Experiment: Hexanoic 21 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A2494 and Rru_A2495 overlap by 4 nucleotides Rru_A2495 and Rru_A2496 overlap by 4 nucleotides Rru_A2496 and Rru_A2497 are separated by 58 nucleotides
Rru_A2494: Rru_A2494 - Glycosyl transferase, family 2 (NCBI), at 2,900,277 to 2,901,245
_A2494
Rru_A2495: Rru_A2495 - Glycosyl transferase, family 2 (NCBI), at 2,901,242 to 2,902,219
_A2495
Rru_A2496: Rru_A2496 - Polysaccharide biosynthesis protein (NCBI), at 2,902,216 to 2,903,487
_A2496
Rru_A2497: Rru_A2497 - Peptide chain release factor 3 (NCBI), at 2,903,546 to 2,905,138
_A2497
Position (kb)
2902
2903
2904 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2901.886 kb on - strand, within Rru_A2495 at 2902.177 kb on - strand at 2902.260 kb on + strand at 2902.263 kb on + strand at 2902.467 kb on - strand, within Rru_A2496 at 2902.503 kb on + strand, within Rru_A2496 at 2902.734 kb on - strand, within Rru_A2496 at 2902.749 kb on + strand, within Rru_A2496 at 2902.883 kb on + strand, within Rru_A2496 at 2902.883 kb on + strand, within Rru_A2496 at 2903.019 kb on - strand, within Rru_A2496 at 2903.273 kb on + strand, within Rru_A2496 at 2903.569 kb on + strand at 2904.112 kb on + strand, within Rru_A2497 at 2904.112 kb on + strand, within Rru_A2497 at 2904.113 kb on - strand, within Rru_A2497 at 2904.113 kb on - strand, within Rru_A2497 at 2904.224 kb on - strand, within Rru_A2497 at 2904.224 kb on - strand, within Rru_A2497 at 2904.224 kb on - strand, within Rru_A2497
Per-strain Table
Position Strand Gene LocusTag Fraction Hexanoic 21 mM; anaerobic remove 2,901,886 - Rru_A2495 0.66 +0.4 2,902,177 - -2.1 2,902,260 + -0.6 2,902,263 + +2.4 2,902,467 - Rru_A2496 0.20 -1.7 2,902,503 + Rru_A2496 0.23 -1.4 2,902,734 - Rru_A2496 0.41 -1.0 2,902,749 + Rru_A2496 0.42 -0.4 2,902,883 + Rru_A2496 0.52 -3.2 2,902,883 + Rru_A2496 0.52 +1.7 2,903,019 - Rru_A2496 0.63 -0.8 2,903,273 + Rru_A2496 0.83 -1.4 2,903,569 + -0.9 2,904,112 + Rru_A2497 0.36 -2.1 2,904,112 + Rru_A2497 0.36 -3.4 2,904,113 - Rru_A2497 0.36 -3.0 2,904,113 - Rru_A2497 0.36 +1.6 2,904,224 - Rru_A2497 0.43 +0.0 2,904,224 - Rru_A2497 0.43 -2.1 2,904,224 - Rru_A2497 0.43 -4.6
Or see this region's nucleotide sequence