Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1228 and Pf1N1B4_1229 are separated by 10 nucleotides Pf1N1B4_1229 and Pf1N1B4_1230 are separated by 161 nucleotides
Pf1N1B4_1228 - 3-hydroxyisobutyrate dehydrogenase (EC 1.1.1.31), at 1,394,068 to 1,394,955
_1228
Pf1N1B4_1229 - Methylmalonate-semialdehyde dehydrogenase (EC 1.2.1.27) (from data) , at 1,394,966 to 1,396,483
_1229
Pf1N1B4_1230 - Transcriptional regulator, LysR family, at 1,396,645 to 1,397,592
_1230
Position (kb)
1394
1395
1396
1397 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1393.978 kb on - strand at 1393.978 kb on - strand at 1394.144 kb on + strand at 1394.144 kb on + strand at 1394.144 kb on + strand at 1394.144 kb on + strand at 1394.145 kb on - strand at 1394.145 kb on - strand at 1394.303 kb on + strand, within Pf1N1B4_1228 at 1394.303 kb on + strand, within Pf1N1B4_1228 at 1394.303 kb on + strand, within Pf1N1B4_1228 at 1394.303 kb on + strand at 1394.303 kb on + strand, within Pf1N1B4_1228 at 1394.304 kb on - strand, within Pf1N1B4_1228 at 1394.304 kb on - strand, within Pf1N1B4_1228 at 1394.304 kb on - strand, within Pf1N1B4_1228 at 1394.304 kb on - strand, within Pf1N1B4_1228 at 1394.304 kb on - strand, within Pf1N1B4_1228 at 1394.304 kb on - strand, within Pf1N1B4_1228 at 1394.304 kb on - strand at 1394.304 kb on - strand, within Pf1N1B4_1228 at 1394.304 kb on - strand, within Pf1N1B4_1228 at 1395.051 kb on + strand at 1395.051 kb on + strand at 1395.193 kb on + strand, within Pf1N1B4_1229 at 1395.194 kb on - strand at 1395.327 kb on + strand, within Pf1N1B4_1229 at 1395.327 kb on + strand, within Pf1N1B4_1229 at 1395.327 kb on + strand, within Pf1N1B4_1229 at 1395.327 kb on + strand, within Pf1N1B4_1229 at 1395.327 kb on + strand, within Pf1N1B4_1229 at 1395.327 kb on + strand, within Pf1N1B4_1229 at 1395.327 kb on + strand, within Pf1N1B4_1229 at 1395.328 kb on - strand, within Pf1N1B4_1229 at 1395.328 kb on - strand, within Pf1N1B4_1229 at 1395.610 kb on + strand, within Pf1N1B4_1229 at 1395.736 kb on + strand at 1395.760 kb on - strand, within Pf1N1B4_1229 at 1395.760 kb on - strand, within Pf1N1B4_1229 at 1396.024 kb on + strand, within Pf1N1B4_1229 at 1396.059 kb on + strand, within Pf1N1B4_1229 at 1396.059 kb on + strand, within Pf1N1B4_1229 at 1396.059 kb on + strand, within Pf1N1B4_1229 at 1396.059 kb on + strand, within Pf1N1B4_1229 at 1396.059 kb on + strand, within Pf1N1B4_1229 at 1396.059 kb on + strand, within Pf1N1B4_1229 at 1396.059 kb on + strand, within Pf1N1B4_1229 at 1396.059 kb on + strand, within Pf1N1B4_1229 at 1396.060 kb on - strand, within Pf1N1B4_1229 at 1396.060 kb on - strand, within Pf1N1B4_1229 at 1396.060 kb on - strand, within Pf1N1B4_1229 at 1396.156 kb on + strand, within Pf1N1B4_1229 at 1396.202 kb on + strand, within Pf1N1B4_1229 at 1396.203 kb on - strand, within Pf1N1B4_1229 at 1396.214 kb on - strand, within Pf1N1B4_1229 at 1396.484 kb on - strand at 1396.506 kb on + strand