Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_346 and Pf1N1B4_347 are separated by 120 nucleotides Pf1N1B4_347 and Pf1N1B4_348 are separated by 65 nucleotides Pf1N1B4_348 and Pf1N1B4_349 are separated by 28 nucleotides
Pf1N1B4_346 - SSU ribosomal protein S1p, at 409,382 to 411,073
_346
Pf1N1B4_347 - Cytidylate kinase (EC 2.7.4.25), at 411,194 to 411,814
_347
Pf1N1B4_348 - Cyclohexadienyl dehydrogenase (EC 1.3.1.12)(EC 1.3.1.43) / 5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19), at 411,880 to 414,087
_348
Pf1N1B4_349 - Biosynthetic Aromatic amino acid aminotransferase beta (EC 2.6.1.57), at 414,116 to 415,228
_349
Position (kb)
411
412
413
414
415 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 411.167 kb on - strand at 411.167 kb on - strand at 411.167 kb on - strand at 412.650 kb on - strand, within Pf1N1B4_348 at 412.650 kb on - strand, within Pf1N1B4_348 at 413.214 kb on - strand, within Pf1N1B4_348 at 413.214 kb on - strand, within Pf1N1B4_348 at 413.214 kb on - strand, within Pf1N1B4_348 at 413.282 kb on - strand, within Pf1N1B4_348 at 413.344 kb on - strand, within Pf1N1B4_348 at 413.344 kb on - strand, within Pf1N1B4_348 at 413.662 kb on - strand, within Pf1N1B4_348 at 413.662 kb on - strand, within Pf1N1B4_348 at 413.662 kb on - strand, within Pf1N1B4_348 at 413.662 kb on - strand, within Pf1N1B4_348 at 413.662 kb on - strand, within Pf1N1B4_348 at 413.951 kb on - strand at 413.951 kb on - strand at 414.087 kb on + strand at 414.088 kb on - strand at 414.088 kb on - strand at 414.088 kb on - strand at 414.088 kb on - strand at 414.088 kb on - strand at 414.088 kb on - strand at 414.088 kb on - strand at 414.088 kb on - strand at 414.088 kb on - strand at 414.088 kb on - strand at 414.088 kb on - strand at 414.088 kb on - strand at 414.088 kb on - strand at 414.088 kb on - strand at 414.211 kb on - strand at 414.396 kb on + strand, within Pf1N1B4_349 at 414.396 kb on + strand, within Pf1N1B4_349 at 414.397 kb on - strand, within Pf1N1B4_349 at 414.397 kb on - strand, within Pf1N1B4_349 at 414.535 kb on - strand, within Pf1N1B4_349 at 414.535 kb on - strand, within Pf1N1B4_349 at 414.585 kb on + strand, within Pf1N1B4_349 at 414.586 kb on - strand, within Pf1N1B4_349 at 414.622 kb on - strand, within Pf1N1B4_349 at 414.630 kb on + strand, within Pf1N1B4_349 at 414.631 kb on - strand, within Pf1N1B4_349 at 414.631 kb on - strand, within Pf1N1B4_349 at 414.631 kb on - strand, within Pf1N1B4_349 at 414.631 kb on - strand, within Pf1N1B4_349 at 414.852 kb on + strand, within Pf1N1B4_349 at 414.904 kb on - strand, within Pf1N1B4_349
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 411,167 - -1.5 411,167 - -0.3 411,167 - -0.9 412,650 - Pf1N1B4_348 0.35 -2.9 412,650 - Pf1N1B4_348 0.35 -1.7 413,214 - Pf1N1B4_348 0.60 +0.3 413,214 - Pf1N1B4_348 0.60 -0.1 413,214 - Pf1N1B4_348 0.60 -0.4 413,282 - Pf1N1B4_348 0.63 -0.3 413,344 - Pf1N1B4_348 0.66 -1.5 413,344 - Pf1N1B4_348 0.66 -0.7 413,662 - Pf1N1B4_348 0.81 -0.7 413,662 - Pf1N1B4_348 0.81 -1.5 413,662 - Pf1N1B4_348 0.81 -0.9 413,662 - Pf1N1B4_348 0.81 -1.5 413,662 - Pf1N1B4_348 0.81 +0.9 413,951 - +0.1 413,951 - -0.9 414,087 + -1.1 414,088 - -1.1 414,088 - -0.1 414,088 - -0.0 414,088 - +0.3 414,088 - +0.7 414,088 - +2.2 414,088 - +0.9 414,088 - +0.3 414,088 - +0.2 414,088 - +0.3 414,088 - +0.3 414,088 - -0.7 414,088 - -0.1 414,088 - +1.3 414,211 - +1.2 414,396 + Pf1N1B4_349 0.25 -0.7 414,396 + Pf1N1B4_349 0.25 +0.2 414,397 - Pf1N1B4_349 0.25 -0.6 414,397 - Pf1N1B4_349 0.25 +1.8 414,535 - Pf1N1B4_349 0.38 -0.6 414,535 - Pf1N1B4_349 0.38 -0.1 414,585 + Pf1N1B4_349 0.42 -1.3 414,586 - Pf1N1B4_349 0.42 -0.7 414,622 - Pf1N1B4_349 0.45 -0.7 414,630 + Pf1N1B4_349 0.46 -1.5 414,631 - Pf1N1B4_349 0.46 +1.6 414,631 - Pf1N1B4_349 0.46 -0.7 414,631 - Pf1N1B4_349 0.46 -1.1 414,631 - Pf1N1B4_349 0.46 -0.1 414,852 + Pf1N1B4_349 0.66 -1.3 414,904 - Pf1N1B4_349 0.71 +0.1
Or see this region's nucleotide sequence