Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2425 and Pf1N1B4_2426 are separated by 114 nucleotides Pf1N1B4_2426 and Pf1N1B4_2427 are separated by 372 nucleotides Pf1N1B4_2427 and Pf1N1B4_2428 are separated by 13 nucleotides Pf1N1B4_2428 and Pf1N1B4_2429 are separated by 142 nucleotides
Pf1N1B4_2425 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 2,750,518 to 2,751,621
_2425
Pf1N1B4_2426 - FIG00953780: hypothetical protein, at 2,751,736 to 2,751,999
_2426
Pf1N1B4_2427 - Gamma-butyrobetaine,2-oxoglutarate dioxygenase (EC 1.14.11.1), at 2,752,372 to 2,753,535
_2427
Pf1N1B4_2428 - FIG143042: Thioesterase-like protein, at 2,753,549 to 2,754,025
_2428
Pf1N1B4_2429 - Carnitine 3-dehydrogenase (EC 1.1.1.108) (from data) , at 2,754,168 to 2,755,133
_2429
Position (kb)
2752
2753
2754 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2751.410 kb on + strand, within Pf1N1B4_2425 at 2751.458 kb on - strand, within Pf1N1B4_2425 at 2751.611 kb on + strand at 2751.686 kb on + strand at 2751.686 kb on + strand at 2751.686 kb on + strand at 2751.687 kb on - strand at 2751.687 kb on - strand at 2751.689 kb on - strand at 2751.734 kb on + strand at 2751.833 kb on + strand, within Pf1N1B4_2426 at 2751.833 kb on + strand, within Pf1N1B4_2426 at 2751.833 kb on + strand, within Pf1N1B4_2426 at 2751.833 kb on + strand, within Pf1N1B4_2426 at 2751.833 kb on + strand, within Pf1N1B4_2426 at 2751.834 kb on - strand, within Pf1N1B4_2426 at 2751.834 kb on - strand, within Pf1N1B4_2426 at 2751.834 kb on - strand, within Pf1N1B4_2426 at 2751.834 kb on - strand, within Pf1N1B4_2426 at 2751.834 kb on - strand, within Pf1N1B4_2426 at 2751.834 kb on - strand, within Pf1N1B4_2426 at 2751.849 kb on + strand, within Pf1N1B4_2426 at 2751.849 kb on + strand, within Pf1N1B4_2426 at 2751.850 kb on - strand, within Pf1N1B4_2426 at 2751.953 kb on - strand, within Pf1N1B4_2426 at 2752.092 kb on - strand at 2752.095 kb on + strand at 2752.095 kb on + strand at 2752.095 kb on + strand at 2752.095 kb on + strand at 2752.095 kb on + strand at 2752.095 kb on + strand at 2752.095 kb on + strand at 2752.096 kb on - strand at 2752.096 kb on - strand at 2752.096 kb on - strand at 2752.096 kb on - strand at 2752.096 kb on - strand at 2752.096 kb on - strand at 2752.096 kb on - strand at 2752.096 kb on - strand at 2752.096 kb on - strand at 2752.373 kb on + strand at 2752.421 kb on + strand at 2752.421 kb on + strand at 2752.421 kb on + strand at 2752.421 kb on + strand at 2752.421 kb on + strand at 2752.421 kb on + strand at 2752.421 kb on + strand at 2752.422 