Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1211 and Pf1N1B4_1212 are separated by 418 nucleotides Pf1N1B4_1212 and Pf1N1B4_1213 are separated by 84 nucleotides Pf1N1B4_1213 and Pf1N1B4_1214 are separated by 138 nucleotides
Pf1N1B4_1211 - Hydrogen peroxide-inducible genes activator, at 1,373,726 to 1,374,658
_1211
Pf1N1B4_1212 - Predicted flavin reductase RutF in novel pyrimidine catabolism pathway, at 1,375,077 to 1,375,379
_1212
Pf1N1B4_1213 - Transcriptional regulator, at 1,375,464 to 1,375,739
_1213
Pf1N1B4_1214 - Chorismate--pyruvate lyase (EC 4.1.3.40), at 1,375,878 to 1,376,408
_1214
Position (kb)
1375
1376 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1374.273 kb on + strand, within Pf1N1B4_1211 at 1374.273 kb on + strand, within Pf1N1B4_1211 at 1374.302 kb on + strand, within Pf1N1B4_1211 at 1374.302 kb on + strand, within Pf1N1B4_1211 at 1374.303 kb on - strand at 1374.303 kb on - strand, within Pf1N1B4_1211 at 1374.303 kb on - strand, within Pf1N1B4_1211 at 1374.303 kb on - strand, within Pf1N1B4_1211 at 1374.303 kb on - strand, within Pf1N1B4_1211 at 1374.303 kb on - strand, within Pf1N1B4_1211 at 1374.303 kb on - strand, within Pf1N1B4_1211 at 1374.303 kb on - strand, within Pf1N1B4_1211 at 1374.303 kb on - strand at 1374.303 kb on - strand, within Pf1N1B4_1211 at 1374.338 kb on + strand, within Pf1N1B4_1211 at 1374.338 kb on + strand, within Pf1N1B4_1211 at 1374.651 kb on + strand at 1374.651 kb on + strand at 1374.651 kb on + strand at 1374.652 kb on - strand at 1374.652 kb on - strand at 1374.652 kb on - strand at 1374.652 kb on - strand at 1374.724 kb on + strand at 1374.724 kb on + strand at 1374.725 kb on - strand at 1374.731 kb on - strand at 1374.737 kb on + strand at 1374.737 kb on + strand at 1374.738 kb on - strand at 1374.760 kb on - strand at 1374.760 kb on - strand at 1374.760 kb on - strand at 1374.760 kb on - strand at 1374.760 kb on - strand at 1374.763 kb on + strand at 1374.763 kb on + strand at 1374.763 kb on + strand at 1374.763 kb on + strand at 1374.764 kb on - strand at 1374.764 kb on - strand at 1374.764 kb on - strand at 1374.764 kb on - strand at 1374.766 kb on + strand at 1374.767 kb on - strand at 1374.768 kb on + strand at 1374.847 kb on + strand at 1374.852 kb on + strand at 1374.852 kb on + strand at 1374.852 kb on + strand at 1374.852 kb on + strand at 1374.852 kb on + strand at 1374.853 kb on - strand at 1374.853 kb on - strand at 1374.853 kb on - strand at 1374.853 kb on - strand at 1374.853 kb on - strand at 1374.853 kb on - strand at 1374.853 kb on - strand at 1374.853 kb on - strand at 1374.853 kb on - strand at 1374.872 kb on + strand at 1374.872 kb on + strand at 1374.873 kb on - strand at 1374.873 kb on - strand at 1374.897 kb on - strand at 1375.320 kb on + strand, within Pf1N1B4_1212 at 1375.321 kb on - strand, within Pf1N1B4_1212 at 1375.335 kb on + strand, within Pf1N1B4_1212 at 1375.335 kb on + strand, within Pf1N1B4_1212 at 1375.335 kb on + strand, within Pf1N1B4_1212 at 1375.335 kb on + strand, within Pf1N1B4_1212 at 1375.335 kb on + strand, within Pf1N1B4_1212 at 1375.335 kb on + strand, within Pf1N1B4_1212 at 1375.336 kb on - strand, within Pf1N1B4_1212 at 1375.336 kb on - strand, within Pf1N1B4_1212 at 1375.469 kb on - strand at 1375.494 kb on - strand, within Pf1N1B4_1213 at 1375.494 kb on - strand, within Pf1N1B4_1213 at 1375.495 kb on + strand, within Pf1N1B4_1213 at 1375.496 kb on - strand, within Pf1N1B4_1213 at 1375.496 kb on - strand, within Pf1N1B4_1213 at 1375.502 kb on + strand, within Pf1N1B4_1213 at 1375.502 kb on + strand, within Pf1N1B4_1213 at 1375.503 kb on - strand, within Pf1N1B4_1213 at 1375.503 kb on - strand, within Pf1N1B4_1213 at 1375.503 kb on - strand, within Pf1N1B4_1213 at 1375.503 kb on - strand, within Pf1N1B4_1213 at 1375.503 kb on - strand, within Pf1N1B4_1213 at 1375.514 kb on + strand, within Pf1N1B4_1213 at 1375.515 kb on - strand, within Pf1N1B4_1213 at 1375.515 kb on - strand, within Pf1N1B4_1213 at 1375.515 kb on - strand, within Pf1N1B4_1213 at 1375.515 kb on - strand, within Pf1N1B4_1213 at 1375.547 kb on + strand, within Pf1N1B4_1213 at 1375.717 kb on + strand at 1375.717 kb on + strand at 1375.718 kb on - strand at 1375.718 kb on - strand at 1375.817 kb on - strand at 1375.817 kb on - strand at 1375.817 kb on - strand at 1375.826 kb on + strand at 1375.994 kb on - strand, within Pf1N1B4_1214 at 1376.069 kb on + strand, within Pf1N1B4_1214 at 1376.069 kb on + strand, within Pf1N1B4_1214 at 1376.069 kb on + strand, within Pf1N1B4_1214 at 1376.069 kb on + strand, within Pf1N1B4_1214 at 1376.069 kb on + strand, within Pf1N1B4_1214 at 1376.069 kb on + strand, within Pf1N1B4_1214 at 1376.069 kb on + strand, within Pf1N1B4_1214 at 1376.069 kb on + strand, within Pf1N1B4_1214 at 1376.070 kb on - strand, within Pf1N1B4_1214 at 1376.070 kb on - strand, within Pf1N1B4_1214 at 1376.070 kb on - strand, within Pf1N1B4_1214 at 1376.070 kb on - strand, within Pf1N1B4_1214 at 1376.070 kb on - strand, within Pf1N1B4_1214 at 1376.070 kb on - strand, within Pf1N1B4_1214 at 1376.070 kb on - strand, within Pf1N1B4_1214 at 1376.070 kb on - strand, within Pf1N1B4_1214 at 1376.101 kb on + strand, within Pf1N1B4_1214 at 1376.293 kb on + strand, within Pf1N1B4_1214 at 1376.293 kb on + strand, within Pf1N1B4_1214 at 1376.293 kb on + strand, within Pf1N1B4_1214 at 1376.364 kb on + strand at 1376.365 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,374,273 + Pf1N1B4_1211 0.59 -1.4 1,374,273 + Pf1N1B4_1211 0.59 -0.4 1,374,302 + Pf1N1B4_1211 0.62 -0.4 1,374,302 + Pf1N1B4_1211 0.62 -0.2 1,374,303 - +1.2 1,374,303 - Pf1N1B4_1211 0.62 +1.2 1,374,303 - Pf1N1B4_1211 0.62 -1.8 1,374,303 - Pf1N1B4_1211 0.62 +0.6 1,374,303 - Pf1N1B4_1211 0.62 -0.3 1,374,303 - Pf1N1B4_1211 0.62 -1.1 1,374,303 - Pf1N1B4_1211 0.62 -0.2 1,374,303 - Pf1N1B4_1211 0.62 -0.9 1,374,303 - +1.2 1,374,303 - Pf1N1B4_1211 0.62 -2.2 1,374,338 + Pf1N1B4_1211 0.66 +0.4 1,374,338 + Pf1N1B4_1211 0.66 -2.0 1,374,651 + +0.7 1,374,651 + +0.6 1,374,651 + -0.8 1,374,652 - +0.4 1,374,652 - -0.4 1,374,652 - -0.8 1,374,652 - -0.2 1,374,724 + +0.6 1,374,724 + -0.4 1,374,725 - -2.4 1,374,731 - +1.2 1,374,737 + +0.5 1,374,737 + -1.8 1,374,738 - -0.4 1,374,760 - -2.1 1,374,760 - -0.4 1,374,760 - -0.4 1,374,760 - +0.6 1,374,760 - +0.9 1,374,763 + +0.5 1,374,763 + +1.0 1,374,763 + +0.2 1,374,763 + +0.2 1,374,764 - -0.6 1,374,764 - -0.0 1,374,764 - -0.6 1,374,764 - -2.1 1,374,766 + +0.1 1,374,767 - +0.2 1,374,768 + +0.7 1,374,847 + +1.2 1,374,852 + -0.1 1,374,852 + +0.1 1,374,852 + -1.5 1,374,852 + +1.2 1,374,852 + +1.9 1,374,853 - -1.4 1,374,853 - -0.4 1,374,853 - -1.0 1,374,853 - -0.1 1,374,853 - +1.2 1,374,853 - +0.1 1,374,853 - -1.0 1,374,853 - +1.2 1,374,853 - -0.0 1,374,872 + -0.1 1,374,872 + -1.4 1,374,873 - -0.8 1,374,873 - -1.1 1,374,897 - -0.2 1,375,320 + Pf1N1B4_1212 0.80 -1.7 1,375,321 - Pf1N1B4_1212 0.81 -1.4 1,375,335 + Pf1N1B4_1212 0.85 -0.2 1,375,335 + Pf1N1B4_1212 0.85 -0.2 1,375,335 + Pf1N1B4_1212 0.85 -0.4 1,375,335 + Pf1N1B4_1212 0.85 -0.5 1,375,335 + Pf1N1B4_1212 0.85 -1.6 1,375,335 + Pf1N1B4_1212 0.85 -1.0 1,375,336 - Pf1N1B4_1212 0.85 -1.4 1,375,336 - Pf1N1B4_1212 0.85 +0.6 1,375,469 - -1.0 1,375,494 - Pf1N1B4_1213 0.11 +0.4 1,375,494 - Pf1N1B4_1213 0.11 +0.5 1,375,495 + Pf1N1B4_1213 0.11 -1.4 1,375,496 - Pf1N1B4_1213 0.12 +0.6 1,375,496 - Pf1N1B4_1213 0.12 +0.1 1,375,502 + Pf1N1B4_1213 0.14 +0.6 1,375,502 + Pf1N1B4_1213 0.14 +1.2 1,375,503 - Pf1N1B4_1213 0.14 -1.8 1,375,503 - Pf1N1B4_1213 0.14 -0.6 1,375,503 - Pf1N1B4_1213 0.14 -1.2 1,375,503 - Pf1N1B4_1213 0.14 +0.2 1,375,503 - Pf1N1B4_1213 0.14 +0.9 1,375,514 + Pf1N1B4_1213 0.18 -2.2 1,375,515 - Pf1N1B4_1213 0.18 -1.5 1,375,515 - Pf1N1B4_1213 0.18 +0.2 1,375,515 - Pf1N1B4_1213 0.18 -0.3 1,375,515 - Pf1N1B4_1213 0.18 -1.4 1,375,547 + Pf1N1B4_1213 0.30 -0.7 1,375,717 + +0.6 1,375,717 + -0.4 1,375,718 - -1.4 1,375,718 - +0.2 1,375,817 - +0.9 1,375,817 - -0.1 1,375,817 - +0.1 1,375,826 + +0.0 1,375,994 - Pf1N1B4_1214 0.22 +0.6 1,376,069 + Pf1N1B4_1214 0.36 -1.0 1,376,069 + Pf1N1B4_1214 0.36 +0.6 1,376,069 + Pf1N1B4_1214 0.36 -1.4 1,376,069 + Pf1N1B4_1214 0.36 +1.0 1,376,069 + Pf1N1B4_1214 0.36 -0.5 1,376,069 + Pf1N1B4_1214 0.36 -0.7 1,376,069 + Pf1N1B4_1214 0.36 +0.8 1,376,069 + Pf1N1B4_1214 0.36 +0.3 1,376,070 - Pf1N1B4_1214 0.36 -1.4 1,376,070 - Pf1N1B4_1214 0.36 -0.4 1,376,070 - Pf1N1B4_1214 0.36 -1.6 1,376,070 - Pf1N1B4_1214 0.36 -0.6 1,376,070 - Pf1N1B4_1214 0.36 -1.8 1,376,070 - Pf1N1B4_1214 0.36 +0.4 1,376,070 - Pf1N1B4_1214 0.36 -0.6 1,376,070 - Pf1N1B4_1214 0.36 -0.5 1,376,101 + Pf1N1B4_1214 0.42 -0.4 1,376,293 + Pf1N1B4_1214 0.78 -0.8 1,376,293 + Pf1N1B4_1214 0.78 -1.1 1,376,293 + Pf1N1B4_1214 0.78 -1.1 1,376,364 + +0.3 1,376,365 - +0.8
Or see this region's nucleotide sequence