Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4081 and Pf1N1B4_4082 overlap by 7 nucleotides Pf1N1B4_4082 and Pf1N1B4_4083 are separated by 133 nucleotides Pf1N1B4_4083 and Pf1N1B4_4084 are separated by 96 nucleotides Pf1N1B4_4084 and Pf1N1B4_4085 are separated by 168 nucleotides
Pf1N1B4_4081 - Gamma-glutamyltranspeptidase (EC 2.3.2.2), at 4,530,360 to 4,532,087
_4081
Pf1N1B4_4082 - hypothetical protein, at 4,532,081 to 4,532,221
_4082
Pf1N1B4_4083 - Isochorismatase (EC 3.3.2.1), at 4,532,355 to 4,532,888
_4083
Pf1N1B4_4084 - hypothetical protein, at 4,532,985 to 4,533,275
_4084
Pf1N1B4_4085 - probable exported protein YPO0702, at 4,533,444 to 4,534,475
_4085
Position (kb)
4532
4533 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4531.484 kb on + strand, within Pf1N1B4_4081 at 4531.484 kb on + strand, within Pf1N1B4_4081 at 4531.485 kb on - strand, within Pf1N1B4_4081 at 4531.536 kb on + strand, within Pf1N1B4_4081 at 4531.536 kb on + strand, within Pf1N1B4_4081 at 4531.537 kb on - strand, within Pf1N1B4_4081 at 4531.537 kb on - strand, within Pf1N1B4_4081 at 4531.537 kb on - strand, within Pf1N1B4_4081 at 4531.537 kb on - strand, within Pf1N1B4_4081 at 4531.644 kb on + strand, within Pf1N1B4_4081 at 4531.644 kb on + strand, within Pf1N1B4_4081 at 4531.644 kb on + strand, within Pf1N1B4_4081 at 4531.645 kb on - strand, within Pf1N1B4_4081 at 4531.645 kb on - strand, within Pf1N1B4_4081 at 4531.645 kb on - strand, within Pf1N1B4_4081 at 4531.645 kb on - strand, within Pf1N1B4_4081 at 4531.645 kb on - strand at 4531.645 kb on - strand, within Pf1N1B4_4081 at 4531.645 kb on - strand, within Pf1N1B4_4081 at 4531.982 kb on + strand at 4531.982 kb on + strand at 4531.983 kb on - strand at 4531.991 kb on - strand at 4532.060 kb on + strand at 4532.060 kb on + strand at 4532.060 kb on + strand at 4532.061 kb on - strand at 4532.138 kb on - strand, within Pf1N1B4_4082 at 4532.212 kb on - strand at 4532.279 kb on - strand at 4532.296 kb on - strand at 4532.339 kb on - strand at 4532.529 kb on + strand, within Pf1N1B4_4083 at 4532.529 kb on + strand, within Pf1N1B4_4083 at 4532.530 kb on - strand, within Pf1N1B4_4083 at 4532.616 kb on + strand, within Pf1N1B4_4083 at 4532.616 kb on + strand, within Pf1N1B4_4083 at 4532.616 kb on + strand, within Pf1N1B4_4083 at 4532.691 kb on + strand, within Pf1N1B4_4083 at 4532.692 kb on - strand, within Pf1N1B4_4083 at 4532.947 kb on + strand at 4532.948 kb on - strand at 4533.080 kb on - strand, within Pf1N1B4_4084 at 4533.239 kb on - strand, within Pf1N1B4_4084 at 4533.381 kb on - strand at 4533.413 kb on + strand at 4533.413 kb on + strand at 4533.414 kb on - strand at 4533.414 kb on - strand at 4533.467 kb on + strand at 4533.468 kb on - strand at 4533.527 kb on + strand at 4533.661 kb on + strand, within Pf1N1B4_4085 at 4533.662 kb on - strand, within Pf1N1B4_4085 at 4533.662 kb on - strand, within Pf1N1B4_4085 at 4533.771 kb on - strand, within Pf1N1B4_4085 at 4533.866 kb on - strand, within Pf1N1B4_4085
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,531,484 + Pf1N1B4_4081 0.65 +1.0 4,531,484 + Pf1N1B4_4081 0.65 -0.4 4,531,485 - Pf1N1B4_4081 0.65 +0.2 4,531,536 + Pf1N1B4_4081 0.68 -1.4 4,531,536 + Pf1N1B4_4081 0.68 +0.8 4,531,537 - Pf1N1B4_4081 0.68 -1.2 4,531,537 - Pf1N1B4_4081 0.68 -0.4 4,531,537 - Pf1N1B4_4081 0.68 +3.6 4,531,537 - Pf1N1B4_4081 0.68 -0.6 4,531,644 + Pf1N1B4_4081 0.74 -0.6 4,531,644 + Pf1N1B4_4081 0.74 -0.4 4,531,644 + Pf1N1B4_4081 0.74 -1.2 4,531,645 - Pf1N1B4_4081 0.74 +0.2 4,531,645 - Pf1N1B4_4081 0.74 -0.6 4,531,645 - Pf1N1B4_4081 0.74 +0.2 4,531,645 - Pf1N1B4_4081 0.74 +1.6 4,531,645 - +0.1 4,531,645 - Pf1N1B4_4081 0.74 -0.0 4,531,645 - Pf1N1B4_4081 0.74 -0.5 4,531,982 + +0.1 4,531,982 + +0.3 4,531,983 - -0.8 4,531,991 - -0.4 4,532,060 + -1.5 4,532,060 + -0.9 4,532,060 + -0.7 4,532,061 - -0.7 4,532,138 - Pf1N1B4_4082 0.40 -0.1 4,532,212 - -0.9 4,532,279 - +1.6 4,532,296 - -0.2 4,532,339 - -0.3 4,532,529 + Pf1N1B4_4083 0.33 +0.2 4,532,529 + Pf1N1B4_4083 0.33 -1.4 4,532,530 - Pf1N1B4_4083 0.33 -0.0 4,532,616 + Pf1N1B4_4083 0.49 -0.4 4,532,616 + Pf1N1B4_4083 0.49 -0.8 4,532,616 + Pf1N1B4_4083 0.49 -1.4 4,532,691 + Pf1N1B4_4083 0.63 -0.6 4,532,692 - Pf1N1B4_4083 0.63 -1.7 4,532,947 + +0.4 4,532,948 - +1.2 4,533,080 - Pf1N1B4_4084 0.33 -0.6 4,533,239 - Pf1N1B4_4084 0.87 +0.6 4,533,381 - -0.4 4,533,413 + -0.9 4,533,413 + -2.2 4,533,414 - +1.9 4,533,414 - +1.1 4,533,467 + -0.2 4,533,468 - -0.4 4,533,527 + -2.5 4,533,661 + Pf1N1B4_4085 0.21 -0.0 4,533,662 - Pf1N1B4_4085 0.21 -1.0 4,533,662 - Pf1N1B4_4085 0.21 +0.5 4,533,771 - Pf1N1B4_4085 0.32 +0.9 4,533,866 - Pf1N1B4_4085 0.41 +1.6
Or see this region's nucleotide sequence