Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4029 and Pf1N1B4_4030 are separated by 39 nucleotides Pf1N1B4_4030 and Pf1N1B4_4031 overlap by 4 nucleotides Pf1N1B4_4031 and Pf1N1B4_4032 are separated by 2 nucleotides Pf1N1B4_4032 and Pf1N1B4_4033 are separated by 9 nucleotides
Pf1N1B4_4029 - NADH-ubiquinone oxidoreductase chain M (EC 1.6.5.3), at 4,479,723 to 4,481,255
_4029
Pf1N1B4_4030 - NADH-ubiquinone oxidoreductase chain L (EC 1.6.5.3), at 4,481,295 to 4,483,148
_4030
Pf1N1B4_4031 - NADH-ubiquinone oxidoreductase chain K (EC 1.6.5.3), at 4,483,145 to 4,483,453
_4031
Pf1N1B4_4032 - NADH-ubiquinone oxidoreductase chain J (EC 1.6.5.3), at 4,483,456 to 4,483,959
_4032
Pf1N1B4_4033 - NADH-ubiquinone oxidoreductase chain I (EC 1.6.5.3), at 4,483,969 to 4,484,514
_4033
Position (kb)
4481
4482
4483
4484 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4480.352 kb on - strand, within Pf1N1B4_4029 at 4480.444 kb on + strand, within Pf1N1B4_4029 at 4480.444 kb on + strand, within Pf1N1B4_4029 at 4480.588 kb on + strand, within Pf1N1B4_4029 at 4480.801 kb on + strand, within Pf1N1B4_4029 at 4480.802 kb on - strand, within Pf1N1B4_4029 at 4481.325 kb on + strand at 4481.861 kb on - strand, within Pf1N1B4_4030 at 4481.861 kb on - strand, within Pf1N1B4_4030 at 4481.954 kb on - strand, within Pf1N1B4_4030 at 4482.168 kb on + strand, within Pf1N1B4_4030 at 4482.215 kb on - strand, within Pf1N1B4_4030 at 4482.826 kb on + strand, within Pf1N1B4_4030 at 4482.923 kb on + strand, within Pf1N1B4_4030 at 4483.005 kb on + strand at 4483.006 kb on - strand at 4483.198 kb on - strand, within Pf1N1B4_4031 at 4483.634 kb on + strand, within Pf1N1B4_4032 at 4483.635 kb on - strand, within Pf1N1B4_4032 at 4484.060 kb on + strand, within Pf1N1B4_4033 at 4484.061 kb on - strand at 4484.069 kb on + strand, within Pf1N1B4_4033 at 4484.070 kb on - strand, within Pf1N1B4_4033 at 4484.070 kb on - strand, within Pf1N1B4_4033
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,480,352 - Pf1N1B4_4029 0.41 -0.6 4,480,444 + Pf1N1B4_4029 0.47 -0.6 4,480,444 + Pf1N1B4_4029 0.47 -0.3 4,480,588 + Pf1N1B4_4029 0.56 -0.5 4,480,801 + Pf1N1B4_4029 0.70 -0.9 4,480,802 - Pf1N1B4_4029 0.70 -1.1 4,481,325 + -1.8 4,481,861 - Pf1N1B4_4030 0.31 -0.9 4,481,861 - Pf1N1B4_4030 0.31 -0.7 4,481,954 - Pf1N1B4_4030 0.36 -0.3 4,482,168 + Pf1N1B4_4030 0.47 -0.5 4,482,215 - Pf1N1B4_4030 0.50 +1.3 4,482,826 + Pf1N1B4_4030 0.83 -0.9 4,482,923 + Pf1N1B4_4030 0.88 -1.0 4,483,005 + +0.6 4,483,006 - +1.4 4,483,198 - Pf1N1B4_4031 0.17 -3.0 4,483,634 + Pf1N1B4_4032 0.35 -0.9 4,483,635 - Pf1N1B4_4032 0.36 -0.3 4,484,060 + Pf1N1B4_4033 0.17 -2.6 4,484,061 - +0.7 4,484,069 + Pf1N1B4_4033 0.18 -1.6 4,484,070 - Pf1N1B4_4033 0.18 +1.3 4,484,070 - Pf1N1B4_4033 0.18 -1.2
Or see this region's nucleotide sequence