Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_37 and Pf1N1B4_38 are separated by 113 nucleotides Pf1N1B4_38 and Pf1N1B4_39 are separated by 100 nucleotides
Pf1N1B4_37 - N-hydroxyarylamine O-acetyltransferase (EC 2.3.1.118), at 42,059 to 42,898
_37
Pf1N1B4_38 - Exodeoxyribonuclease III (EC 3.1.11.2), at 43,012 to 43,806
_38
Pf1N1B4_39 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 43,907 to 46,477
_39
Position (kb) 43
44 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 42.233 kb on - strand, within Pf1N1B4_37 at 42.275 kb on + strand, within Pf1N1B4_37 at 42.315 kb on + strand, within Pf1N1B4_37 at 42.316 kb on - strand, within Pf1N1B4_37 at 42.316 kb on - strand, within Pf1N1B4_37 at 42.369 kb on + strand, within Pf1N1B4_37 at 42.369 kb on + strand, within Pf1N1B4_37 at 42.369 kb on + strand, within Pf1N1B4_37 at 42.369 kb on + strand, within Pf1N1B4_37 at 42.370 kb on - strand, within Pf1N1B4_37 at 42.370 kb on - strand, within Pf1N1B4_37 at 42.370 kb on - strand, within Pf1N1B4_37 at 42.370 kb on - strand, within Pf1N1B4_37 at 42.411 kb on + strand, within Pf1N1B4_37 at 42.528 kb on + strand, within Pf1N1B4_37 at 42.529 kb on - strand, within Pf1N1B4_37 at 42.755 kb on + strand, within Pf1N1B4_37 at 42.911 kb on + strand at 42.912 kb on - strand at 42.952 kb on + strand at 42.952 kb on + strand at 42.952 kb on + strand at 42.952 kb on + strand at 42.952 kb on + strand at 42.952 kb on + strand at 42.952 kb on + strand at 42.953 kb on - strand at 42.953 kb on - strand at 42.953 kb on - strand at 42.953 kb on - strand at 42.953 kb on - strand at 42.953 kb on - strand at 42.954 kb on + strand at 43.269 kb on - strand, within Pf1N1B4_38 at 43.325 kb on + strand, within Pf1N1B4_38 at 43.325 kb on + strand, within Pf1N1B4_38 at 43.325 kb on + strand, within Pf1N1B4_38 at 43.326 kb on - strand at 43.326 kb on - strand, within Pf1N1B4_38 at 43.326 kb on - strand, within Pf1N1B4_38 at 43.355 kb on + strand, within Pf1N1B4_38 at 43.355 kb on + strand, within Pf1N1B4_38 at 43.355 kb on + strand, within Pf1N1B4_38 at 43.355 kb on + strand, within Pf1N1B4_38 at 43.355 kb on + strand, within Pf1N1B4_38 at 43.355 kb on + strand, within Pf1N1B4_38 at 43.356 kb on - strand, within Pf1N1B4_38 at 43.356 kb on - strand, within Pf1N1B4_38 at 43.440 kb on - strand at 43.482 kb on - strand, within Pf1N1B4_38 at 43.908 kb on + strand at 43.908 kb on + strand at 43.909 kb on - strand at 43.909 kb on - strand at 43.981 kb on - strand at 43.981 kb on - strand at 43.981 kb on - strand