Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_216

Experiment: LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_215 and Pf1N1B4_216 are separated by 381 nucleotidesPf1N1B4_216 and Pf1N1B4_217 are separated by 132 nucleotides Pf1N1B4_215 - Pyoverdine chromophore precursor synthetase PvdL, at 267,867 to 275,390 _215 Pf1N1B4_216 - Sigma factor PvdS, controling pyoverdin biosynthesis, at 275,772 to 276,320 _216 Pf1N1B4_217 - Hypothetical protein PvdY @ Siderophore synthetase small component, acetyltransferase, at 276,453 to 277,466 _217 Position (kb) 275 276 277Strain fitness (log2 ratio) -2 -1 0 1 2 3at 274.888 kb on + strandat 274.888 kb on + strandat 274.888 kb on + strandat 275.134 kb on + strandat 275.161 kb on + strandat 275.162 kb on - strandat 275.563 kb on + strandat 275.564 kb on - strandat 275.564 kb on - strandat 275.564 kb on - strandat 275.606 kb on + strandat 275.607 kb on - strandat 275.714 kb on - strandat 275.749 kb on + strandat 275.750 kb on - strandat 275.813 kb on - strandat 275.967 kb on + strand, within Pf1N1B4_216at 275.967 kb on + strand, within Pf1N1B4_216at 275.967 kb on + strand, within Pf1N1B4_216at 275.967 kb on + strand, within Pf1N1B4_216at 275.967 kb on + strand, within Pf1N1B4_216at 275.968 kb on - strand, within Pf1N1B4_216at 276.177 kb on + strand, within Pf1N1B4_216at 276.177 kb on + strandat 276.346 kb on + strandat 276.363 kb on + strandat 276.363 kb on + strandat 276.364 kb on - strandat 276.368 kb on + strandat 276.368 kb on + strandat 276.368 kb on + strandat 276.369 kb on - strandat 276.392 kb on - strandat 276.392 kb on - strandat 276.640 kb on + strand, within Pf1N1B4_217at 276.641 kb on - strand, within Pf1N1B4_217at 276.641 kb on - strand, within Pf1N1B4_217at 276.743 kb on - strand, within Pf1N1B4_217at 276.743 kb on - strand, within Pf1N1B4_217at 276.743 kb on - strandat 276.743 kb on - strand, within Pf1N1B4_217at 276.743 kb on - strandat 276.743 kb on - strand, within Pf1N1B4_217at 276.775 kb on + strand, within Pf1N1B4_217at 276.776 kb on - strand, within Pf1N1B4_217at 276.776 kb on - strand, within Pf1N1B4_217at 276.787 kb on + strand, within Pf1N1B4_217at 276.788 kb on - strand, within Pf1N1B4_217at 276.788 kb on - strand, within Pf1N1B4_217at 276.831 kb on - strandat 276.831 kb on - strand, within Pf1N1B4_217at 276.860 kb on - strand, within Pf1N1B4_217at 276.961 kb on - strand, within Pf1N1B4_217at 277.081 kb on + strand, within Pf1N1B4_217

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Per-strain Table

Position Strand Gene LocusTag Fraction LB
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274,888 + +1.2
274,888 + -0.1
274,888 + -0.5
275,134 + -0.2
275,161 + -1.0
275,162 - -0.8
275,563 + +2.1
275,564 - +0.8
275,564 - +1.3
275,564 - -0.6
275,606 + -1.0
275,607 - +0.2
275,714 - -0.6
275,749 + -0.8
275,750 - +0.1
275,813 - -1.2
275,967 + Pf1N1B4_216 0.36 -0.8
275,967 + Pf1N1B4_216 0.36 -1.8
275,967 + Pf1N1B4_216 0.36 +0.8
275,967 + Pf1N1B4_216 0.36 -1.8
275,967 + Pf1N1B4_216 0.36 -1.4
275,968 - Pf1N1B4_216 0.36 -0.5
276,177 + Pf1N1B4_216 0.74 -0.6
276,177 + -0.2
276,346 + -0.1
276,363 + +2.8
276,363 + +0.2
276,364 - -1.2
276,368 + -1.2
276,368 + -2.2
276,368 + +0.0
276,369 - -0.2
276,392 - -1.4
276,392 - +0.3
276,640 + Pf1N1B4_217 0.18 -1.0
276,641 - Pf1N1B4_217 0.19 +0.8
276,641 - Pf1N1B4_217 0.19 +0.2
276,743 - Pf1N1B4_217 0.29 -0.0
276,743 - Pf1N1B4_217 0.29 +1.2
276,743 - +0.5
276,743 - Pf1N1B4_217 0.29 -2.4
276,743 - +0.4
276,743 - Pf1N1B4_217 0.29 +0.3
276,775 + Pf1N1B4_217 0.32 -2.0
276,776 - Pf1N1B4_217 0.32 +0.4
276,776 - Pf1N1B4_217 0.32 +1.2
276,787 + Pf1N1B4_217 0.33 +0.2
276,788 - Pf1N1B4_217 0.33 -0.4
276,788 - Pf1N1B4_217 0.33 +0.1
276,831 - +1.4
276,831 - Pf1N1B4_217 0.37 +1.1
276,860 - Pf1N1B4_217 0.40 -0.9
276,961 - Pf1N1B4_217 0.50 -1.6
277,081 + Pf1N1B4_217 0.62 +1.3

Or see this region's nucleotide sequence