Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1002 and Pf1N1B4_1003 are separated by 373 nucleotides Pf1N1B4_1003 and Pf1N1B4_1004 are separated by 239 nucleotides Pf1N1B4_1004 and Pf1N1B4_1005 are separated by 336 nucleotides Pf1N1B4_1005 and Pf1N1B4_1006 are separated by 21 nucleotides
Pf1N1B4_1002 - LysR family transcriptional regulator QseA, at 1,137,186 to 1,138,154
_1002
Pf1N1B4_1003 - Tryptophan synthase beta chain like (EC 4.2.1.20), at 1,138,528 to 1,138,734
_1003
Pf1N1B4_1004 - Glyoxylate reductase (EC 1.1.1.79) / Glyoxylate reductase (EC 1.1.1.26) / Hydroxypyruvate reductase (EC 1.1.1.81); 2-ketoaldonate reductase, broad specificity (EC 1.1.1.215) (EC 1.1.1.-), at 1,138,974 to 1,139,948
_1004
Pf1N1B4_1005 - probable membrane protein YPO0899, at 1,140,285 to 1,140,650
_1005
Pf1N1B4_1006 - Permease of the drug/metabolite transporter (DMT) superfamily, at 1,140,672 to 1,141,574
_1006
Position (kb)
1138
1139
1140 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1137.997 kb on - strand, within Pf1N1B4_1002 at 1138.005 kb on + strand, within Pf1N1B4_1002 at 1138.005 kb on + strand, within Pf1N1B4_1002 at 1138.006 kb on - strand, within Pf1N1B4_1002 at 1138.021 kb on - strand, within Pf1N1B4_1002 at 1138.053 kb on + strand, within Pf1N1B4_1002 at 1138.053 kb on + strand, within Pf1N1B4_1002 at 1138.053 kb on + strand, within Pf1N1B4_1002 at 1138.054 kb on - strand, within Pf1N1B4_1002 at 1138.078 kb on + strand at 1138.078 kb on + strand at 1138.110 kb on + strand at 1138.157 kb on + strand at 1138.157 kb on + strand at 1138.170 kb on - strand at 1138.314 kb on + strand at 1138.314 kb on + strand at 1138.343 kb on + strand at 1138.486 kb on + strand at 1138.486 kb on + strand at 1138.486 kb on + strand at 1138.486 kb on + strand at 1138.486 kb on + strand at 1138.487 kb on - strand at 1138.487 kb on - strand at 1138.487 kb on - strand at 1138.487 kb on - strand at 1138.565 kb on + strand at 1138.565 kb on + strand, within Pf1N1B4_1003 at 1138.566 kb on - strand, within Pf1N1B4_1003 at 1138.573 kb on + strand, within Pf1N1B4_1003 at 1138.650 kb on + strand, within Pf1N1B4_1003 at 1138.650 kb on + strand, within Pf1N1B4_1003 at 1138.651 kb on - strand, within Pf1N1B4_1003 at 1138.651 kb on - strand at 1138.651 kb on - strand, within Pf1N1B4_1003 at 1138.651 kb on - strand, within Pf1N1B4_1003 at 1138.834 kb on + strand at 1138.834 kb on + strand at 1138.834 kb on + strand at 1138.834 kb on + strand at 1138.835 kb on - strand at 1138.835 kb on - strand at 1138.835 kb on - strand at 1138.835 kb on - strand at 1138.909 kb on - strand at 1139.211 kb on + strand, within Pf1N1B4_1004 at 1139.212 kb on - strand, within Pf1N1B4_1004 at 1139.212 kb on - strand, within Pf1N1B4_1004 at 1139.212 kb on - strand, within Pf1N1B4_1004 at 1139.224 kb on - strand, within Pf1N1B4_1004 at 1139.306 kb on + strand, within Pf1N1B4_1004 at 1139.306 kb on + strand, within Pf1N1B4_1004 at 1139.307 kb on - strand, within Pf1N1B4_1004 at 1139.307 kb on - strand, within Pf1N1B4_1004 at 1139.307 kb on - strand, within Pf1N1B4_1004 at 1139.489 kb on + strand, within Pf1N1B4_1004 at 1139.490 kb on - strand, within Pf1N1B4_1004 at 1139.490 kb on - strand, within Pf1N1B4_1004 at 1139.493 kb on + strand, within Pf1N1B4_1004 at 1139.760 kb on + strand, within Pf1N1B4_1004 at 1139.994 kb on - strand at 1140.020 kb on - strand at 1140.020 kb on - strand at 1140.020 kb on - strand at 1140.020 kb on - strand at 1140.036 kb on - strand at 1140.036 kb on - strand at 1140.036 kb on - strand at 1140.084 kb on - strand