Experiment: LB 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
     
    
        
         
     
    
        
         
     
    
        
         
     
    
         
     
     
 
500 nt Pf1N1B4_1228 and Pf1N1B4_1229 are separated by 10 nucleotides Pf1N1B4_1229 and Pf1N1B4_1230 are separated by 161 nucleotides  
        
        Pf1N1B4_1228 - 3-hydroxyisobutyrate dehydrogenase (EC 1.1.1.31), at 1,394,068 to 1,394,955 
         
        _1228 
         
        
        Pf1N1B4_1229 - Methylmalonate-semialdehyde dehydrogenase (EC 1.2.1.27) (from data) , at 1,394,966 to 1,396,483 
         
        _1229 
         
        
        Pf1N1B4_1230 - Transcriptional regulator, LysR family, at 1,396,645 to 1,397,592 
         
        _1230 
         Position (kb)  
1394 
 
1395 
 
1396 
 
1397 Strain fitness (log2 ratio)  
-2 
 
-1 
 
0 
 
1 
 
2 at 1393.978 kb on - strand at 1393.978 kb on - strand at 1394.144 kb on + strand at 1394.144 kb on + strand at 1394.144 kb on + strand at 1394.144 kb on + strand at 1394.145 kb on - strand at 1394.145 kb on - strand at 1394.303 kb on + strand, within Pf1N1B4_1228 at 1394.303 kb on + strand, within Pf1N1B4_1228 at 1394.303 kb on + strand, within Pf1N1B4_1228 at 1394.303 kb on + strand at 1394.303 kb on + strand, within Pf1N1B4_1228 at 1394.304 kb on - strand, within Pf1N1B4_1228 at 1394.304 kb on - strand, within Pf1N1B4_1228 at 1394.304 kb on - strand, within Pf1N1B4_1228 at 1394.304 kb on - strand, within Pf1N1B4_1228 at 1394.304 kb on - strand, within Pf1N1B4_1228 at 1394.304 kb on - strand, within Pf1N1B4_1228 at 1394.304 kb on - strand at 1394.304 kb on - strand, within Pf1N1B4_1228 at 1394.304 kb on - strand, within Pf1N1B4_1228 at 1395.051 kb on + strand at 1395.051 kb on + strand at 1395.193 kb on + strand, within Pf1N1B4_1229 at 1395.194 kb on - strand at 1395.327 kb on + strand, within Pf1N1B4_1229 at 1395.327 kb on + strand, within Pf1N1B4_1229 at 1395.327 kb on + strand, within Pf1N1B4_1229 at 1395.327 kb on + strand, within Pf1N1B4_1229 at 1395.327 kb on + strand, within Pf1N1B4_1229 at 1395.327 kb on + strand, within Pf1N1B4_1229 at 1395.327 kb on + strand, within Pf1N1B4_1229 at 1395.328 kb on - strand, within Pf1N1B4_1229 at 1395.328 kb on - strand, within Pf1N1B4_1229 at 1395.610 kb on + strand, within Pf1N1B4_1229 at 1395.736 kb on + strand at 1395.760 kb on - strand, within Pf1N1B4_1229 at 1395.760 kb on - strand, within Pf1N1B4_1229 at 1396.024 kb on + strand, within Pf1N1B4_1229 at 1396.059 kb on + strand, within Pf1N1B4_1229 at 1396.059 kb on + strand, within Pf1N1B4_1229 at 1396.059 kb on + strand, within Pf1N1B4_1229 at 1396.059 kb on + strand, within Pf1N1B4_1229 at 1396.059 kb on + strand, within Pf1N1B4_1229 at 1396.059 kb on + strand, within Pf1N1B4_1229 at 1396.059 kb on + strand, within Pf1N1B4_1229 at 1396.059 kb on + strand, within Pf1N1B4_1229 at 1396.060 kb on - strand, within Pf1N1B4_1229 at 1396.060 kb on - strand, within Pf1N1B4_1229 at 1396.060 kb on - strand, within Pf1N1B4_1229 at 1396.156 kb on + strand, within Pf1N1B4_1229 at 1396.202 kb on + strand, within Pf1N1B4_1229 at 1396.203 kb on - strand, within Pf1N1B4_1229 at 1396.214 kb on - strand, within Pf1N1B4_1229 at 1396.484 kb on - strand at 1396.506 kb on + strand at 1396.506 kb on + strand at 1396.506 kb on + strand at 1396.506 kb on + strand at 1396.506 kb on + strand at 1396.507 kb on - strand at 1396.507 kb on - strand at 1396.510 kb on + strand at 1396.510 kb on + strand at 1396.511 kb on - strand at 1396.511 kb on - strand at 1396.598 kb on + strand at 1396.598 kb on + strand at 1396.598 kb on + strand at 1396.598 kb on + strand at 1396.598 kb on + strand at 1396.598 kb on + strand at 1396.598 kb on + strand at 1396.598 kb on + strand at 1396.599 kb on - strand at 1396.599 kb on - strand at 1396.599 kb on - strand at 1396.599 kb on - strand at 1396.599 kb on - strand at 1396.599 kb on - strand at 1396.599 kb on - strand at 1396.599 kb on - strand at 1396.599 kb on - strand at 1396.599 kb on - strand at 1396.614 kb on + strand at 1396.615 kb on - strand at 1396.617 kb on + strand at 1396.629 kb on + strand at 1396.630 kb on - strand at 1396.667 kb on + strand at 1396.759 kb on + strand, within Pf1N1B4_1230 at 1396.759 kb on + strand, within Pf1N1B4_1230 at 1396.759 kb on + strand, within Pf1N1B4_1230 at 1396.760 kb on - strand, within Pf1N1B4_1230 at 1396.760 kb on - strand, within Pf1N1B4_1230 at 1396.760 kb on - strand, within Pf1N1B4_1230 at 1397.131 kb on + strand, within Pf1N1B4_1230 at 1397.131 kb on + strand, within Pf1N1B4_1230 at 1397.131 kb on + strand, within Pf1N1B4_1230 at 1397.132 kb on - strand, within Pf1N1B4_1230 at 1397.132 kb on - strand, within Pf1N1B4_1230 at 1397.170 kb on + strand, within Pf1N1B4_1230 at 1397.170 kb on + strand, within Pf1N1B4_1230 at 1397.170 kb on + strand, within Pf1N1B4_1230 at 1397.170 kb on + strand, within Pf1N1B4_1230 at 1397.171 kb on - strand, within Pf1N1B4_1230 at 1397.248 kb on + strand, within Pf1N1B4_1230 at 1397.248 kb on + strand, within Pf1N1B4_1230 at 1397.248 kb on + strand, within Pf1N1B4_1230 at 1397.248 kb on + strand, within Pf1N1B4_1230 at 1397.248 kb on + strand, within Pf1N1B4_1230 at 1397.249 kb on - strand, within Pf1N1B4_1230 at 1397.249 kb on - strand, within Pf1N1B4_1230 at 1397.249 kb on - strand, within Pf1N1B4_1230 at 1397.249 kb on - strand, within Pf1N1B4_1230 at 1397.284 kb on + strand, within Pf1N1B4_1230 at 1397.284 kb on + strand, within Pf1N1B4_1230 at 1397.285 kb on - strand, within Pf1N1B4_1230 at 1397.467 kb on + strand at 1397.468 kb on - strand, within Pf1N1B4_1230 at 1397.468 kb on - strand, within Pf1N1B4_1230 at 1397.468 kb on - strand, within Pf1N1B4_1230  
 
