Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3807 and Pf1N1B4_3808 are separated by 263 nucleotides Pf1N1B4_3808 and Pf1N1B4_3809 are separated by 111 nucleotides Pf1N1B4_3809 and Pf1N1B4_3810 are separated by 124 nucleotides
Pf1N1B4_3807 - Rhs family protein, at 4,214,344 to 4,219,221
_3807
Pf1N1B4_3808 - hydrolase, haloacid dehalogenase-like family, at 4,219,485 to 4,220,180
_3808
Pf1N1B4_3809 - Oxidoreductase, short chain dehydrogenase/reductase family, at 4,220,292 to 4,221,038
_3809
Pf1N1B4_3810 - Transcriptional regulator, LysR family, at 4,221,163 to 4,222,089
_3810
Position (kb)
4219
4220
4221 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4218.708 kb on - strand, within Pf1N1B4_3807 at 4218.716 kb on + strand, within Pf1N1B4_3807 at 4218.716 kb on + strand, within Pf1N1B4_3807 at 4218.716 kb on + strand at 4218.716 kb on + strand, within Pf1N1B4_3807 at 4218.717 kb on - strand, within Pf1N1B4_3807 at 4218.717 kb on - strand, within Pf1N1B4_3807 at 4218.717 kb on - strand, within Pf1N1B4_3807 at 4218.717 kb on - strand, within Pf1N1B4_3807 at 4218.717 kb on - strand at 4218.859 kb on - strand at 4218.930 kb on + strand at 4218.930 kb on + strand at 4218.930 kb on + strand at 4218.931 kb on - strand at 4219.402 kb on - strand at 4219.418 kb on + strand at 4219.528 kb on + strand at 4219.565 kb on + strand, within Pf1N1B4_3808 at 4219.569 kb on + strand, within Pf1N1B4_3808 at 4219.569 kb on + strand, within Pf1N1B4_3808 at 4219.569 kb on + strand, within Pf1N1B4_3808 at 4219.569 kb on + strand, within Pf1N1B4_3808 at 4219.569 kb on + strand, within Pf1N1B4_3808 at 4219.569 kb on + strand, within Pf1N1B4_3808 at 4219.570 kb on - strand, within Pf1N1B4_3808 at 4219.671 kb on + strand, within Pf1N1B4_3808 at 4219.671 kb on + strand, within Pf1N1B4_3808 at 4219.700 kb on + strand, within Pf1N1B4_3808 at 4219.700 kb on + strand, within Pf1N1B4_3808 at 4219.817 kb on + strand, within Pf1N1B4_3808 at 4220.123 kb on + strand at 4220.124 kb on - strand at 4220.185 kb on + strand at 4220.185 kb on + strand at 4220.186 kb on - strand at 4220.186 kb on - strand at 4220.186 kb on - strand at 4220.249 kb on + strand at 4220.250 kb on - strand at 4220.339 kb on - strand at 4220.339 kb on - strand at 4220.362 kb on + strand at 4220.363 kb on - strand at 4220.363 kb on - strand at 4220.363 kb on - strand at 4220.363 kb on - strand at 4220.363 kb on - strand at 4220.363 kb on - strand at 4220.569 kb on + strand at 4220.923 kb on + strand, within Pf1N1B4_3809 at 4220.923 kb on + strand, within Pf1N1B4_3809 at 4220.923 kb on + strand, within Pf1N1B4_3809 at 4220.923 