Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4271 and Pf1N1B4_4272 are separated by 173 nucleotides Pf1N1B4_4272 and Pf1N1B4_4273 are separated by 74 nucleotides Pf1N1B4_4273 and Pf1N1B4_4274 are separated by 311 nucleotides
Pf1N1B4_4271 - Hydrogen cyanide synthase HcnC / Opine oxidase subunit B, at 4,735,047 to 4,736,306
_4271
Pf1N1B4_4272 - Glutathione S-transferase, unnamed subgroup 2 (EC 2.5.1.18), at 4,736,480 to 4,737,091
_4272
Pf1N1B4_4273 - Predicted transcriptional regulator of the myo-inositol catabolic operon, at 4,737,166 to 4,738,086
_4273
Pf1N1B4_4274 - 5-keto-2-deoxygluconokinase (EC 2.7.1.92) / uncharacterized domain, at 4,738,398 to 4,740,335
_4274
Position (kb)
4737
4738
4739 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4736.256 kb on - strand at 4736.335 kb on + strand at 4736.353 kb on + strand at 4736.524 kb on - strand at 4736.524 kb on - strand at 4736.615 kb on - strand at 4737.060 kb on + strand at 4737.131 kb on + strand at 4737.132 kb on - strand at 4737.220 kb on - strand at 4737.220 kb on - strand at 4737.221 kb on + strand at 4737.222 kb on - strand at 4737.539 kb on + strand, within Pf1N1B4_4273 at 4737.539 kb on + strand, within Pf1N1B4_4273 at 4737.587 kb on + strand, within Pf1N1B4_4273 at 4737.587 kb on + strand at 4737.587 kb on + strand, within Pf1N1B4_4273 at 4737.587 kb on + strand at 4737.588 kb on - strand, within Pf1N1B4_4273 at 4737.761 kb on + strand, within Pf1N1B4_4273 at 4737.875 kb on - strand, within Pf1N1B4_4273 at 4737.875 kb on - strand, within Pf1N1B4_4273 at 4737.941 kb on + strand, within Pf1N1B4_4273 at 4737.941 kb on + strand at 4737.942 kb on - strand, within Pf1N1B4_4273 at 4737.942 kb on - strand, within Pf1N1B4_4273 at 4737.942 kb on - strand, within Pf1N1B4_4273 at 4737.980 kb on + strand, within Pf1N1B4_4273 at 4737.981 kb on - strand, within Pf1N1B4_4273 at 4738.087 kb on - strand at 4738.144 kb on + strand at 4738.144 kb on + strand at 4738.145 kb on - strand at 4738.145 kb on - strand at 4738.456 kb on + strand at 4738.470 kb on + strand at 4738.521 kb on + strand at 4738.884 kb on + strand, within Pf1N1B4_4274 at 4738.884 kb on + strand, within Pf1N1B4_4274 at 4738.884 kb on + strand, within Pf1N1B4_4274
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,736,256 - +0.2 4,736,335 + -0.3 4,736,353 + +0.5 4,736,524 - -1.7 4,736,524 - +0.9 4,736,615 - +2.1 4,737,060 + -0.9 4,737,131 + +0.8 4,737,132 - +0.5 4,737,220 - -1.3 4,737,220 - -0.1 4,737,221 + +0.3 4,737,222 - +0.5 4,737,539 + Pf1N1B4_4273 0.40 -0.7 4,737,539 + Pf1N1B4_4273 0.40 -0.5 4,737,587 + Pf1N1B4_4273 0.46 +0.1 4,737,587 + +1.1 4,737,587 + Pf1N1B4_4273 0.46 +0.5 4,737,587 + -0.5 4,737,588 - Pf1N1B4_4273 0.46 -2.3 4,737,761 + Pf1N1B4_4273 0.65 -2.1 4,737,875 - Pf1N1B4_4273 0.77 -0.7 4,737,875 - Pf1N1B4_4273 0.77 -1.8 4,737,941 + Pf1N1B4_4273 0.84 -1.0 4,737,941 + -0.1 4,737,942 - Pf1N1B4_4273 0.84 +0.5 4,737,942 - Pf1N1B4_4273 0.84 -2.1 4,737,942 - Pf1N1B4_4273 0.84 -0.8 4,737,980 + Pf1N1B4_4273 0.88 -0.7 4,737,981 - Pf1N1B4_4273 0.88 +0.2 4,738,087 - -0.6 4,738,144 + -3.4 4,738,144 + -1.1 4,738,145 - +0.5 4,738,145 - +2.1 4,738,456 + -0.5 4,738,470 + -1.1 4,738,521 + -1.5 4,738,884 + Pf1N1B4_4274 0.25 -0.1 4,738,884 + Pf1N1B4_4274 0.25 -0.3 4,738,884 + Pf1N1B4_4274 0.25 -0.5
Or see this region's nucleotide sequence