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction   LB             remove   1,393,978   -        +0.6   1,393,978   -        -1.8   1,394,144   +        +0.0   1,394,144   +        -0.4   1,394,144   +        -0.4   1,394,144   +        -0.4   1,394,145   -        +0.2   1,394,145   -        -0.8   1,394,303   +    Pf1N1B4_1228   0.26  +0.3   1,394,303   +    Pf1N1B4_1228   0.26  +0.6   1,394,303   +    Pf1N1B4_1228   0.26  -2.2   1,394,303   +        -0.4   1,394,303   +    Pf1N1B4_1228   0.26  +0.6   1,394,304   -    Pf1N1B4_1228   0.27  +0.2   1,394,304   -    Pf1N1B4_1228   0.27  +0.5   1,394,304   -    Pf1N1B4_1228   0.27  +0.1   1,394,304   -    Pf1N1B4_1228   0.27  -0.1   1,394,304   -    Pf1N1B4_1228   0.27  +0.6   1,394,304   -    Pf1N1B4_1228   0.27  -0.6   1,394,304   -        +0.8   1,394,304   -    Pf1N1B4_1228   0.27  +0.4   1,394,304   -    Pf1N1B4_1228   0.27  -1.1   1,395,051   +        -0.6   1,395,051   +        +0.9   1,395,193   +    Pf1N1B4_1229   0.15  +0.0   1,395,194   -        -0.8   1,395,327   +    Pf1N1B4_1229   0.24  +0.4   1,395,327   +    Pf1N1B4_1229   0.24  +0.4   1,395,327   +    Pf1N1B4_1229   0.24  +1.8   1,395,327   +    Pf1N1B4_1229   0.24  -2.4   1,395,327   +    Pf1N1B4_1229   0.24  -0.8   1,395,327   +    Pf1N1B4_1229   0.24  +0.3   1,395,327   +    Pf1N1B4_1229   0.24  -0.4   1,395,328   -    Pf1N1B4_1229   0.24  +0.9   1,395,328   -    Pf1N1B4_1229   0.24  -1.9   1,395,610   +    Pf1N1B4_1229   0.42  +0.5   1,395,736   +        -0.4   1,395,760   -    Pf1N1B4_1229   0.52  +1.6   1,395,760   -    Pf1N1B4_1229   0.52  +0.6   1,396,024   +    Pf1N1B4_1229   0.70  -0.5   1,396,059   +    Pf1N1B4_1229   0.72  +0.2   1,396,059   +    Pf1N1B4_1229   0.72  -0.1   1,396,059   +    Pf1N1B4_1229   0.72  -0.6   1,396,059   +    Pf1N1B4_1229   0.72  +2.1   1,396,059   +    Pf1N1B4_1229   0.72  -0.6   1,396,059   +    Pf1N1B4_1229   0.72  +0.6   1,396,059   +    Pf1N1B4_1229   0.72  -0.0   1,396,059   +    Pf1N1B4_1229   0.72  +0.4   1,396,060   -    Pf1N1B4_1229   0.72  +0.0   1,396,060   -    Pf1N1B4_1229   0.72  +0.9   1,396,060   -    Pf1N1B4_1229   0.72  +0.0   1,396,156   +    Pf1N1B4_1229   0.78  +2.6   1,396,202   +    Pf1N1B4_1229   0.81  +0.2   1,396,203   -    Pf1N1B4_1229   0.81  -0.1   1,396,214   -    Pf1N1B4_1229   0.82  +0.7   1,396,484   -        +0.7   1,396,506   +        +0.2   1,396,506   +        +1.6   1,396,506   +        -0.8   1,396,506   +        -0.5   1,396,506   +        +1.4   1,396,507   -        -0.2   1,396,507   -        +0.8   1,396,510   +        +0.2   1,396,510   +        -0.8   1,396,511   -        -0.4   1,396,511   -        +0.3   1,396,598   +        -1.1   1,396,598   +        +1.1   1,396,598   +        +1.4   1,396,598   +        +1.8   1,396,598   +        -0.3   1,396,598   +        -0.7   1,396,598   +        -1.5   1,396,598   +        -0.5   1,396,599   -        +0.4   1,396,599   -        -0.8   1,396,599   -        +1.0   1,396,599   -        -0.6   1,396,599   -        -0.1   1,396,599   -        -1.8   1,396,599   -        -0.1   1,396,599   -        -0.4   1,396,599   -        -1.1   1,396,599   -        +0.2   1,396,614   +        -0.2   1,396,615   -        -0.4   1,396,617   +        -0.3   1,396,629   +        -1.1   1,396,630   -        +0.9   1,396,667   +        -2.2   1,396,759   +    Pf1N1B4_1230   0.12  -2.1   1,396,759   +    Pf1N1B4_1230   0.12  -1.8   1,396,759   +    Pf1N1B4_1230   0.12  +0.6   1,396,760   -    Pf1N1B4_1230   0.12  -1.2   1,396,760   -    Pf1N1B4_1230   0.12  -1.8   1,396,760   -    Pf1N1B4_1230   0.12  +0.1   1,397,131   +    Pf1N1B4_1230   0.51  +0.2   1,397,131   +    Pf1N1B4_1230   0.51  +0.3   1,397,131   +    Pf1N1B4_1230   0.51  +0.0   1,397,132   -    Pf1N1B4_1230   0.51  +2.2   1,397,132   -    Pf1N1B4_1230   0.51  +0.5   1,397,170   +    Pf1N1B4_1230   0.55  +0.2   1,397,170   +    Pf1N1B4_1230   0.55  +1.2   1,397,170   +    Pf1N1B4_1230   0.55  -1.1   1,397,170   +    Pf1N1B4_1230   0.55  +0.8   1,397,171   -    Pf1N1B4_1230   0.55  +0.4   1,397,248   +    Pf1N1B4_1230   0.64  -2.5   1,397,248   +    Pf1N1B4_1230   0.64  +1.2   1,397,248   +    Pf1N1B4_1230   0.64  +0.5   1,397,248   +    Pf1N1B4_1230   0.64  +1.0   1,397,248   +    Pf1N1B4_1230   0.64  +0.4   1,397,249   -    Pf1N1B4_1230   0.64  -1.8   1,397,249   -    Pf1N1B4_1230   0.64  -2.2   1,397,249   -    Pf1N1B4_1230   0.64  +0.6   1,397,249   -    Pf1N1B4_1230   0.64  +0.6   1,397,284   +    Pf1N1B4_1230   0.67  -0.4   1,397,284   +    Pf1N1B4_1230   0.67  -0.4   1,397,285   -    Pf1N1B4_1230   0.68  +0.2   1,397,467   +        +1.8   1,397,468   -    Pf1N1B4_1230   0.87  -0.9   1,397,468   -    Pf1N1B4_1230   0.87  +0.2   1,397,468   -    Pf1N1B4_1230   0.87  +0.0 
 
Or see this region's nucleotide sequence