Experiment: Bacitracin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_97 and Pf1N1B4_98 are separated by 535 nucleotides Pf1N1B4_98 and Pf1N1B4_99 are separated by 24 nucleotides
Pf1N1B4_97 - Selenide,water dikinase (EC 2.7.9.3), at 101,961 to 102,995
_97
Pf1N1B4_98 - Nucleoside-specific channel-forming protein Tsx precursor, at 103,531 to 104,487
_98
Pf1N1B4_99 - Purine nucleoside permease, at 104,512 to 105,546
_99
Position (kb) 103
104
105 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 102.722 kb on - strand, within Pf1N1B4_97 at 102.722 kb on - strand, within Pf1N1B4_97 at 102.722 kb on - strand, within Pf1N1B4_97 at 102.835 kb on + strand, within Pf1N1B4_97 at 102.836 kb on - strand, within Pf1N1B4_97 at 102.836 kb on - strand, within Pf1N1B4_97 at 102.836 kb on - strand, within Pf1N1B4_97 at 102.836 kb on - strand, within Pf1N1B4_97 at 103.032 kb on + strand at 103.033 kb on - strand at 103.076 kb on - strand at 103.225 kb on - strand at 103.379 kb on - strand at 103.398 kb on - strand at 103.529 kb on + strand at 103.705 kb on + strand, within Pf1N1B4_98 at 103.789 kb on + strand, within Pf1N1B4_98 at 103.798 kb on + strand, within Pf1N1B4_98 at 103.798 kb on + strand, within Pf1N1B4_98 at 103.799 kb on - strand, within Pf1N1B4_98 at 103.804 kb on + strand, within Pf1N1B4_98 at 103.804 kb on + strand, within Pf1N1B4_98 at 103.804 kb on + strand, within Pf1N1B4_98 at 103.805 kb on - strand, within Pf1N1B4_98 at 103.805 kb on - strand, within Pf1N1B4_98 at 103.805 kb on - strand, within Pf1N1B4_98 at 103.805 kb on - strand, within Pf1N1B4_98 at 103.805 kb on - strand at 103.834 kb on + strand, within Pf1N1B4_98 at 103.993 kb on + strand, within Pf1N1B4_98 at 103.993 kb on + strand, within Pf1N1B4_98 at 103.993 kb on + strand at 103.993 kb on + strand, within Pf1N1B4_98 at 103.994 kb on - strand, within Pf1N1B4_98 at 103.994 kb on - strand, within Pf1N1B4_98 at 104.014 kb on + strand, within Pf1N1B4_98 at 104.014 kb on + strand, within Pf1N1B4_98 at 104.015 kb on - strand, within Pf1N1B4_98 at 104.015 kb on - strand, within Pf1N1B4_98 at 104.056 kb on + strand, within Pf1N1B4_98 at 104.056 kb on + strand at 104.056 kb on + strand, within Pf1N1B4_98 at 104.057 kb on - strand, within Pf1N1B4_98 at 104.116 kb on + strand, within Pf1N1B4_98 at 104.159 kb on - strand, within Pf1N1B4_98 at 104.191 kb on + strand, within Pf1N1B4_98 at 104.191 kb on + strand, within Pf1N1B4_98 at 104.191 kb on + strand at 104.192 kb on - strand, within Pf1N1B4_98 at 104.192 kb on - strand, within Pf1N1B4_98 at 104.192 kb on - strand, within Pf1N1B4_98 at 104.192 kb on - strand, within Pf1N1B4_98 at 104.192 kb on - strand, within Pf1N1B4_98 at 104.236 kb on + strand, within Pf1N1B4_98 at 104.236 kb on + strand, within Pf1N1B4_98 at 104.236 kb on + strand, within Pf1N1B4_98 at 104.236 kb on + strand, within Pf1N1B4_98 at 104.237 kb on - strand, within Pf1N1B4_98 at 104.332 kb on + strand, within Pf1N1B4_98 at 104.461 kb on + strand at 104.485 kb on + strand at 104.485 kb on + strand at 104.512 kb on - strand at 104.715 kb on + strand, within Pf1N1B4_99 at 104.830 kb on + strand, within Pf1N1B4_99 at 104.902 kb on + strand, within