at 1396.506 kb on + strand at 1396.506 kb on + strand at 1396.506 kb on + strand at 1396.506 kb on + strand at 1396.507 kb on - strand at 1396.507 kb on - strand at 1396.510 kb on + strand at 1396.510 kb on + strand at 1396.511 kb on - strand at 1396.511 kb on - strand at 1396.598 kb on + strand at 1396.598 kb on + strand at 1396.598 kb on + strand at 1396.598 kb on + strand at 1396.598 kb on + strand at 1396.598 kb on + strand at 1396.598 kb on + strand at 1396.598 kb on + strand at 1396.599 kb on - strand at 1396.599 kb on - strand at 1396.599 kb on - strand at 1396.599 kb on - strand at 1396.599 kb on - strand at 1396.599 kb on - strand at 1396.599 kb on - strand at 1396.599 kb on - strand at 1396.599 kb on - strand at 1396.599 kb on - strand at 1396.614 kb on + strand at 1396.615 kb on - strand at 1396.617 kb on + strand at 1396.629 kb on + strand at 1396.630 kb on - strand at 1396.667 kb on + strand at 1396.759 kb on + strand, within Pf1N1B4_1230 at 1396.759 kb on + strand, within Pf1N1B4_1230 at 1396.759 kb on + strand, within Pf1N1B4_1230 at 1396.760 kb on - strand, within Pf1N1B4_1230 at 1396.760 kb on - strand, within Pf1N1B4_1230 at 1396.760 kb on - strand, within Pf1N1B4_1230 at 1397.131 kb on + strand, within Pf1N1B4_1230 at 1397.131 kb on + strand, within Pf1N1B4_1230 at 1397.131 kb on + strand, within Pf1N1B4_1230 at 1397.132 kb on - strand, within Pf1N1B4_1230 at 1397.132 kb on - strand, within Pf1N1B4_1230 at 1397.170 kb on + strand, within Pf1N1B4_1230 at 1397.170 kb on + strand, within Pf1N1B4_1230 at 1397.170 kb on + strand, within Pf1N1B4_1230 at 1397.170 kb on + strand, within Pf1N1B4_1230 at 1397.171 kb on - strand, within Pf1N1B4_1230 at 1397.248 kb on + strand, within Pf1N1B4_1230 at 1397.248 kb on + strand, within Pf1N1B4_1230 at 1397.248 kb on + strand, within Pf1N1B4_1230 at 1397.248 kb on + strand, within Pf1N1B4_1230 at 1397.248 kb on + strand, within Pf1N1B4_1230 at 1397.249 kb on - strand, within Pf1N1B4_1230 at 1397.249 kb on - strand, within Pf1N1B4_1230 at 1397.249 kb on - strand, within Pf1N1B4_1230 at 1397.249 kb on - strand, within Pf1N1B4_1230 at 1397.284 kb on + strand, within Pf1N1B4_1230 at 1397.284 kb on + strand, within Pf1N1B4_1230 at 1397.285 kb on - strand, within Pf1N1B4_1230 at 1397.467 kb on + strand at 1397.468 kb on - strand, within Pf1N1B4_1230 at 1397.468 kb on - strand, within Pf1N1B4_1230 at 1397.468 kb on - strand, within Pf1N1B4_1230
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,393,978 - +0.6 1,393,978 - -1.8 1,394,144 + +0.0 1,394,144 + -0.4 1,394,144 + -0.4 1,394,144 + -0.4 1,394,145 - +0.2 1,394,145 - -0.8 1,394,303 + Pf1N1B4_1228 0.26 +0.3 1,394,303 + Pf1N1B4_1228 0.26 +0.6 1,394,303 + Pf1N1B4_1228 0.26 -2.2 1,394,303 + -0.4 1,394,303 + Pf1N1B4_1228 0.26 +0.6 1,394,304 - Pf1N1B4_1228 0.27 +0.2 1,394,304 - Pf1N1B4_1228 0.27 +0.5 1,394,304 - Pf1N1B4_1228 0.27 +0.1 1,394,304 - Pf1N1B4_1228 0.27 -0.1 1,394,304 - Pf1N1B4_1228 0.27 +0.6 