Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3476 and Pf1N1B4_3477 are separated by 126 nucleotides Pf1N1B4_3477 and Pf1N1B4_3478 are separated by 491 nucleotides Pf1N1B4_3478 and Pf1N1B4_3479 overlap by 4 nucleotides
Pf1N1B4_3476 - Ribonucleotide reductase of class Ia (aerobic), alpha subunit (EC 1.17.4.1), at 3,881,300 to 3,884,194
_3476
Pf1N1B4_3477 - hypothetical protein, at 3,884,321 to 3,884,437
_3477
Pf1N1B4_3478 - Transcriptional regulatory protein RstA, at 3,884,929 to 3,885,651
_3478
Pf1N1B4_3479 - Sensor histidine kinase, at 3,885,648 to 3,887,258
_3479
Position (kb)
3884
3885
3886 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3884.268 kb on - strand at 3884.291 kb on - strand at 3884.308 kb on - strand at 3884.320 kb on - strand at 3884.498 kb on - strand at 3884.563 kb on - strand at 3884.563 kb on - strand at 3884.563 kb on - strand at 3884.569 kb on - strand at 3884.569 kb on - strand at 3884.591 kb on - strand at 3884.598 kb on - strand at 3884.625 kb on - strand at 3884.628 kb on - strand at 3884.680 kb on + strand at 3884.692 kb on + strand at 3884.693 kb on - strand at 3884.799 kb on + strand at 3884.830 kb on - strand at 3884.831 kb on + strand at 3884.832 kb on - strand at 3884.832 kb on - strand at 3884.832 kb on - strand at 3884.859 kb on - strand at 3884.875 kb on - strand at 3884.900 kb on + strand at 3884.998 kb on + strand at 3884.998 kb on + strand at 3884.999 kb on - strand at 3885.086 kb on + strand, within Pf1N1B4_3478 at 3885.151 kb on + strand, within Pf1N1B4_3478 at 3885.151 kb on + strand, within Pf1N1B4_3478 at 3885.151 kb on + strand, within Pf1N1B4_3478 at 3885.151 kb on + strand, within Pf1N1B4_3478 at 3885.151 kb on + strand, within Pf1N1B4_3478 at 3885.152 kb on - strand, within Pf1N1B4_3478 at 3885.152 kb on - strand, within Pf1N1B4_3478 at 3885.152 kb on - strand, within Pf1N1B4_3478 at 3885.152 kb on - strand, within Pf1N1B4_3478 at 3885.152 kb on - strand, within Pf1N1B4_3478 at 3885.152 kb on - strand, within Pf1N1B4_3478 at 3885.152 kb on - strand, within Pf1N1B4_3478 at 3885.152 kb on - strand, within Pf1N1B4_3478 at 3885.152 kb on - strand, within Pf1N1B4_3478 at 3885.232 kb on + strand, within Pf1N1B4_3478 at 3885.233 kb on - strand, within Pf1N1B4_3478 at 3885.233 kb on - strand, within Pf1N1B4_3478 at 3885.233 kb on - strand, within Pf1N1B4_3478 at 3885.233 kb on - strand, within Pf1N1B4_3478 at 3885.457 kb on - strand, within Pf1N1B4_3478 at 3885.476 kb on + strand, within Pf1N1B4_3478 at 3885.476 kb on + strand, within Pf1N1B4_3478 at 3885.477 kb on - strand, within Pf1N1B4_3478 at 3885.477 kb on - strand, within Pf1N1B4_3478 at 3885.672 kb on + strand at 3885.672 kb on + strand at 3885.672 kb on + strand at 3885.673 kb on - strand at 3886.068 kb on + strand, within Pf1N1B4_3479 at 3886.068 kb on + strand, within Pf1N1B4_3479 at 3886.068 kb on + strand, within Pf1N1B4_3479 at 3886.069 kb on - strand, within Pf1N1B4_3479 at 3886.069 kb on - strand, within Pf1N1B4_3479 at 3886.069 kb on - strand, within Pf1N1B4_3479 at 3886.320 kb on + strand, within Pf1N1B4_3479 at 3886.320 kb on + strand, within Pf1N1B4_3479 at 3886.320 kb on + strand, within Pf1N1B4_3479
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 3,884,268 - -0.4 3,884,291 - -1.9 3,884,308 - +1.3 3,884,320 - -0.2 3,884,498 - -0.8 3,884,563 - +1.9 3,884,563 - +0.9 3,884,563 - +0.3 3,884,569 - -3.1 3,884,569 - +0.5 3,884,591 - -1.1 3,884,598 - -1.1 3,884,625 - -0.7 3,884,628 - -0.7 3,884,680 + +0.2 3,884,692 + +0.4 3,884,693 - -0.6 3,884,799 + -0.1 3,884,830 - -0.7 3,884,831 + -1.4 3,884,832 - -0.1 3,884,832 - +0.5 3,884,832 - -0.3 3,884,859 - +0.9 3,884,875 - +0.2 3,884,900 + +0.6 3,884,998 + -0.8 3,884,998 + -1.2 3,884,999 - +0.6 3,885,086 + Pf1N1B4_3478 0.22 -0.1 3,885,151 + Pf1N1B4_3478 0.31 +0.2 3,885,151 + Pf1N1B4_3478 0.31 +0.5 3,885,151 + Pf1N1B4_3478 0.31 -0.6 3,885,151 + Pf1N1B4_3478 0.31 +0.6 3,885,151 + Pf1N1B4_3478 0.31 +0.9 3,885,152 - Pf1N1B4_3478 0.31 +1.0 3,885,152 - Pf1N1B4_3478 0.31 +1.9 3,885,152 - Pf1N1B4_3478 0.31 -0.4 3,885,152 - Pf1N1B4_3478 0.31 +1.0 3,885,152 - Pf1N1B4_3478 0.31 -0.7 3,885,152 - Pf1N1B4_3478 0.31 +1.3 3,885,152 - Pf1N1B4_3478 0.31 -1.3 3,885,152 - Pf1N1B4_3478 0.31 -0.7 3,885,152 - Pf1N1B4_3478 0.31 -1.2 3,885,232 + Pf1N1B4_3478 0.42 -0.1 3,885,233 - Pf1N1B4_3478 0.42 +0.9 3,885,233 - Pf1N1B4_3478 0.42 -2.1 3,885,233 - Pf1N1B4_3478 0.42 -0.0 3,885,233 - Pf1N1B4_3478 0.42 -0.1 3,885,457 - Pf1N1B4_3478 0.73 +1.4 3,885,476 + Pf1N1B4_3478 0.76 -0.7 3,885,476 + Pf1N1B4_3478 0.76 +1.3 3,885,477 - Pf1N1B4_3478 0.76 +0.6 3,885,477 - Pf1N1B4_3478 0.76 +0.1 3,885,672 + -1.3 3,885,672 + -0.1 3,885,672 + +0.3 3,885,673 - -0.4 3,886,068 + Pf1N1B4_3479 0.26 -0.4 3,886,068 + Pf1N1B4_3479 0.26 +0.6 3,886,068 + Pf1N1B4_3479 0.26 +1.3 3,886,069 - Pf1N1B4_3479 0.26 +0.2 3,886,069 - Pf1N1B4_3479 0.26 -1.1 3,886,069 - Pf1N1B4_3479 0.26 -0.4 3,886,320 + Pf1N1B4_3479 0.42 +0.4 3,886,320 + Pf1N1B4_3479 0.42 +0.9 3,886,320 + Pf1N1B4_3479 0.42 +0.3
Or see this region's nucleotide sequence