Experiment: Hexanoic 21 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0646 and Rru_A0647 overlap by 4 nucleotides Rru_A0647 and Rru_A0648 are separated by 52 nucleotides Rru_A0648 and Rru_A0649 are separated by 3 nucleotides
Rru_A0646: Rru_A0646 - Phosphoribosylamine--glycine ligase (NCBI), at 767,803 to 769,113
_A0646
Rru_A0647: Rru_A0647 - Pyridoxal-5'-phosphate-dependent enzyme, beta subunit (NCBI), at 769,110 to 770,102
_A0647
Rru_A0648: Rru_A0648 - hypothetical protein (NCBI), at 770,155 to 770,793
_A0648
Rru_A0649: Rru_A0649 - hypothetical protein (NCBI), at 770,797 to 771,291
_A0649
Position (kb) 769
770
771 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 768.767 kb on + strand, within Rru_A0646 at 768.767 kb on + strand, within Rru_A0646 at 768.768 kb on - strand, within Rru_A0646 at 769.567 kb on - strand, within Rru_A0647 at 769.794 kb on - strand, within Rru_A0647 at 769.794 kb on - strand, within Rru_A0647 at 770.200 kb on + strand at 770.200 kb on + strand at 770.201 kb on - strand at 770.201 kb on - strand at 770.201 kb on - strand at 770.201 kb on - strand at 770.394 kb on + strand, within Rru_A0648 at 770.394 kb on + strand, within Rru_A0648 at 770.394 kb on + strand, within Rru_A0648 at 770.394 kb on + strand, within Rru_A0648 at 770.394 kb on + strand, within Rru_A0648 at 770.394 kb on + strand, within Rru_A0648 at 770.395 kb on - strand, within Rru_A0648 at 770.395 kb on - strand, within Rru_A0648 at 770.395 kb on - strand, within Rru_A0648 at 770.396 kb on + strand, within Rru_A0648 at 770.396 kb on + strand, within Rru_A0648 at 770.396 kb on + strand, within Rru_A0648 at 770.396 kb on + strand, within Rru_A0648 at 770.397 kb on - strand, within Rru_A0648 at 770.397 kb on - strand, within Rru_A0648 at 770.397 kb on - strand, within Rru_A0648 at 770.397 kb on - strand, within Rru_A0648 at 770.519 kb on + strand, within Rru_A0648 at 770.520 kb on - strand, within Rru_A0648 at 770.520 kb on - strand, within Rru_A0648 at 770.520 kb on - strand, within Rru_A0648 at 770.520 kb on - strand, within Rru_A0648 at 770.520 kb on - strand, within Rru_A0648 at 770.571 kb on - strand, within Rru_A0648 at 770.759 kb on + strand at 770.759 kb on + strand at 770.760 kb on - strand at 770.981 kb on + strand, within Rru_A0649 at 770.981 kb on + strand, within Rru_A0649 at 770.982 kb on - strand, within Rru_A0649 at 770.982 kb on - strand, within Rru_A0649 at 771.042 kb on + strand, within Rru_A0649 at 771.043 kb on - strand, within Rru_A0649 at 771.043 kb on - strand, within Rru_A0649 at 771.074 kb on + strand, within Rru_A0649 at 771.075 kb on - strand, within Rru_A0649 at 771.075 kb on - strand, within Rru_A0649
Per-strain Table
Position Strand Gene LocusTag Fraction Hexanoic 21 mM; anaerobic remove 768,767 + Rru_A0646 0.74 -2.4 768,767 + Rru_A0646 0.74 -2.3 768,768 - Rru_A0646 0.74 -3.4 769,567 - Rru_A0647 0.46 -1.2 769,794 - Rru_A0647 0.69 -1.5 769,794 - Rru_A0647 0.69 -0.8 770,200 + -2.6 770,200 + +0.4 770,201 - -1.6 770,201 - -0.6 770,201 - -0.8 770,201 - +0.3 770,394 + Rru_A0648 0.37 -0.7 770,394 + Rru_A0648 0.37 -0.1 770,394 + Rru_A0648 0.37 -0.1 770,394 + Rru_A0648 0.37 -1.7 770,394 + Rru_A0648 0.37 +0.3 770,394 + Rru_A0648 0.37 -0.0 770,395 - Rru_A0648 0.38 +0.7 770,395 - Rru_A0648 0.38 -0.5 770,395 - Rru_A0648 0.38 -1.5 770,396 + Rru_A0648 0.38 +0.7 770,396 + Rru_A0648 0.38 -1.9 770,396 + Rru_A0648 0.38 +0.2 770,396 + Rru_A0648 0.38 -0.3 770,397 - Rru_A0648 0.38 +0.1 770,397 - Rru_A0648 0.38 -2.1 770,397 - Rru_A0648 0.38 -0.5 770,397 - Rru_A0648 0.38 +0.3 770,519 + Rru_A0648 0.57 -0.2 770,520 - Rru_A0648 0.57 -0.3 770,520 - Rru_A0648 0.57 -0.5 770,520 - Rru_A0648 0.57 -0.5 770,520 - Rru_A0648 0.57 -0.3 770,520 - Rru_A0648 0.57 -2.3 770,571 - Rru_A0648 0.65 -1.4 770,759 + -3.0 770,759 + -0.1 770,760 - -2.5 770,981 + Rru_A0649 0.37 +0.3 770,981 + Rru_A0649 0.37 -0.2 770,982 - Rru_A0649 0.37 +0.4 770,982 - Rru_A0649 0.37 -0.8 771,042 + Rru_A0649 0.49 -0.3 771,043 - Rru_A0649 0.50 +0.1 771,043 - Rru_A0649 0.50 -2.5 771,074 + Rru_A0649 0.56 -0.3 771,075 - Rru_A0649 0.56 -0.8 771,075 - Rru_A0649 0.56 -1.3
Or see this region's nucleotide sequence