at 1140.100 kb on + strand at 1140.203 kb on - strand at 1140.229 kb on - strand at 1140.229 kb on - strand at 1140.229 kb on - strand at 1140.259 kb on - strand at 1140.259 kb on - strand at 1140.264 kb on + strand at 1140.312 kb on + strand at 1140.312 kb on + strand at 1140.313 kb on - strand at 1140.330 kb on - strand, within Pf1N1B4_1005 at 1140.415 kb on - strand, within Pf1N1B4_1005 at 1140.593 kb on + strand, within Pf1N1B4_1005 at 1140.841 kb on + strand at 1140.841 kb on + strand, within Pf1N1B4_1006 at 1140.842 kb on - strand, within Pf1N1B4_1006 at 1140.842 kb on - strand, within Pf1N1B4_1006 at 1140.922 kb on + strand, within Pf1N1B4_1006 at 1140.923 kb on - strand, within Pf1N1B4_1006 at 1140.923 kb on - strand, within Pf1N1B4_1006 at 1140.923 kb on - strand, within Pf1N1B4_1006 at 1140.940 kb on + strand, within Pf1N1B4_1006 at 1140.940 kb on + strand, within Pf1N1B4_1006 at 1140.940 kb on + strand, within Pf1N1B4_1006 at 1140.940 kb on + strand, within Pf1N1B4_1006
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,137,997 - Pf1N1B4_1002 0.84 -0.8 1,138,005 + Pf1N1B4_1002 0.85 -0.5 1,138,005 + Pf1N1B4_1002 0.85 +0.3 1,138,006 - Pf1N1B4_1002 0.85 +0.6 1,138,021 - Pf1N1B4_1002 0.86 -1.4 1,138,053 + Pf1N1B4_1002 0.89 +0.7 1,138,053 + Pf1N1B4_1002 0.89 -0.8 1,138,053 + Pf1N1B4_1002 0.89 +3.7 1,138,054 - Pf1N1B4_1002 0.90 +0.1 1,138,078 + +0.6 1,138,078 + +1.6 1,138,110 + +1.7 1,138,157 + -0.4 1,138,157 + -0.5 1,138,170 - -1.2 1,138,314 + +1.2 1,138,314 + -0.0 1,138,343 + -0.6 1,138,486 + -0.8 1,138,486 + -0.2 1,138,486 + +0.5 1,138,486 + -0.1 1,138,486 + +1.2 1,138,487 - -1.5 1,138,487 - +0.7 1,138,487 - +0.6 1,138,487 - -1.2 1,138,565 + +0.6 1,138,565 + Pf1N1B4_1003 0.18 +0.8 1,138,566 - Pf1N1B4_1003 0.18 -0.8 1,138,573 + Pf1N1B4_1003 0.22 +0.3 1,138,650 + Pf1N1B4_1003 0.59 +0.5 1,138,650 + Pf1N1B4_1003 0.59 +1.0 1,138,651 - Pf1N1B4_1003 0.59 -0.1 1,138,651 - +2.2 1,138,651 - Pf1N1B4_1003 0.59 -0.2 1,138,651 - Pf1N1B4_1003 0.59 -0.4 1,138,834 + -0.4 1,138,834 + -0.6 1,138,834 + -0.8 1,138,834 + -0.8 1,138,835 - -0.2 1,138,835 - -0.3 1,138,835 - -1.2 1,138,835 - -0.3 1,138,909 - -0.8 1,139,211 + Pf1N1B4_1004 0.24 -0.0 1,139,212 - Pf1N1B4_1004 0.24 -1.7 1,139,212 - Pf1N1B4_1004 0.24 -0.1 1,139,212 - Pf1N1B4_1004 0.24 -2.4 1,139,224 - Pf1N1B4_1004 0.26 +1.2 1,139,306 + Pf1N1B4_1004 0.34 -0.8 1,139,306 + Pf1N1B4_1004 0.34 -0.8 1,139,307 - Pf1N1B4_1004 0.34 -0.8 1,139,307 - Pf1N1B4_1004 0.34 +0.9 1,139,307 - Pf1N1B4_1004 0.34 -1.0 1,139,489 + Pf1N1B4_1004 0.53 +1.2 1,139,490 - Pf1N1B4_1004 0.53 -1.7 1,139,490 - Pf1N1B4_1004 0.53 -0.9 1,139,493 + Pf1N1B4_1004 0.53 -0.4 1,139,760 + Pf1N1B4_1004 0.81 -2.3 1,139,994 - -0.2 1,140,020 - +0.0 1,140,020 - +0.7 1,140,020 - -0.8 1,140,020 - -0.1 1,140,036 - +1.5 1,140,036 - -0.8 1,140,036 - +0.3 1,140,084 - +2.7 1,140,100 + +1.2 1,140,203 - -1.2 1,140,229 - -1.2 1,140,229 - -0.3 1,140,229 - -0.2 1,140,259 - -0.8 1,140,259 - -0.7 1,140,264 + +1.6 1,140,312 + -1.4 1,140,312 + +1.2 1,140,313 - -1.8 1,140,330 - Pf1N1B4_1005 0.12 -0.7 1,140,415 - Pf1N1B4_1005 0.36 -0.1 1,140,593 + Pf1N1B4_1005 0.84 +0.2 1,140,841 + -1.4 1,140,841 + Pf1N1B4_1006 0.19 +0.3 1,140,842 - Pf1N1B4_1006 0.19 +1.2 1,140,842 - Pf1N1B4_1006 0.19 +0.2 1,140,922 + Pf1N1B4_1006 0.28 -0.1 1,140,923 - Pf1N1B4_1006 0.28 -0.9 1,140,923 - Pf1N1B4_1006 0.28 -0.3 1,140,923 - Pf1N1B4_1006 0.28 +0.2 1,140,940 + Pf1N1B4_1006 0.30 +2.2 1,140,940 + Pf1N1B4_1006 0.30 -0.2 1,140,940 + Pf1N1B4_1006 0.30 -1.0 1,140,940 + Pf1N1B4_1006 0.30 +0.2
Or see this region's nucleotide sequence