kb on - strand at 2752.422 kb on - strand at 2752.422 kb on - strand at 2752.422 kb on - strand at 2752.422 kb on - strand at 2752.923 kb on - strand, within Pf1N1B4_2427 at 2753.143 kb on + strand, within Pf1N1B4_2427 at 2753.144 kb on - strand, within Pf1N1B4_2427 at 2753.210 kb on + strand, within Pf1N1B4_2427 at 2753.210 kb on + strand, within Pf1N1B4_2427 at 2753.211 kb on - strand, within Pf1N1B4_2427 at 2753.366 kb on + strand, within Pf1N1B4_2427 at 2753.550 kb on + strand at 2753.684 kb on + strand, within Pf1N1B4_2428 at 2753.684 kb on + strand, within Pf1N1B4_2428 at 2753.684 kb on + strand at 2753.684 kb on + strand, within Pf1N1B4_2428 at 2753.685 kb on - strand, within Pf1N1B4_2428 at 2753.751 kb on + strand, within Pf1N1B4_2428 at 2753.832 kb on + strand, within Pf1N1B4_2428 at 2753.832 kb on + strand, within Pf1N1B4_2428 at 2753.832 kb on + strand, within Pf1N1B4_2428 at 2753.832 kb on + strand, within Pf1N1B4_2428 at 2753.833 kb on - strand, within Pf1N1B4_2428 at 2753.928 kb on + strand, within Pf1N1B4_2428 at 2753.946 kb on + strand, within Pf1N1B4_2428 at 2753.946 kb on + strand, within Pf1N1B4_2428 at 2753.946 kb on + strand, within Pf1N1B4_2428 at 2753.946 kb on + strand, within Pf1N1B4_2428 at 2753.946 kb on + strand, within Pf1N1B4_2428 at 2753.947 kb on - strand, within Pf1N1B4_2428 at 2753.947 kb on - strand, within Pf1N1B4_2428 at 2753.947 kb on - strand at 2754.006 kb on + strand at 2754.006 kb on + strand at 2754.007 kb on - strand at 2754.007 kb on - strand at 2754.007 kb on - strand at 2754.159 kb on + strand at 2754.159 kb on + strand at 2754.346 kb on + strand, within Pf1N1B4_2429 at 2754.346 kb on + strand, within Pf1N1B4_2429 at 2754.346 kb on + strand, within Pf1N1B4_2429 at 2754.346 kb on + strand, within Pf1N1B4_2429 at 2754.347 kb on - strand, within Pf1N1B4_2429 at 2754.347 kb on - strand, within Pf1N1B4_2429 at 2754.424 kb on + strand, within Pf1N1B4_2429 at 2754.424 kb on + strand, within Pf1N1B4_2429 at 2754.424 kb on + strand, within Pf1N1B4_2429 at 2754.425 kb on - strand, within Pf1N1B4_2429 at 2754.425 kb on - strand, within Pf1N1B4_2429 at 2754.425 kb on - strand, within Pf1N1B4_2429 at 2754.425 kb on - strand, within Pf1N1B4_2429 at 2754.438 kb on - strand, within Pf1N1B4_2429
