at 4813.588 kb on - strand at 4813.588 kb on - strand at 4813.588 kb on - strand at 4813.620 kb on + strand at 4813.629 kb on + strand at 4813.630 kb on - strand at 4813.675 kb on - strand at 4813.675 kb on - strand at 4813.706 kb on + strand at 4813.706 kb on + strand at 4813.706 kb on + strand at 4813.707 kb on - strand at 4813.715 kb on - strand at 4813.717 kb on + strand at 4814.197 kb on + strand, within Pf1N1B4_4356 at 4814.518 kb on + strand, within Pf1N1B4_4356 at 4814.518 kb on + strand, within Pf1N1B4_4356 at 4814.519 kb on - strand, within Pf1N1B4_4356 at 4814.519 kb on - strand, within Pf1N1B4_4356 at 4814.519 kb on - strand, within Pf1N1B4_4356 at 4814.539 kb on + strand, within Pf1N1B4_4356 at 4814.540 kb on - strand, within Pf1N1B4_4356 at 4814.764 kb on + strand, within Pf1N1B4_4356 at 4814.767 kb on + strand at 4814.767 kb on + strand, within Pf1N1B4_4356 at 4814.767 kb on + strand, within Pf1N1B4_4356 at 4814.767 kb on + strand at 4814.767 kb on + strand, within Pf1N1B4_4356 at 4814.767 kb on + strand, within Pf1N1B4_4356 at 4814.767 kb on + strand, within Pf1N1B4_4356 at 4814.768 kb on - strand, within Pf1N1B4_4356 at 4814.768 kb on - strand, within Pf1N1B4_4356 at 4814.768 kb on - strand, within Pf1N1B4_4356 at 4814.942 kb on - strand at 4814.967 kb on + strand at 4814.971 kb on + strand at 4814.971 kb on + strand at 4814.971 kb on + strand at 4814.971 kb on + strand at 4814.972 kb on - strand at 4814.972 kb on - strand at 4815.017 kb on - strand at 4815.231 kb on - strand at 4815.231 kb on - strand at 4815.287 kb on - strand at 4815.287 kb on - strand at 4815.370 kb on + strand at 4815.370 kb on + strand at 4815.371 kb on - strand at 4815.375 kb on - strand at 4815.421 kb on - strand at 4815.611 kb on + strand, within Pf1N1B4_4357 at 4815.611 kb on + strand, within Pf1N1B4_4357 at 4815.611 kb on + strand, within Pf1N1B4_4357 at 4815.611 kb on + strand, within Pf1N1B4_4357 at 4815.612 kb on - strand, within Pf1N1B4_4357 at 4815.826 kb on + strand, within Pf1N1B4_4357
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,813,194 + Pf1N1B4_4355 0.71 +0.3 4,813,194 + Pf1N1B4_4355 0.71 -0.5 4,813,194 + Pf1N1B4_4355 0.71 +0.1 4,813,426 + Pf1N1B4_4355 0.87 -2.7 4,813,500 + +0.1 4,813,587 + +1.1 4,813,587 + -2.8 4,813,587 + -0.5 4,813,587 + -0.2 4,813,587 + -0.8 4,813,587 + -0.6 4,813,587 + -0.1 4,813,588 - -0.8 4,813,588 - +0.5 4,813,588 - +0.9 4,813,620 + +0.1 4,813,629 + +0.3 4,813,630 - -0.5 4,813,675 - -1.5 4,813,675 - +0.2 4,813,706 + +0.5 4,813,706 + -0.8 4,813,706 + -0.5 4,813,707 - +0.4 4,813,715 - -0.8 4,813,717 + -0.8 4,814,197 + Pf1N1B4_4356 0.40 -0.7 4,814,518 + Pf1N1B4_4356 0.65 -0.2 4,814,518 + Pf1N1B4_4356 0.65 -0.0 4,814,519 - Pf1N1B4_4356 0.65 -0.1 4,814,519 - Pf1N1B4_4356 0.65 +0.3 4,814,519 - Pf1N1B4_4356 0.65 +1.7 4,814,539 + Pf1N1B4_4356 0.67 +0.1 4,814,540 - Pf1N1B4_4356 0.67 +1.7 4,814,764 + Pf1N1B4_4356 0.84 -0.3 4,814,767 + +1.8 4,814,767 + Pf1N1B4_4356 0.84 +0.3 4,814,767 + Pf1N1B4_4356 0.84 -0.2 4,814,767 + -0.5 4,814,767 + Pf1N1B4_4356 0.84 +1.0 4,814,767 + Pf1N1B4_4356 0.84 -0.2 4,814,767 + Pf1N1B4_4356 0.84 -1.7 4,814,768 - Pf1N1B4_4356 0.84 +1.2 4,814,768 - Pf1N1B4_4356 0.84 -0.5 4,814,768 - Pf1N1B4_4356 0.84 -0.8 4,814,942 - -0.5 4,814,967 + +0.5 4,814,971 + -0.5 4,814,971 + +0.5 4,814,971 + -1.8 4,814,971 + -0.7 4,814,972 - -1.8 4,814,972 - -1.1 4,815,017 - +0.5 4,815,231 - -0.1 4,815,231 - +0.9 4,815,287 - -1.5 4,815,287 - +0.1 4,815,370 + +0.5 4,815,370 + -0.5 4,815,371 - -0.9 4,815,375 - -1.2 4,815,421 - -0.4 4,815,611 + Pf1N1B4_4357 0.39 -0.5 4,815,611 + Pf1N1B4_4357 0.39 +0.7 4,815,611 + Pf1N1B4_4357 0.39 -1.8 4,815,611 + Pf1N1B4_4357 0.39 -1.8 4,815,612 - Pf1N1B4_4357 0.40 -0.7 4,815,826 + Pf1N1B4_4357 0.79 +0.7
Or see this region's nucleotide sequence