Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_105 and Pf1N1B4_106 are separated by 3 nucleotides Pf1N1B4_106 and Pf1N1B4_107 are separated by 38 nucleotides Pf1N1B4_107 and Pf1N1B4_108 are separated by 243 nucleotides Pf1N1B4_108 and Pf1N1B4_109 are separated by 82 nucleotides Pf1N1B4_109 and Pf1N1B4_110 are separated by 17 nucleotides Pf1N1B4_110 and Pf1N1B4_111 are separated by 226 nucleotides
Pf1N1B4_105 - Two-component system response regulator QseB, at 111,675 to 112,343
_105
Pf1N1B4_106 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 112,347 to 112,628
_106
Pf1N1B4_107 - FIG00857858: hypothetical protein, at 112,667 to 112,975
_107
Pf1N1B4_108 - FIG00954456: hypothetical protein, at 113,219 to 114,298
_108
Pf1N1B4_109 - hypothetical protein, at 114,381 to 114,536
_109
Pf1N1B4_110 - Queuosine biosynthesis QueD, PTPS-I, at 114,554 to 114,910
_110
Pf1N1B4_111 - Possible oxidoreductase, at 115,137 to 115,961
_111
Position (kb)
113
114
115 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 112.232 kb on - strand, within Pf1N1B4_105 at 112.364 kb on + strand at 112.364 kb on + strand at 112.368 kb on + strand at 112.368 kb on + strand at 112.368 kb on + strand at 112.369 kb on - strand at 112.395 kb on + strand, within Pf1N1B4_106 at 112.409 kb on + strand, within Pf1N1B4_106 at 112.438 kb on + strand, within Pf1N1B4_106 at 112.438 kb on + strand at 112.438 kb on + strand, within Pf1N1B4_106 at 112.444 kb on + strand, within Pf1N1B4_106 at 112.668 kb on + strand at 112.668 kb on + strand at 112.668 kb on + strand at 112.668 kb on + strand at 112.668 kb on + strand at 112.761 kb on + strand, within Pf1N1B4_107 at 112.762 kb on - strand, within Pf1N1B4_107 at 112.767 kb on + strand, within Pf1N1B4_107 at 112.767 kb on + strand, within Pf1N1B4_107 at 112.767 kb on + strand, within Pf1N1B4_107 at 112.768 kb on - strand, within Pf1N1B4_107 at 112.768 kb on - strand, within Pf1N1B4_107 at 112.768 kb on - strand, within Pf1N1B4_107 at 112.768 kb on - strand, within Pf1N1B4_107 at 112.777 kb on - strand at 112.864 kb on + strand, within Pf1N1B4_107 at 112.865 kb on - strand, within Pf1N1B4_107 at 112.988 kb on + strand at 113.006 kb on + strand at 113.006 kb on + strand at 113.007 kb on - strand at 113.007 kb on - strand at 113.016 kb on - strand at 113.016 kb on - strand at 113.016 kb on - strand at 113.020 kb on + strand at 113.020 kb on + strand at 113.035 kb on - strand at 113.087 kb on - strand at 113.126 kb on + strand at 113.127 kb on - strand at 113.128 kb on + strand at 113.154 kb on + strand at 113.154 kb on + strand at 113.155 kb on - strand at 113.510 kb on + strand, within Pf1N1B4_108 at 113.510 kb on + strand, within Pf1N1B4_108 at 113.961 kb on - strand, within Pf1N1B4_108 at 113.979 kb on - strand, within Pf1N1B4_108 at 114.116 kb on + strand, within Pf1N1B4_108 at 114.116 kb on + strand, within Pf1N1B4_108 at 114.116 kb on + strand, within Pf1N1B4_108 at 114.116 kb on + strand, within Pf1N1B4_108 at 114.200 kb on + strand at 114.201 kb on - strand at 114.234 kb on + strand at 114.315 kb on + strand at 114.316 kb on - strand at 114.494 kb on - strand, within Pf1N1B4_109 at 114.740 kb on + strand, within Pf1N1B4_110 at 114.740 kb on + strand, within Pf1N1B4_110 at 114.741 kb on - strand, within Pf1N1B4_110 at 114.757 kb on + strand, within Pf1N1B4_110 at 114.758 kb on - strand at 114.758 kb on - strand, within Pf1N1B4_110 at 114.758 kb on - strand, within Pf1N1B4_110 at 114.758 kb on - strand, within Pf1N1B4_110 at 114.758 kb on - strand, within Pf1N1B4_110 at 114.761 kb on + strand, within Pf1N1B4_110 at 114.761 kb on + strand, within Pf1N1B4_110 at 114.762 kb on - strand, within Pf1N1B4_110 at 