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,751,410 + Pf1N1B4_2425 0.81 +0.7 2,751,458 - Pf1N1B4_2425 0.85 +1.1 2,751,611 + -0.8 2,751,686 + -1.3 2,751,686 + +0.0 2,751,686 + -1.3 2,751,687 - +0.3 2,751,687 - -1.5 2,751,689 - -0.5 2,751,734 + +0.6 2,751,833 + Pf1N1B4_2426 0.37 +1.9 2,751,833 + Pf1N1B4_2426 0.37 -0.5 2,751,833 + Pf1N1B4_2426 0.37 -0.3 2,751,833 + Pf1N1B4_2426 0.37 -1.3 2,751,833 + Pf1N1B4_2426 0.37 +1.0 2,751,834 - Pf1N1B4_2426 0.37 -0.1 2,751,834 - Pf1N1B4_2426 0.37 +0.6 2,751,834 - Pf1N1B4_2426 0.37 -1.3 2,751,834 - Pf1N1B4_2426 0.37 -1.3 2,751,834 - Pf1N1B4_2426 0.37 +0.7 2,751,834 - Pf1N1B4_2426 0.37 +0.5 2,751,849 + Pf1N1B4_2426 0.43 -1.1 2,751,849 + Pf1N1B4_2426 0.43 +0.3 2,751,850 - Pf1N1B4_2426 0.43 +2.3 2,751,953 - Pf1N1B4_2426 0.82 -0.3 2,752,092 - +0.9 2,752,095 + +0.3 2,752,095 + +1.3 2,752,095 + -1.0 2,752,095 + +0.6 2,752,095 + +0.3 2,752,095 + -0.9 2,752,095 + +1.3 2,752,096 - -1.0 2,752,096 - -0.3 2,752,096 - -0.3 2,752,096 - -0.7 2,752,096 - -0.1 2,752,096 - -0.5 2,752,096 - -0.3 2,752,096 - -0.4 2,752,096 - -0.3 2,752,373 + +0.5 2,752,421 + +1.3 2,752,421 + +0.2 2,752,421 + -0.3 2,752,421 + -1.0 2,752,421 + -0.0 2,752,421 + -1.0 2,752,421 + -0.3 2,752,422 - +1.3 2,752,422 - +0.3 2,752,422 - -0.7 2,752,422 - +0.0 2,752,422 - -1.5 2,752,923 - Pf1N1B4_2427 0.47 +0.0 2,753,143 + Pf1N1B4_2427 0.66 -1.7 2,753,144 - Pf1N1B4_2427 0.66 -1.2 2,753,210 + Pf1N1B4_2427 0.72 -2.5 2,753,210 + Pf1N1B4_2427 0.72 +0.3 2,753,211 - Pf1N1B4_2427 0.72 +1.1 2,753,366 + Pf1N1B4_2427 0.85 -1.5 2,753,550 + -1.3 2,753,684 + Pf1N1B4_2428 0.28 -0.5 2,753,684 + Pf1N1B4_2428 0.28 +0.6 2,753,684 + +0.3 2,753,684 + Pf1N1B4_2428 0.28 +0.3 2,753,685 - Pf1N1B4_2428 0.29 -0.3 2,753,751 + Pf1N1B4_2428 0.42 -1.0 2,753,832 + Pf1N1B4_2428 0.59 +0.5 2,753,832 + Pf1N1B4_2428 0.59 +0.1 2,753,832 + Pf1N1B4_2428 0.59 -0.1 2,753,832 + Pf1N1B4_2428 0.59 -0.5 2,753,833 - Pf1N1B4_2428 0.60 +0.5 2,753,928 + Pf1N1B4_2428 0.79 +1.3 2,753,946 + Pf1N1B4_2428 0.83 +0.1 2,753,946 + Pf1N1B4_2428 0.83 -0.2 2,753,946 + Pf1N1B4_2428 0.83 -2.1 2,753,946 + Pf1N1B4_2428 0.83 -0.5 2,753,946 + Pf1N1B4_2428 0.83 +0.5 2,753,947 - Pf1N1B4_2428 0.83 -1.7 2,753,947 - Pf1N1B4_2428 0.83 -0.4 2,753,947 - +1.9 2,754,006 + -1.8 2,754,006 + -0.9 2,754,007 - -0.8 2,754,007 - -0.3 2,754,007 - +0.3 2,754,159 + -1.2 2,754,159 + +0.2 2,754,346 + Pf1N1B4_2429 0.18 -0.4 2,754,346 + Pf1N1B4_2429 0.18 +0.3 2,754,346 + Pf1N1B4_2429 0.18 -1.7 2,754,346 + Pf1N1B4_2429 0.18 -1.3 2,754,347 - Pf1N1B4_2429 0.19 +0.8 2,754,347 - Pf1N1B4_2429 0.19 +0.9 2,754,424 + Pf1N1B4_2429 0.27 +1.3 2,754,424 + Pf1N1B4_2429 0.27 -0.3 2,754,424 + Pf1N1B4_2429 0.27 +1.1 2,754,425 - Pf1N1B4_2429 0.27 +0.3 2,754,425 - Pf1N1B4_2429 0.27 -1.0 2,754,425 - Pf1N1B4_2429 0.27 +0.1 2,754,425 - Pf1N1B4_2429 0.27 +0.0 2,754,438 - Pf1N1B4_2429 0.28 -0.1
Or see this region's nucleotide sequence