Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1711 and Pf1N1B4_1712 overlap by 4 nucleotides Pf1N1B4_1712 and Pf1N1B4_1713 overlap by 20 nucleotides Pf1N1B4_1713 and Pf1N1B4_1714 are separated by 474 nucleotides
Pf1N1B4_1711 - ABC-type polar amino acid transport system, ATPase component, at 1,959,272 to 1,960,054
_1711
Pf1N1B4_1712 - Glutamate transport membrane-spanning protein, at 1,960,051 to 1,960,710
_1712
Pf1N1B4_1713 - Glutamate transport membrane-spanning protein, at 1,960,691 to 1,961,356
_1713
Pf1N1B4_1714 - Methionine ABC transporter substrate-binding protein, at 1,961,831 to 1,962,613
_1714
Position (kb)
1960
1961
1962 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1960.104 kb on - strand at 1960.104 kb on - strand at 1960.129 kb on + strand, within Pf1N1B4_1712 at 1960.139 kb on + strand, within Pf1N1B4_1712 at 1960.140 kb on - strand, within Pf1N1B4_1712 at 1960.157 kb on + strand, within Pf1N1B4_1712 at 1960.157 kb on + strand, within Pf1N1B4_1712 at 1960.158 kb on - strand, within Pf1N1B4_1712 at 1960.158 kb on - strand, within Pf1N1B4_1712 at 1960.158 kb on - strand, within Pf1N1B4_1712 at 1960.158 kb on - strand, within Pf1N1B4_1712 at 1960.158 kb on - strand, within Pf1N1B4_1712 at 1960.158 kb on - strand at 1960.158 kb on - strand, within Pf1N1B4_1712 at 1960.247 kb on + strand, within Pf1N1B4_1712 at 1960.292 kb on - strand, within Pf1N1B4_1712 at 1960.292 kb on - strand, within Pf1N1B4_1712 at 1960.302 kb on - strand, within Pf1N1B4_1712 at 1960.308 kb on - strand, within Pf1N1B4_1712 at 1960.494 kb on - strand, within Pf1N1B4_1712 at 1960.494 kb on - strand, within Pf1N1B4_1712 at 1960.494 kb on - strand, within Pf1N1B4_1712 at 1960.494 kb on - strand, within Pf1N1B4_1712 at 1960.536 kb on - strand, within Pf1N1B4_1712 at 1960.536 kb on - strand, within Pf1N1B4_1712 at 1960.589 kb on + strand, within Pf1N1B4_1712 at 1960.589 kb on + strand, within Pf1N1B4_1712 at 1960.590 kb on - strand, within Pf1N1B4_1712 at 1960.594 kb on + strand, within Pf1N1B4_1712 at 1960.594 kb on + strand, within Pf1N1B4_1712 at 1960.594 kb on + strand, within Pf1N1B4_1712 at 1960.812 kb on + strand, within Pf1N1B4_1713 at 1960.812 kb on + strand, within Pf1N1B4_1713 at 1960.812 kb on + strand, within Pf1N1B4_1713 at 1960.813 kb on - strand, within Pf1N1B4_1713 at 1960.824 kb on + strand, within Pf1N1B4_1713 at 1960.839 kb on + strand, within Pf1N1B4_1713 at 1960.839 kb on + strand, within Pf1N1B4_1713 at 1960.840 kb on - strand, within Pf1N1B4_1713 at 1960.954 kb on - strand, within Pf1N1B4_1713 at 1960.954 kb on - strand, within Pf1N1B4_1713 at 1960.954 kb on - strand, within Pf1N1B4_1713 at 1961.052 kb on + strand, within Pf1N1B4_1713 at 1961.052 kb on + strand, within Pf1N1B4_1713 at 1961.052 kb on + strand, within Pf1N1B4_1713 at 1961.184 kb on + strand, within Pf1N1B4_1713 at 1961.184 kb on + strand, within Pf1N1B4_1713 at 1961.185 kb on - strand, within Pf1N1B4_1713 at 1961.185 kb on - strand, within Pf1N1B4_1713 at 1961.209 kb on - strand, within Pf1N1B4_1713 at 1961.344 kb on - strand at 1961.369 kb on - strand at 1961.369 kb on - strand at 1961.470 kb on - strand at 1961.498 kb on - strand at 1961.532 kb on + strand at 1961.533 kb on - strand at 1961.533 kb on - strand at 1961.550 kb on - strand at 1961.572 kb on + strand at 1961.572 kb on + strand at 1961.572 kb on + strand at 1961.572 kb on + strand at 1961.573 kb on - strand at 1961.650 kb on + strand at 1961.650 kb on + strand at 1961.650 kb on + strand at 1961.761 kb on + strand at 1961.781 kb on - strand at 1961.859 kb on + strand at 1961.859 kb on + strand at 1961.860 kb on - strand at 1961.860 kb on - strand at 1961.860 kb on - strand at 1961.968 kb on - strand, within Pf1N1B4_1714 at 1961.968 kb on - strand, within Pf1N1B4_1714 at 1962.033 kb on + strand, within Pf1N1B4_1714 at 1962.034 kb on - strand, within Pf1N1B4_1714
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,960,104 - +0.6 1,960,104 - -0.6 1,960,129 + Pf1N1B4_1712 0.12 -0.0 1,960,139 + Pf1N1B4_1712 0.13 -0.1 1,960,140 - Pf1N1B4_1712 0.13 -0.9 1,960,157 + Pf1N1B4_1712 0.16 -0.0 1,960,157 + Pf1N1B4_1712 0.16 +0.7 1,960,158 - Pf1N1B4_1712 0.16 +0.4 1,960,158 - Pf1N1B4_1712 0.16 +0.2 1,960,158 - Pf1N1B4_1712 0.16 +0.3 1,960,158 - Pf1N1B4_1712 0.16 +0.6 1,960,158 - Pf1N1B4_1712 0.16 -0.4 1,960,158 - +1.4 1,960,158 - Pf1N1B4_1712 0.16 -1.7 1,960,247 + Pf1N1B4_1712 0.30 +1.4 1,960,292 - Pf1N1B4_1712 0.37 +0.6 1,960,292 - Pf1N1B4_1712 0.37 +0.4 1,960,302 - Pf1N1B4_1712 0.38 -0.9 1,960,308 - Pf1N1B4_1712 0.39 +1.4 1,960,494 - Pf1N1B4_1712 0.67 +1.4 1,960,494 - Pf1N1B4_1712 0.67 -0.3 1,960,494 - Pf1N1B4_1712 0.67 -2.2 1,960,494 - Pf1N1B4_1712 0.67 +0.1 1,960,536 - Pf1N1B4_1712 0.73 -1.2 1,960,536 - Pf1N1B4_1712 0.73 -0.3 1,960,589 + Pf1N1B4_1712 0.82 +0.8 1,960,589 + Pf1N1B4_1712 0.82 -0.7 1,960,590 - Pf1N1B4_1712 0.82 -1.6 1,960,594 + Pf1N1B4_1712 0.82 +1.0 1,960,594 + Pf1N1B4_1712 0.82 +1.6 1,960,594 + Pf1N1B4_1712 0.82 +0.4 1,960,812 + Pf1N1B4_1713 0.18 -0.2 1,960,812 + Pf1N1B4_1713 0.18 +0.8 1,960,812 + Pf1N1B4_1713 0.18 -0.3 1,960,813 - Pf1N1B4_1713 0.18 -0.2 1,960,824 + Pf1N1B4_1713 0.20 -0.2 1,960,839 + Pf1N1B4_1713 0.22 +1.4 1,960,839 + Pf1N1B4_1713 0.22 +0.7 1,960,840 - Pf1N1B4_1713 0.22 +0.1 1,960,954 - Pf1N1B4_1713 0.39 +0.2 1,960,954 - Pf1N1B4_1713 0.39 -0.6 1,960,954 - Pf1N1B4_1713 0.39 +0.9 1,961,052 + Pf1N1B4_1713 0.54 +0.4 1,961,052 + Pf1N1B4_1713 0.54 -0.6 1,961,052 + Pf1N1B4_1713 0.54 +1.4 1,961,184 + Pf1N1B4_1713 0.74 +0.5 1,961,184 + Pf1N1B4_1713 0.74 +0.4 1,961,185 - Pf1N1B4_1713 0.74 -0.7 1,961,185 - Pf1N1B4_1713 0.74 -0.2 1,961,209 - Pf1N1B4_1713 0.78 -1.2 1,961,344 - -0.1 1,961,369 - -0.6 1,961,369 - -1.6 1,961,470 - +0.7 1,961,498 - +0.2 1,961,532 + +0.4 1,961,533 - +1.4 1,961,533 - -0.9 1,961,550 - +1.8 1,961,572 + -0.6 1,961,572 + -0.3 1,961,572 + -0.0 1,961,572 + +1.4 1,961,573 - -0.3 1,961,650 + +0.4 1,961,650 + +1.4 1,961,650 + +0.1 1,961,761 + +0.4 1,961,781 - +0.6 1,961,859 + +0.3 1,961,859 + -1.2 1,961,860 - -1.4 1,961,860 - -0.2 1,961,860 - +0.4 1,961,968 - Pf1N1B4_1714 0.17 +0.4 1,961,968 - Pf1N1B4_1714 0.17 -0.3 1,962,033 + Pf1N1B4_1714 0.26 -1.1 1,962,034 - Pf1N1B4_1714 0.26 -0.9
Or see this region's nucleotide sequence