1,394,304 - Pf1N1B4_1228 0.27 -0.6 1,394,304 - +0.8 1,394,304 - Pf1N1B4_1228 0.27 +0.4 1,394,304 - Pf1N1B4_1228 0.27 -1.1 1,395,051 + -0.6 1,395,051 + +0.9 1,395,193 + Pf1N1B4_1229 0.15 +0.0 1,395,194 - -0.8 1,395,327 + Pf1N1B4_1229 0.24 +0.4 1,395,327 + Pf1N1B4_1229 0.24 +0.4 1,395,327 + Pf1N1B4_1229 0.24 +1.8 1,395,327 + Pf1N1B4_1229 0.24 -2.4 1,395,327 + Pf1N1B4_1229 0.24 -0.8 1,395,327 + Pf1N1B4_1229 0.24 +0.3 1,395,327 + Pf1N1B4_1229 0.24 -0.4 1,395,328 - Pf1N1B4_1229 0.24 +0.9 1,395,328 - Pf1N1B4_1229 0.24 -1.9 1,395,610 + Pf1N1B4_1229 0.42 +0.5 1,395,736 + -0.4 1,395,760 - Pf1N1B4_1229 0.52 +1.6 1,395,760 - Pf1N1B4_1229 0.52 +0.6 1,396,024 + Pf1N1B4_1229 0.70 -0.5 1,396,059 + Pf1N1B4_1229 0.72 +0.2 1,396,059 + Pf1N1B4_1229 0.72 -0.1 1,396,059 + Pf1N1B4_1229 0.72 -0.6 1,396,059 + Pf1N1B4_1229 0.72 +2.1 1,396,059 + Pf1N1B4_1229 0.72 -0.6 1,396,059 + Pf1N1B4_1229 0.72 +0.6 1,396,059 + Pf1N1B4_1229 0.72 -0.0 1,396,059 + Pf1N1B4_1229 0.72 +0.4 1,396,060 - Pf1N1B4_1229 0.72 +0.0 1,396,060 - Pf1N1B4_1229 0.72 +0.9 1,396,060 - Pf1N1B4_1229 0.72 +0.0 1,396,156 + Pf1N1B4_1229 0.78 +2.6 1,396,202 + Pf1N1B4_1229 0.81 +0.2 1,396,203 - Pf1N1B4_1229 0.81 -0.1 1,396,214 - Pf1N1B4_1229 0.82 +0.7 1,396,484 - +0.7 1,396,506 + +0.2 1,396,506 + +1.6 1,396,506 + -0.8 1,396,506 + -0.5 1,396,506 + +1.4 1,396,507 - -0.2 1,396,507 - +0.8 1,396,510 + +0.2 1,396,510 + -0.8 1,396,511 - -0.4 1,396,511 - +0.3 1,396,598 + -1.1 1,396,598 + +1.1 1,396,598 + +1.4 1,396,598 + +1.8 1,396,598 + -0.3 1,396,598 + -0.7 1,396,598 + -1.5 1,396,598 + -0.5 1,396,599 - +0.4 1,396,599 - -0.8 1,396,599 - +1.0 1,396,599 - -0.6 1,396,599 - -0.1 1,396,599 - -1.8 1,396,599 - -0.1 1,396,599 - -0.4 1,396,599 - -1.1 1,396,599 - +0.2 1,396,614 + -0.2 1,396,615 - -0.4 1,396,617 + -0.3 1,396,629 + -1.1 1,396,630 - +0.9 1,396,667 + -2.2 1,396,759 + Pf1N1B4_1230 0.12 -2.1 1,396,759 + Pf1N1B4_1230 0.12 -1.8 1,396,759 + Pf1N1B4_1230 0.12 +0.6 1,396,760 - Pf1N1B4_1230 0.12 -1.2 1,396,760 - Pf1N1B4_1230 0.12 -1.8 1,396,760 - Pf1N1B4_1230 0.12 +0.1 1,397,131 + Pf1N1B4_1230 0.51 +0.2 1,397,131 + Pf1N1B4_1230 0.51 +0.3 1,397,131 + Pf1N1B4_1230 0.51 +0.0 1,397,132 - Pf1N1B4_1230 0.51 +2.2 1,397,132 - Pf1N1B4_1230 0.51 +0.5 1,397,170 + Pf1N1B4_1230 0.55 +0.2 1,397,170 + Pf1N1B4_1230 0.55 +1.2 1,397,170 + Pf1N1B4_1230 0.55 -1.1 1,397,170 + Pf1N1B4_1230 0.55 +0.8 1,397,171 - Pf1N1B4_1230 0.55 +0.4 1,397,248 + Pf1N1B4_1230 0.64 -2.5 1,397,248 + Pf1N1B4_1230 0.64 +1.2 1,397,248 + Pf1N1B4_1230 0.64 +0.5 1,397,248 + Pf1N1B4_1230 0.64 +1.0 1,397,248 + Pf1N1B4_1230 0.64 +0.4 1,397,249 - Pf1N1B4_1230 0.64 -1.8 1,397,249 - Pf1N1B4_1230 0.64 -2.2 1,397,249 - Pf1N1B4_1230 0.64 +0.6 1,397,249 - Pf1N1B4_1230 0.64 +0.6 1,397,284 + Pf1N1B4_1230 0.67 -0.4 1,397,284 + Pf1N1B4_1230 0.67 -0.4 1,397,285 - Pf1N1B4_1230 0.68 +0.2 1,397,467 + +1.8 1,397,468 - Pf1N1B4_1230 0.87 -0.9 1,397,468 - Pf1N1B4_1230 0.87 +0.2 1,397,468 - Pf1N1B4_1230 0.87 +0.0
Or see this region's nucleotide sequence