Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2412 and Pf1N1B4_2413 are separated by 183 nucleotides Pf1N1B4_2413 and Pf1N1B4_2414 are separated by 137 nucleotides
Pf1N1B4_2412 - ATP-dependent 23S rRNA helicase DbpA, at 2,732,340 to 2,733,725
_2412
Pf1N1B4_2413 - Permeases of the major facilitator superfamily, at 2,733,909 to 2,735,336
_2413
Pf1N1B4_2414 - TldE/PmbA family protein, Beta/Gamma-proteobacterial subgroup, at 2,735,474 to 2,736,823
_2414
Position (kb)
2733
2734
2735
2736 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2733.055 kb on + strand, within Pf1N1B4_2412 at 2733.055 kb on + strand, within Pf1N1B4_2412 at 2733.055 kb on + strand, within Pf1N1B4_2412 at 2733.056 kb on - strand, within Pf1N1B4_2412 at 2733.227 kb on - strand, within Pf1N1B4_2412 at 2733.698 kb on + strand at 2733.698 kb on + strand at 2733.701 kb on + strand at 2733.701 kb on + strand at 2733.702 kb on - strand at 2733.702 kb on - strand at 2733.702 kb on - strand at 2733.760 kb on + strand at 2733.760 kb on + strand at 2733.760 kb on + strand at 2733.761 kb on - strand at 2733.761 kb on - strand at 2733.790 kb on - strand at 2733.800 kb on - strand at 2733.807 kb on - strand at 2734.846 kb on + strand, within Pf1N1B4_2413 at 2734.846 kb on + strand, within Pf1N1B4_2413 at 2734.846 kb on + strand at 2734.847 kb on - strand, within Pf1N1B4_2413 at 2735.145 kb on + strand, within Pf1N1B4_2413 at 2735.164 kb on + strand, within Pf1N1B4_2413 at 2735.164 kb on + strand, within Pf1N1B4_2413 at 2735.164 kb on + strand, within Pf1N1B4_2413 at 2735.164 kb on + strand, within Pf1N1B4_2413 at 2735.164 kb on + strand, within Pf1N1B4_2413 at 2735.165 kb on - strand, within Pf1N1B4_2413 at 2735.165 kb on - strand, within Pf1N1B4_2413 at 2735.476 kb on - strand at 2735.488 kb on + strand at 2735.489 kb on - strand at 2735.489 kb on - strand at 2735.489 kb on - strand at 2735.544 kb on + strand at 2735.544 kb on + strand at 2735.544 kb on + strand at 2735.544 kb on + strand at 2735.544 kb on + strand at 2735.545 kb on - strand at 2735.545 kb on - strand at 2735.545 kb on - strand at 2735.545 kb on - strand at 2735.545 kb on - strand at 2735.545 kb on - strand at 2735.545 kb on - strand at 2735.545 kb on - strand at 2735.545 kb on - strand at 2735.613 kb on + strand, within Pf1N1B4_2414 at 2735.613 kb on + strand, within Pf1N1B4_2414 at 2735.613 kb on + strand, within Pf1N1B4_2414 at 2735.614 kb on - strand, within Pf1N1B4_2414 at 2735.727 kb on + strand, within Pf1N1B4_2414 at 2735.728 kb on - strand, within Pf1N1B4_2414 at 2735.736 kb on + strand, within Pf1N1B4_2414 at 2735.737 kb on - strand, within Pf1N1B4_2414 at 2735.754 kb on + strand, within Pf1N1B4_2414 at 2735.754 kb on + strand, within Pf1N1B4_2414 at 2735.754 kb on + strand, within Pf1N1B4_2414 at 2735.754 kb on + strand, within Pf1N1B4_2414 at 2735.754 kb on + strand, within Pf1N1B4_2414 at 2735.754 kb on + strand, within Pf1N1B4_2414 at 2735.755 kb on - strand, within Pf1N1B4_2414 at 2735.755 kb on - strand, within Pf1N1B4_2414 at 2735.865 kb on + strand, within Pf1N1B4_2414 at 2735.999 kb on + strand, within Pf1N1B4_2414 at 2735.999 kb on + strand, within