kb on + strand, within Pf1N1B4_3809 at 4220.924 kb on - strand, within Pf1N1B4_3809 at 4220.924 kb on - strand, within Pf1N1B4_3809 at 4220.924 kb on - strand, within Pf1N1B4_3809 at 4221.009 kb on + strand at 4221.009 kb on + strand at 4221.009 kb on + strand at 4221.010 kb on - strand at 4221.010 kb on - strand at 4221.010 kb on - strand at 4221.101 kb on + strand at 4221.101 kb on + strand at 4221.101 kb on + strand at 4221.112 kb on + strand at 4221.126 kb on + strand at 4221.126 kb on + strand at 4221.127 kb on - strand at 4221.129 kb on - strand at 4221.129 kb on - strand at 4221.137 kb on + strand at 4221.150 kb on + strand at 4221.150 kb on + strand at 4221.150 kb on + strand at 4221.152 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,218,708 - Pf1N1B4_3807 0.89 +2.2 4,218,716 + Pf1N1B4_3807 0.90 -0.0 4,218,716 + Pf1N1B4_3807 0.90 +0.1 4,218,716 + -0.2 4,218,716 + Pf1N1B4_3807 0.90 -0.2 4,218,717 - Pf1N1B4_3807 0.90 +0.0 4,218,717 - Pf1N1B4_3807 0.90 -0.7 4,218,717 - Pf1N1B4_3807 0.90 +0.5 4,218,717 - Pf1N1B4_3807 0.90 -2.4 4,218,717 - +0.0 4,218,859 - +0.2 4,218,930 + +1.5 4,218,930 + -1.6 4,218,930 + +1.8 4,218,931 - +0.8 4,219,402 - -2.4 4,219,418 + +0.4 4,219,528 + -0.1 4,219,565 + Pf1N1B4_3808 0.11 +0.8 4,219,569 + Pf1N1B4_3808 0.12 -2.3 4,219,569 + Pf1N1B4_3808 0.12 -0.1 4,219,569 + Pf1N1B4_3808 0.12 +2.1 4,219,569 + Pf1N1B4_3808 0.12 +0.8 4,219,569 + Pf1N1B4_3808 0.12 +0.9 4,219,569 + Pf1N1B4_3808 0.12 +1.8 4,219,570 - Pf1N1B4_3808 0.12 +0.0 4,219,671 + Pf1N1B4_3808 0.27 -1.6 4,219,671 + Pf1N1B4_3808 0.27 -1.4 4,219,700 + Pf1N1B4_3808 0.31 +0.8 4,219,700 + Pf1N1B4_3808 0.31 +0.3 4,219,817 + Pf1N1B4_3808 0.48 +1.0 4,220,123 + +0.8 4,220,124 - -1.6 4,220,185 + +1.5 4,220,185 + -0.7 4,220,186 - -2.4 4,220,186 - -0.2 4,220,186 - +0.5 4,220,249 + +0.2 4,220,250 - -2.0 4,220,339 - +0.0 4,220,339 - -1.2 4,220,362 + +2.6 4,220,363 - -1.4 4,220,363 - +0.8 4,220,363 - +0.4 4,220,363 - -1.0 4,220,363 - -0.2 4,220,363 - +1.1 4,220,569 + +1.8 4,220,923 + Pf1N1B4_3809 0.84 -1.2 4,220,923 + Pf1N1B4_3809 0.84 -2.2 4,220,923 + Pf1N1B4_3809 0.84 -0.2 4,220,923 + Pf1N1B4_3809 0.84 -1.1 4,220,924 - Pf1N1B4_3809 0.85 -0.2 4,220,924 - Pf1N1B4_3809 0.85 -0.1 4,220,924 - Pf1N1B4_3809 0.85 -0.3 4,221,009 + -0.7 4,221,009 + +1.0 4,221,009 + -0.8 4,221,010 - -0.7 4,221,010 - +0.0 4,221,010 - +0.0 4,221,101 + +0.3 4,221,101 + -0.8 4,221,101 + -0.8 4,221,112 + +0.3 4,221,126 + -0.8 4,221,126 + +0.3 4,221,127 - +0.0 4,221,129 - -0.4 4,221,129 - +0.2 4,221,137 + +0.6 4,221,150 + -0.3 4,221,150 + -0.4 4,221,150 + +1.5 4,221,152 + +0.8
Or see this region's nucleotide sequence