Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4172 and Pf1N1B4_4173 are separated by 55 nucleotides Pf1N1B4_4173 and Pf1N1B4_4174 are separated by 55 nucleotides Pf1N1B4_4174 and Pf1N1B4_4175 overlap by 15 nucleotides Pf1N1B4_4175 and Pf1N1B4_4176 overlap by 11 nucleotides
Pf1N1B4_4172 - hypothetical protein, at 4,622,770 to 4,622,904
_4172
Pf1N1B4_4173 - Acetyl-CoA synthetase (ADP-forming) alpha and beta chains, putative, at 4,622,960 to 4,623,523
_4173
Pf1N1B4_4174 - hypothetical protein, at 4,623,579 to 4,623,761
_4174
Pf1N1B4_4175 - Universal stress protein family 5, at 4,623,747 to 4,624,667
_4175
Pf1N1B4_4176 - Alcohol dehydrogenase (EC 1.1.1.1), at 4,624,657 to 4,625,640
_4176
Position (kb)
4623
4624
4625 Strain fitness (log2 ratio)
-2
-1
0
1 at 4622.837 kb on + strand, within Pf1N1B4_4172 at 4622.837 kb on + strand, within Pf1N1B4_4172 at 4622.838 kb on - strand, within Pf1N1B4_4172 at 4622.838 kb on - strand, within Pf1N1B4_4172 at 4622.843 kb on + strand, within Pf1N1B4_4172 at 4622.967 kb on + strand at 4622.967 kb on + strand at 4623.075 kb on + strand, within Pf1N1B4_4173 at 4623.075 kb on + strand, within Pf1N1B4_4173 at 4623.076 kb on - strand, within Pf1N1B4_4173 at 4623.076 kb on - strand, within Pf1N1B4_4173 at 4623.261 kb on + strand, within Pf1N1B4_4173 at 4623.262 kb on - strand, within Pf1N1B4_4173 at 4623.262 kb on - strand, within Pf1N1B4_4173 at 4623.262 kb on - strand, within Pf1N1B4_4173 at 4623.262 kb on - strand, within Pf1N1B4_4173 at 4623.262 kb on - strand, within Pf1N1B4_4173 at 4623.262 kb on - strand, within Pf1N1B4_4173 at 4623.262 kb on - strand, within Pf1N1B4_4173 at 4623.385 kb on - strand, within Pf1N1B4_4173 at 4623.667 kb on - strand, within Pf1N1B4_4174 at 4623.709 kb on + strand, within Pf1N1B4_4174 at 4623.709 kb on + strand, within Pf1N1B4_4174 at 4623.792 kb on + strand at 4623.868 kb on + strand, within Pf1N1B4_4175 at 4623.868 kb on + strand, within Pf1N1B4_4175 at 4623.868 kb on + strand, within Pf1N1B4_4175 at 4623.868 kb on + strand, within Pf1N1B4_4175 at 4623.869 kb on - strand, within Pf1N1B4_4175 at 4623.869 kb on - strand at 4624.306 kb on + strand at 4624.329 kb on + strand, within Pf1N1B4_4175 at 4624.329 kb on + strand at 4624.329 kb on + strand, within Pf1N1B4_4175 at 4624.329 kb on + strand, within Pf1N1B4_4175 at 4624.330 kb on - strand, within Pf1N1B4_4175 at 4624.525 kb on - strand, within Pf1N1B4_4175 at 4624.525 kb on - strand, within Pf1N1B4_4175 at 4624.543 kb on + strand at 4624.544 kb on - strand, within Pf1N1B4_4175 at 4624.764 kb on + strand, within Pf1N1B4_4176 at 4624.764 kb on + strand, within Pf1N1B4_4176 at 4625.036 kb on + strand at 4625.036 kb on + strand, within Pf1N1B4_4176 at 4625.036 kb on + strand, within Pf1N1B4_4176 at 4625.076 kb on - strand, within Pf1N1B4_4176 at 4625.076 kb on - strand, within Pf1N1B4_4176 at 4625.276 kb on + strand, within Pf1N1B4_4176 at 4625.555 kb on - strand at 4625.643 kb on + strand at 4625.644 kb on - strand at 4625.644 kb on - strand at 4625.644 kb on - strand at 4625.644 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,622,837 + Pf1N1B4_4172 0.50 -0.2 4,622,837 + Pf1N1B4_4172 0.50 -1.1 4,622,838 - Pf1N1B4_4172 0.50 +1.2 4,622,838 - Pf1N1B4_4172 0.50 -0.1 4,622,843 + Pf1N1B4_4172 0.54 -0.4 4,622,967 + -0.4 4,622,967 + -1.7 4,623,075 + Pf1N1B4_4173 0.20 +1.3 4,623,075 + Pf1N1B4_4173 0.20 -0.2 4,623,076 - Pf1N1B4_4173 0.21 -2.7 4,623,076 - Pf1N1B4_4173 0.21 +0.0 4,623,261 + Pf1N1B4_4173 0.53 -1.0 4,623,262 - Pf1N1B4_4173 0.54 -0.6 4,623,262 - Pf1N1B4_4173 0.54 -1.1 4,623,262 - Pf1N1B4_4173 0.54 +0.0 4,623,262 - Pf1N1B4_4173 0.54 +1.4 4,623,262 - Pf1N1B4_4173 0.54 -0.4 4,623,262 - Pf1N1B4_4173 0.54 -0.4 4,623,262 - Pf1N1B4_4173 0.54 +0.4 4,623,385 - Pf1N1B4_4173 0.75 -0.0 4,623,667 - Pf1N1B4_4174 0.48 -1.1 4,623,709 + Pf1N1B4_4174 0.71 -0.4 4,623,709 + Pf1N1B4_4174 0.71 -0.5 4,623,792 + -0.8 4,623,868 + Pf1N1B4_4175 0.13 -0.2 4,623,868 + Pf1N1B4_4175 0.13 +0.6 4,623,868 + Pf1N1B4_4175 0.13 -0.6 4,623,868 + Pf1N1B4_4175 0.13 +0.0 4,623,869 - Pf1N1B4_4175 0.13 +0.9 4,623,869 - +0.0 4,624,306 + +1.6 4,624,329 + Pf1N1B4_4175 0.63 +1.6 4,624,329 + -1.4 4,624,329 + Pf1N1B4_4175 0.63 +0.6 4,624,329 + Pf1N1B4_4175 0.63 +1.4 4,624,330 - Pf1N1B4_4175 0.63 +0.9 4,624,525 - Pf1N1B4_4175 0.84 +0.6 4,624,525 - Pf1N1B4_4175 0.84 -0.4 4,624,543 + -0.4 4,624,544 - Pf1N1B4_4175 0.87 -0.2 4,624,764 + Pf1N1B4_4176 0.11 -0.1 4,624,764 + Pf1N1B4_4176 0.11 +0.0 4,625,036 + -0.4 4,625,036 + Pf1N1B4_4176 0.39 +0.2 4,625,036 + Pf1N1B4_4176 0.39 +1.7 4,625,076 - Pf1N1B4_4176 0.43 -0.2 4,625,076 - Pf1N1B4_4176 0.43 +0.2 4,625,276 + Pf1N1B4_4176 0.63 -0.6 4,625,555 - -1.2 4,625,643 + -1.3 4,625,644 - -1.0 4,625,644 - +0.0 4,625,644 - +0.6 4,625,644 - -1.0
Or see this region's nucleotide sequence