Pf1N1B4_99 at 104.902 kb on + strand, within Pf1N1B4_99 at 104.903 kb on - strand, within Pf1N1B4_99 at 104.903 kb on - strand at 105.020 kb on - strand, within Pf1N1B4_99 at 105.192 kb on + strand, within Pf1N1B4_99 at 105.192 kb on + strand, within Pf1N1B4_99 at 105.255 kb on + strand, within Pf1N1B4_99 at 105.268 kb on + strand, within Pf1N1B4_99 at 105.268 kb on + strand, within Pf1N1B4_99 at 105.269 kb on - strand, within Pf1N1B4_99 at 105.301 kb on + strand, within Pf1N1B4_99 at 105.301 kb on + strand, within Pf1N1B4_99 at 105.301 kb on + strand, within Pf1N1B4_99 at 105.301 kb on + strand, within Pf1N1B4_99 at 105.301 kb on + strand, within Pf1N1B4_99 at 105.302 kb on - strand at 105.302 kb on - strand, within Pf1N1B4_99 at 105.428 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 2 mg/ml remove 102,722 - Pf1N1B4_97 0.74 -2.2 102,722 - Pf1N1B4_97 0.74 +1.4 102,722 - Pf1N1B4_97 0.74 +1.1 102,835 + Pf1N1B4_97 0.84 +0.2 102,836 - Pf1N1B4_97 0.85 +0.8 102,836 - Pf1N1B4_97 0.85 -0.2 102,836 - Pf1N1B4_97 0.85 +0.3 102,836 - Pf1N1B4_97 0.85 -2.6 103,032 + -1.0 103,033 - -1.6 103,076 - +0.6 103,225 - -1.9 103,379 - +0.8 103,398 - +1.0 103,529 + +0.2 103,705 + Pf1N1B4_98 0.18 +0.2 103,789 + Pf1N1B4_98 0.27 +0.2 103,798 + Pf1N1B4_98 0.28 -1.0 103,798 + Pf1N1B4_98 0.28 -1.8 103,799 - Pf1N1B4_98 0.28 -0.9 103,804 + Pf1N1B4_98 0.29 +0.4 103,804 + Pf1N1B4_98 0.29 +1.4 103,804 + Pf1N1B4_98 0.29 +1.5 103,805 - Pf1N1B4_98 0.29 +0.1 103,805 - Pf1N1B4_98 0.29 -0.4 103,805 - Pf1N1B4_98 0.29 -0.4 103,805 - Pf1N1B4_98 0.29 +0.2 103,805 - -1.2 103,834 + Pf1N1B4_98 0.32 +0.8 103,993 + Pf1N1B4_98 0.48 -0.5 103,993 + Pf1N1B4_98 0.48 -1.6 103,993 + +0.8 103,993 + Pf1N1B4_98 0.48 -0.3 103,994 - Pf1N1B4_98 0.48 -0.2 103,994 - Pf1N1B4_98 0.48 +0.2 104,014 + Pf1N1B4_98 0.50 -0.2 104,014 + Pf1N1B4_98 0.50 -0.8 104,015 - Pf1N1B4_98 0.51 -0.2 104,015 - Pf1N1B4_98 0.51 -1.7 104,056 + Pf1N1B4_98 0.55 +0.8 104,056 + +0.8 104,056 + Pf1N1B4_98 0.55 -2.4 104,057 - Pf1N1B4_98 0.55 -0.8 104,116 + Pf1N1B4_98 0.61 +0.8 104,159 - Pf1N1B4_98 0.66 +0.2 104,191 + Pf1N1B4_98 0.69 -0.0 104,191 + Pf1N1B4_98 0.69 +0.6 104,191 + -0.2 104,192 - Pf1N1B4_98 0.69 +0.8 104,192 - Pf1N1B4_98 0.69 -1.5 104,192 - Pf1N1B4_98 0.69 -0.4 104,192 - Pf1N1B4_98 0.69 -2.0 104,192 - Pf1N1B4_98 0.69 -0.6 104,236 + Pf1N1B4_98 0.74 +0.8 104,236 + Pf1N1B4_98 0.74 -0.6 104,236 + Pf1N1B4_98 0.74 +1.3 104,236 + Pf1N1B4_98 0.74 -1.0 104,237 - Pf1N1B4_98 0.74 +1.0 104,332 + Pf1N1B4_98 0.84 -1.2 104,461 + -0.6 104,485 + -1.5 104,485 + +0.5 104,512 - -1.3 104,715 + Pf1N1B4_99 0.20 -1.6 104,830 + Pf1N1B4_99 0.31 +0.4 104,902 + Pf1N1B4_99 0.38 +1.0 104,902 + Pf1N1B4_99 0.38 +1.6 104,903 - Pf1N1B4_99 0.38 -0.6 104,903 - +3.1 105,020 - Pf1N1B4_99 0.49 +0.6 105,192 + Pf1N1B4_99 0.66 -0.0 105,192 + Pf1N1B4_99 0.66 -2.4 105,255 + Pf1N1B4_99 0.72 +0.9 105,268 + Pf1N1B4_99 0.73 -1.1 105,268 + Pf1N1B4_99 0.73 -0.8 105,269 - Pf1N1B4_99 0.73 +1.1 105,301 + Pf1N1B4_99 0.76 -0.6 105,301 + Pf1N1B4_99 0.76 +0.2 105,301 + Pf1N1B4_99 0.76 +0.1 105,301 + Pf1N1B4_99 0.76 -1.6 105,301 + Pf1N1B4_99 0.76 +2.2 105,302 - -1.6 105,302 - Pf1N1B4_99 0.76 +0.8 105,428 - +0.2
Or see this region's nucleotide sequence