Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa1541 and SMa1544 are separated by 4 nucleotides SMa1544 and SMa1545 overlap by 1 nucleotides SMa1545 and SMa1547 are separated by 294 nucleotides
SMa1541: SMa1541 - monovalent cation/H+ antiporter subunit D, at 850,844 to 852,307
SMa1541
SMa1544: SMa1544 - NuoK2 NADH:quinone oxidoreductase subunit 11 (chain K), at 852,312 to 852,614
SMa1544
SMa1545: SMa1545 - NuoJ2 NADH:quinone oxidoreductase subunit 6 (chain J), at 852,614 to 853,114
SMa1545
SMa1547: SMa1547 - protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT), at 853,409 to 854,080
SMa1547
Position (kb) 852
853 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 851.327 kb on + strand, within SMa1541 at 851.333 kb on + strand, within SMa1541 at 851.334 kb on - strand, within SMa1541 at 851.334 kb on - strand, within SMa1541 at 851.352 kb on + strand, within SMa1541 at 851.352 kb on + strand, within SMa1541 at 851.353 kb on - strand, within SMa1541 at 851.353 kb on - strand, within SMa1541 at 851.376 kb on + strand, within SMa1541 at 851.376 kb on + strand, within SMa1541 at 851.451 kb on + strand, within SMa1541 at 851.451 kb on + strand, within SMa1541 at 851.451 kb on + strand, within SMa1541 at 851.451 kb on + strand, within SMa1541 at 851.452 kb on - strand, within SMa1541 at 851.746 kb on - strand, within SMa1541 at 851.746 kb on - strand, within SMa1541 at 851.805 kb on + strand, within SMa1541 at 851.806 kb on - strand, within SMa1541 at 851.847 kb on + strand, within SMa1541 at 851.847 kb on + strand, within SMa1541 at 851.848 kb on - strand, within SMa1541 at 851.860 kb on - strand, within SMa1541 at 851.883 kb on + strand, within SMa1541 at 851.883 kb on + strand, within SMa1541 at 851.883 kb on + strand, within SMa1541 at 851.890 kb on + strand, within SMa1541 at 851.891 kb on - strand, within SMa1541 at 851.999 kb on - strand, within SMa1541 at 851.999 kb on - strand, within SMa1541 at 852.000 kb on + strand, within SMa1541 at 852.013 kb on - strand, within SMa1541 at 852.056 kb on + strand, within SMa1541 at 852.056 kb on + strand, within SMa1541 at 852.314 kb on - strand at 852.457 kb on + strand, within SMa1544 at 852.458 kb on - strand, within SMa1544 at 852.460 kb on + strand, within SMa1544 at 852.461 kb on - strand, within SMa1544 at 852.461 kb on - strand, within SMa1544 at 852.936 kb on + strand, within SMa1545 at 852.936 kb on + strand, within SMa1545 at 852.936 kb on + strand, within SMa1545 at 852.937 kb on - strand, within SMa1545 at 852.937 kb on - strand, within SMa1545 at 852.980 kb on - strand, within SMa1545 at 853.168 kb on + strand at 853.194 kb on + strand at 853.194 kb on + strand at 853.194 kb on + strand at 853.217 kb on + strand at 853.217 kb on + strand at 853.277 kb on + strand at 853.278 kb on - strand at 853.278 kb on - strand at 853.337 kb on + strand at 853.338 kb on - strand at 853.338 kb on - strand at 853.359 kb on - strand at 853.476 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 851,327 + SMa1541 0.33 +2.9 851,333 + SMa1541 0.33 +0.4 851,334 - SMa1541 0.33 -0.0 851,334 - SMa1541 0.33 -1.5 851,352 + SMa1541 0.35 -1.3 851,352 + SMa1541 0.35 +0.3 851,353 - SMa1541 0.35 +0.4 851,353 - SMa1541 0.35 -0.0 851,376 + SMa1541 0.36 -0.8 851,376 + SMa1541 0.36 -0.5 851,451 + SMa1541 0.41 +2.7 851,451 + SMa1541 0.41 -0.5 851,451 + SMa1541 0.41 +0.2 851,451 + SMa1541 0.41 +1.0 851,452 - SMa1541 0.42 +1.0 851,746 - SMa1541 0.62 +1.9 851,746 - SMa1541 0.62 +0.4 851,805 + SMa1541 0.66 +0.4 851,806 - SMa1541 0.66 -0.8 851,847 + SMa1541 0.69 -1.0 851,847 + SMa1541 0.69 -0.1 851,848 - SMa1541 0.69 +1.9 851,860 - SMa1541 0.69 -1.3 851,883 + SMa1541 0.71 -0.2 851,883 + SMa1541 0.71 +0.2 851,883 + SMa1541 0.71 -0.2 851,890 + SMa1541 0.71 +2.4 851,891 - SMa1541 0.72 -0.1 851,999 - SMa1541 0.79 -0.4 851,999 - SMa1541 0.79 +0.1 852,000 + SMa1541 0.79 -0.5 852,013 - SMa1541 0.80 -0.3 852,056 + SMa1541 0.83 +0.5 852,056 + SMa1541 0.83 -0.7 852,314 - +0.0 852,457 + SMa1544 0.48 +0.6 852,458 - SMa1544 0.48 +0.4 852,460 + SMa1544 0.49 -0.2 852,461 - SMa1544 0.49 -0.3 852,461 - SMa1544 0.49 +2.0 852,936 + SMa1545 0.64 +0.8 852,936 + SMa1545 0.64 -0.4 852,936 + SMa1545 0.64 -0.5 852,937 - SMa1545 0.64 -0.3 852,937 - SMa1545 0.64 -1.5 852,980 - SMa1545 0.73 -1.1 853,168 + -0.2 853,194 + +1.2 853,194 + +3.9 853,194 + -0.0 853,217 + -0.3 853,217 + +0.2 853,277 + -0.1 853,278 - -0.3 853,278 - -0.5 853,337 + -1.3 853,338 - +0.7 853,338 - -0.9 853,359 - +1.9 853,476 - -0.8
Or see this region's nucleotide sequence