Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2149 and Pf1N1B4_2150 are separated by 127 nucleotides Pf1N1B4_2150 and Pf1N1B4_2151 are separated by 69 nucleotides
Pf1N1B4_2149 - Acetyl-CoA hydrolase, at 2,458,747 to 2,460,669
_2149
Pf1N1B4_2150 - Xanthine phosphoribosyltransferase (EC 2.4.2.22), at 2,460,797 to 2,461,369
_2150
Pf1N1B4_2151 - ATP-dependent DNA helicase Rep, at 2,461,439 to 2,463,448
_2151
Position (kb)
2460
2461
2462 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2459.842 kb on + strand, within Pf1N1B4_2149 at 2459.842 kb on + strand, within Pf1N1B4_2149 at 2459.872 kb on - strand, within Pf1N1B4_2149 at 2459.872 kb on - strand, within Pf1N1B4_2149 at 2459.905 kb on + strand, within Pf1N1B4_2149 at 2459.905 kb on + strand, within Pf1N1B4_2149 at 2459.905 kb on + strand, within Pf1N1B4_2149 at 2459.905 kb on + strand, within Pf1N1B4_2149 at 2459.906 kb on - strand, within Pf1N1B4_2149 at 2459.906 kb on - strand, within Pf1N1B4_2149 at 2459.906 kb on - strand, within Pf1N1B4_2149 at 2459.906 kb on - strand, within Pf1N1B4_2149 at 2459.906 kb on - strand, within Pf1N1B4_2149 at 2459.920 kb on + strand, within Pf1N1B4_2149 at 2460.058 kb on + strand, within Pf1N1B4_2149 at 2460.058 kb on + strand, within Pf1N1B4_2149 at 2460.058 kb on + strand, within Pf1N1B4_2149 at 2460.058 kb on + strand, within Pf1N1B4_2149 at 2460.059 kb on - strand, within Pf1N1B4_2149 at 2460.059 kb on - strand, within Pf1N1B4_2149 at 2460.059 kb on - strand, within Pf1N1B4_2149 at 2460.059 kb on - strand, within Pf1N1B4_2149 at 2460.059 kb on - strand, within Pf1N1B4_2149 at 2460.059 kb on - strand, within Pf1N1B4_2149 at 2460.059 kb on - strand, within Pf1N1B4_2149 at 2460.167 kb on - strand, within Pf1N1B4_2149 at 2460.470 kb on + strand, within Pf1N1B4_2149 at 2460.470 kb on + strand at 2460.470 kb on + strand, within Pf1N1B4_2149 at 2460.470 kb on + strand, within Pf1N1B4_2149 at 2460.471 kb on - strand, within Pf1N1B4_2149 at 2460.471 kb on - strand, within Pf1N1B4_2149 at 2460.471 kb on - strand, within Pf1N1B4_2149 at 2460.471 kb on - strand, within Pf1N1B4_2149 at 2460.471 kb on - strand, within Pf1N1B4_2149 at 2460.667 kb on + strand at 2460.667 kb on + strand at 2460.744 kb on + strand at 2460.744 kb on + strand at 2460.745 kb on - strand at 2460.745 kb on - strand at 2460.745 kb on - strand at 2460.813 kb on - strand at 2460.917 kb on - strand, within Pf1N1B4_2150 at 2461.080 kb on + strand, within Pf1N1B4_2150 at 2461.081 kb on - strand, within Pf1N1B4_2150 at 2461.081 kb on - strand, within Pf1N1B4_2150 at 2461.382 kb on + strand at 2461.408 kb on + strand at 2461.430 kb on + strand at 2461.449 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,459,842 + Pf1N1B4_2149 0.57 +0.5 2,459,842 + Pf1N1B4_2149 0.57 +0.3 2,459,872 - Pf1N1B4_2149 0.59 +0.7 2,459,872 - Pf1N1B4_2149 0.59 -0.1 2,459,905 + Pf1N1B4_2149 0.60 -0.3 2,459,905 + Pf1N1B4_2149 0.60 -0.9 2,459,905 + Pf1N1B4_2149 0.60 -0.0 2,459,905 + Pf1N1B4_2149 0.60 +0.9 2,459,906 - Pf1N1B4_2149 0.60 -1.2 2,459,906 - Pf1N1B4_2149 0.60 +0.5 2,459,906 - Pf1N1B4_2149 0.60 -0.5 2,459,906 - Pf1N1B4_2149 0.60 -0.5 2,459,906 - Pf1N1B4_2149 0.60 -0.5 2,459,920 + Pf1N1B4_2149 0.61 -0.9 2,460,058 + Pf1N1B4_2149 0.68 -0.1 2,460,058 + Pf1N1B4_2149 0.68 -0.4 2,460,058 + Pf1N1B4_2149 0.68 -1.5 2,460,058 + Pf1N1B4_2149 0.68 +0.1 2,460,059 - Pf1N1B4_2149 0.68 -0.1 2,460,059 - Pf1N1B4_2149 0.68 -0.5 2,460,059 - Pf1N1B4_2149 0.68 +0.3 2,460,059 - Pf1N1B4_2149 0.68 +0.3 2,460,059 - Pf1N1B4_2149 0.68 -0.7 2,460,059 - Pf1N1B4_2149 0.68 -2.9 2,460,059 - Pf1N1B4_2149 0.68 +0.9 2,460,167 - Pf1N1B4_2149 0.74 -1.1 2,460,470 + Pf1N1B4_2149 0.90 -0.0 2,460,470 + +0.5 2,460,470 + Pf1N1B4_2149 0.90 -0.1 2,460,470 + Pf1N1B4_2149 0.90 -0.0 2,460,471 - Pf1N1B4_2149 0.90 +0.6 2,460,471 - Pf1N1B4_2149 0.90 -0.5 2,460,471 - Pf1N1B4_2149 0.90 +0.1 2,460,471 - Pf1N1B4_2149 0.90 -0.1 2,460,471 - Pf1N1B4_2149 0.90 -0.2 2,460,667 + +0.7 2,460,667 + +0.6 2,460,744 + +0.5 2,460,744 + -0.2 2,460,745 - +0.0 2,460,745 - -1.4 2,460,745 - +0.3 2,460,813 - +0.5 2,460,917 - Pf1N1B4_2150 0.21 +0.0 2,461,080 + Pf1N1B4_2150 0.49 -2.0 2,461,081 - Pf1N1B4_2150 0.50 -0.9 2,461,081 - Pf1N1B4_2150 0.50 -1.0 2,461,382 + +0.5 2,461,408 + -1.0 2,461,430 + -0.0 2,461,449 - +0.1
Or see this region's nucleotide sequence