at 44.175 kb on + strand, within Pf1N1B4_39 at 44.176 kb on - strand, within Pf1N1B4_39 at 44.180 kb on - strand, within Pf1N1B4_39 at 44.180 kb on - strand, within Pf1N1B4_39 at 44.180 kb on - strand, within Pf1N1B4_39 at 44.180 kb on - strand, within Pf1N1B4_39 at 44.180 kb on - strand, within Pf1N1B4_39 at 44.180 kb on - strand at 44.180 kb on - strand, within Pf1N1B4_39 at 44.617 kb on - strand, within Pf1N1B4_39 at 44.617 kb on - strand, within Pf1N1B4_39 at 44.713 kb on - strand, within Pf1N1B4_39 at 44.748 kb on + strand, within Pf1N1B4_39 at 44.748 kb on + strand at 44.748 kb on + strand, within Pf1N1B4_39 at 44.749 kb on - strand, within Pf1N1B4_39
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 42,233 - Pf1N1B4_37 0.21 -1.2 42,275 + Pf1N1B4_37 0.26 +0.4 42,315 + Pf1N1B4_37 0.30 +0.8 42,316 - Pf1N1B4_37 0.31 -0.2 42,316 - Pf1N1B4_37 0.31 -0.2 42,369 + Pf1N1B4_37 0.37 -0.6 42,369 + Pf1N1B4_37 0.37 -0.6 42,369 + Pf1N1B4_37 0.37 +0.5 42,369 + Pf1N1B4_37 0.37 -2.0 42,370 - Pf1N1B4_37 0.37 -1.2 42,370 - Pf1N1B4_37 0.37 +1.8 42,370 - Pf1N1B4_37 0.37 -1.9 42,370 - Pf1N1B4_37 0.37 +0.2 42,411 + Pf1N1B4_37 0.42 +3.1 42,528 + Pf1N1B4_37 0.56 +1.2 42,529 - Pf1N1B4_37 0.56 +0.6 42,755 + Pf1N1B4_37 0.83 -0.2 42,911 + -1.3 42,912 - -1.4 42,952 + +0.1 42,952 + +0.0 42,952 + -1.2 42,952 + +0.7 42,952 + +0.4 42,952 + -0.6 42,952 + +1.0 42,953 - +0.8 42,953 - +1.2 42,953 - +0.8 42,953 - -1.2 42,953 - -0.1 42,953 - -0.2 42,954 + -0.8 43,269 - Pf1N1B4_38 0.32 +0.2 43,325 + Pf1N1B4_38 0.39 +0.4 43,325 + Pf1N1B4_38 0.39 +0.8 43,325 + Pf1N1B4_38 0.39 -0.2 43,326 - +1.1 43,326 - Pf1N1B4_38 0.39 +1.2 43,326 - Pf1N1B4_38 0.39 -0.2 43,355 + Pf1N1B4_38 0.43 +1.8 43,355 + Pf1N1B4_38 0.43 -1.2 43,355 + Pf1N1B4_38 0.43 +2.5 43,355 + Pf1N1B4_38 0.43 +2.4 43,355 + Pf1N1B4_38 0.43 +0.1 43,355 + Pf1N1B4_38 0.43 +0.7 43,356 - Pf1N1B4_38 0.43 -0.2 43,356 - Pf1N1B4_38 0.43 -0.4 43,440 - +1.8 43,482 - Pf1N1B4_38 0.59 +0.1 43,908 + -1.2 43,908 + -2.6 43,909 - -1.7 43,909 - +0.0 43,981 - -0.6 43,981 - -0.2 43,981 - -0.3 44,175 + Pf1N1B4_39 0.10 +1.5 44,176 - Pf1N1B4_39 0.10 -0.8 44,180 - Pf1N1B4_39 0.11 +1.2 44,180 - Pf1N1B4_39 0.11 +0.8 44,180 - Pf1N1B4_39 0.11 -0.4 44,180 - Pf1N1B4_39 0.11 +0.7 44,180 - Pf1N1B4_39 0.11 -1.0 44,180 - -0.8 44,180 - Pf1N1B4_39 0.11 -1.2 44,617 - Pf1N1B4_39 0.28 -0.2 44,617 - Pf1N1B4_39 0.28 -0.8 44,713 - Pf1N1B4_39 0.31 +0.0 44,748 + Pf1N1B4_39 0.33 +0.5 44,748 + +0.4 44,748 + Pf1N1B4_39 0.33 -2.0 44,749 - Pf1N1B4_39 0.33 -0.6
Or see this region's nucleotide sequence