114.762 kb on - strand, within Pf1N1B4_110 at 114.762 kb on - strand at 114.896 kb on + strand at 115.064 kb on - strand at 115.099 kb on + strand at 115.099 kb on + strand at 115.099 kb on + strand at 115.099 kb on + strand at 115.099 kb on + strand at 115.099 kb on + strand at 115.100 kb on - strand at 115.100 kb on - strand at 115.100 kb on - strand at 115.165 kb on - strand at 115.198 kb on + strand at 115.251 kb on + strand, within Pf1N1B4_111 at 115.251 kb on + strand, within Pf1N1B4_111 at 115.251 kb on + strand, within Pf1N1B4_111 at 115.251 kb on + strand, within Pf1N1B4_111 at 115.252 kb on - strand, within Pf1N1B4_111 at 115.252 kb on - strand, within Pf1N1B4_111 at 115.252 kb on - strand, within Pf1N1B4_111 at 115.255 kb on + strand, within Pf1N1B4_111 at 115.255 kb on + strand at 115.255 kb on + strand, within Pf1N1B4_111 at 115.255 kb on + strand, within Pf1N1B4_111 at 115.256 kb on - strand, within Pf1N1B4_111 at 115.256 kb on - strand, within Pf1N1B4_111 at 115.256 kb on - strand, within Pf1N1B4_111 at 115.256 kb on - strand, within Pf1N1B4_111 at 115.256 kb on - strand, within Pf1N1B4_111
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 112,232 - Pf1N1B4_105 0.83 -0.6 112,364 + -2.0 112,364 + +0.1 112,368 + -0.0 112,368 + +0.1 112,368 + +0.3 112,369 - -1.8 112,395 + Pf1N1B4_106 0.17 -0.3 112,409 + Pf1N1B4_106 0.22 +3.4 112,438 + Pf1N1B4_106 0.32 -0.4 112,438 + -0.2 112,438 + Pf1N1B4_106 0.32 -1.1 112,444 + Pf1N1B4_106 0.34 -0.6 112,668 + +2.8 112,668 + +0.3 112,668 + -1.0 112,668 + -0.8 112,668 + +0.5 112,761 + Pf1N1B4_107 0.30 -1.2 112,762 - Pf1N1B4_107 0.31 +1.4 112,767 + Pf1N1B4_107 0.32 +0.4 112,767 + Pf1N1B4_107 0.32 +0.8 112,767 + Pf1N1B4_107 0.32 +0.2 112,768 - Pf1N1B4_107 0.33 -0.5 112,768 - Pf1N1B4_107 0.33 -1.6 112,768 - Pf1N1B4_107 0.33 +0.1 112,768 - Pf1N1B4_107 0.33 +2.8 112,777 - +0.8 112,864 + Pf1N1B4_107 0.64 -1.0 112,865 - Pf1N1B4_107 0.64 -0.2 112,988 + +1.4 113,006 + +0.8 113,006 + -1.6 113,007 - -1.2 113,007 - -0.6 113,016 - -0.4 113,016 - +0.4 113,016 - +0.4 113,020 + +0.5 113,020 + +0.5 113,035 - -0.6 113,087 - +0.2 113,126 + -0.2 113,127 - +0.2 113,128 + +0.1 113,154 + +0.0 113,154 + -0.3 113,155 - +2.1 113,510 + Pf1N1B4_108 0.27 +1.1 113,510 + Pf1N1B4_108 0.27 +0.2 113,961 - Pf1N1B4_108 0.69 +0.8 113,979 - Pf1N1B4_108 0.70 +0.8 114,116 + Pf1N1B4_108 0.83 +0.0 114,116 + Pf1N1B4_108 0.83 -0.8 114,116 + Pf1N1B4_108 0.83 +0.8 114,116 + Pf1N1B4_108 0.83 +0.3 114,200 + +0.4 114,201 - -1.6 114,234 + -0.7 114,315 + -1.0 114,316 - +0.5 114,494 - Pf1N1B4_109 0.72 -0.4 114,740 + Pf1N1B4_110 0.52 +0.1 114,740 + Pf1N1B4_110 0.52 -0.6 114,741 - Pf1N1B4_110 0.52 +1.1 114,757 + Pf1N1B4_110 0.57 -0.2 114,758 - +1.8 114,758 - Pf1N1B4_110 0.57 -0.2 114,758 - Pf1N1B4_110 0.57 +0.0 114,758 - Pf1N1B4_110 0.57 -0.2 114,758 - Pf1N1B4_110 0.57 +0.8 114,761 + Pf1N1B4_110 0.58 +0.0 114,761 + Pf1N1B4_110 0.58 -0.1 114,762 - Pf1N1B4_110 0.58 -1.2 114,762 - Pf1N1B4_110 0.58 +0.8 114,762 - +0.4 114,896 + +1.1 115,064 - +0.9 115,099 + +0.2 115,099 + -0.8 115,099 + -0.2 115,099 + +0.3 115,099 + -1.8 115,099 + -1.2 115,100 - +2.0 115,100 - -0.2 115,100 - +0.5 115,165 - -0.2 115,198 + +1.4 115,251 + Pf1N1B4_111 0.14 -0.2 115,251 + Pf1N1B4_111 0.14 -0.8 115,251 + Pf1N1B4_111 0.14 -1.0 115,251 + Pf1N1B4_111 0.14 +0.4 115,252 - Pf1N1B4_111 0.14 -0.1 115,252 - Pf1N1B4_111 0.14 +0.8 115,252 - Pf1N1B4_111 0.14 +0.2 115,255 + Pf1N1B4_111 0.14 +0.0 115,255 + +1.4 115,255 + Pf1N1B4_111 0.14 -1.2 115,255 + Pf1N1B4_111 0.14 -0.9 115,256 - Pf1N1B4_111 0.14 -1.3 115,256 - Pf1N1B4_111 0.14 +1.9 115,256 - Pf1N1B4_111 0.14 +0.0 115,256 - Pf1N1B4_111 0.14 +0.3 115,256 - Pf1N1B4_111 0.14 +0.5
Or see this region's nucleotide sequence