Pf1N1B4_2414 at 2735.999 kb on + strand, within Pf1N1B4_2414 at 2735.999 kb on + strand, within Pf1N1B4_2414 at 2735.999 kb on + strand, within Pf1N1B4_2414 at 2735.999 kb on + strand, within Pf1N1B4_2414 at 2735.999 kb on + strand, within Pf1N1B4_2414 at 2736.000 kb on - strand, within Pf1N1B4_2414 at 2736.000 kb on - strand, within Pf1N1B4_2414 at 2736.000 kb on - strand, within Pf1N1B4_2414 at 2736.000 kb on - strand, within Pf1N1B4_2414 at 2736.000 kb on - strand, within Pf1N1B4_2414 at 2736.249 kb on + strand, within Pf1N1B4_2414 at 2736.250 kb on - strand, within Pf1N1B4_2414 at 2736.250 kb on - strand, within Pf1N1B4_2414 at 2736.250 kb on - strand, within Pf1N1B4_2414 at 2736.250 kb on - strand, within Pf1N1B4_2414
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,733,055 + Pf1N1B4_2412 0.52 -1.5 2,733,055 + Pf1N1B4_2412 0.52 +2.3 2,733,055 + Pf1N1B4_2412 0.52 -1.2 2,733,056 - Pf1N1B4_2412 0.52 -0.2 2,733,227 - Pf1N1B4_2412 0.64 +1.9 2,733,698 + +1.3 2,733,698 + +0.5 2,733,701 + +0.3 2,733,701 + +0.1 2,733,702 - -1.4 2,733,702 - -0.4 2,733,702 - -1.0 2,733,760 + -1.2 2,733,760 + -0.2 2,733,760 + +0.3 2,733,761 - +0.5 2,733,761 - -0.5 2,733,790 - +0.6 2,733,800 - -0.4 2,733,807 - +0.9 2,734,846 + Pf1N1B4_2413 0.66 -1.0 2,734,846 + Pf1N1B4_2413 0.66 +2.3 2,734,846 + +0.3 2,734,847 - Pf1N1B4_2413 0.66 -0.1 2,735,145 + Pf1N1B4_2413 0.87 +1.0 2,735,164 + Pf1N1B4_2413 0.88 +0.1 2,735,164 + Pf1N1B4_2413 0.88 +0.8 2,735,164 + Pf1N1B4_2413 0.88 -0.4 2,735,164 + Pf1N1B4_2413 0.88 +0.0 2,735,164 + Pf1N1B4_2413 0.88 -0.4 2,735,165 - Pf1N1B4_2413 0.88 +1.8 2,735,165 - Pf1N1B4_2413 0.88 +0.8 2,735,476 - +0.1 2,735,488 + +0.3 2,735,489 - -0.2 2,735,489 - -0.9 2,735,489 - -1.7 2,735,544 + -1.6 2,735,544 + -0.2 2,735,544 + -0.3 2,735,544 + -0.2 2,735,544 + -1.5 2,735,545 - +0.3 2,735,545 - +3.3 2,735,545 - -0.1 2,735,545 - +0.0 2,735,545 - -2.7 2,735,545 - -1.0 2,735,545 - -0.8 2,735,545 - +2.1 2,735,545 - -1.0 2,735,613 + Pf1N1B4_2414 0.10 +0.0 2,735,613 + Pf1N1B4_2414 0.10 +1.1 2,735,613 + Pf1N1B4_2414 0.10 -1.5 2,735,614 - Pf1N1B4_2414 0.10 -1.0 2,735,727 + Pf1N1B4_2414 0.19 +0.0 2,735,728 - Pf1N1B4_2414 0.19 -1.2 2,735,736 + Pf1N1B4_2414 0.19 -0.2 2,735,737 - Pf1N1B4_2414 0.19 -0.7 2,735,754 + Pf1N1B4_2414 0.21 -0.5 2,735,754 + Pf1N1B4_2414 0.21 +0.1 2,735,754 + Pf1N1B4_2414 0.21 +0.3 2,735,754 + Pf1N1B4_2414 0.21 -1.0 2,735,754 + Pf1N1B4_2414 0.21 +0.5 2,735,754 + Pf1N1B4_2414 0.21 +1.3 2,735,755 - Pf1N1B4_2414 0.21 -0.4 2,735,755 - Pf1N1B4_2414 0.21 -1.2 2,735,865 + Pf1N1B4_2414 0.29 -0.2 2,735,999 + Pf1N1B4_2414 0.39 -0.7 2,735,999 + Pf1N1B4_2414 0.39 -0.1 2,735,999 + Pf1N1B4_2414 0.39 -0.8 2,735,999 + Pf1N1B4_2414 0.39 -0.2 2,735,999 + Pf1N1B4_2414 0.39 +0.0 2,735,999 + Pf1N1B4_2414 0.39 -0.4 2,735,999 + Pf1N1B4_2414 0.39 +0.9 2,736,000 - Pf1N1B4_2414 0.39 +0.8 2,736,000 - Pf1N1B4_2414 0.39 +0.8 2,736,000 - Pf1N1B4_2414 0.39 +1.3 2,736,000 - Pf1N1B4_2414 0.39 +1.2 2,736,000 - Pf1N1B4_2414 0.39 +0.0 2,736,249 + Pf1N1B4_2414 0.57 +0.6 2,736,250 - Pf1N1B4_2414 0.57 +1.7 2,736,250 - Pf1N1B4_2414 0.57 +1.3 2,736,250 - Pf1N1B4_2414 0.57 +0.6 2,736,250 - Pf1N1B4_2414 0.57 -0.1
Or see this region's nucleotide sequence