Experiment: Bacitracin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5892 and Pf1N1B4_5893 overlap by 4 nucleotides Pf1N1B4_5893 and Pf1N1B4_5894 overlap by 8 nucleotides Pf1N1B4_5894 and Pf1N1B4_5895 are separated by 12 nucleotides
Pf1N1B4_5892 - FIG00961753: hypothetical protein, at 336,034 to 336,216
_5892
Pf1N1B4_5893 - Mycofactocin system glycosyltransferase, at 336,213 to 337,190
_5893
Pf1N1B4_5894 - Glycosyltransferase, at 337,183 to 338,319
_5894
Pf1N1B4_5895 - FIG00958408: hypothetical protein, at 338,332 to 339,333
_5895
Position (kb) 337
338
339 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 336.252 kb on + strand at 336.252 kb on + strand at 336.279 kb on - strand at 337.015 kb on + strand, within Pf1N1B4_5893 at 337.016 kb on - strand, within Pf1N1B4_5893 at 337.078 kb on + strand, within Pf1N1B4_5893 at 337.079 kb on - strand at 337.079 kb on - strand, within Pf1N1B4_5893 at 337.225 kb on + strand at 337.225 kb on + strand at 337.225 kb on + strand at 337.226 kb on - strand at 337.226 kb on - strand at 337.364 kb on + strand, within Pf1N1B4_5894 at 337.712 kb on - strand, within Pf1N1B4_5894 at 337.876 kb on + strand, within Pf1N1B4_5894 at 337.877 kb on - strand, within Pf1N1B4_5894 at 338.018 kb on - strand, within Pf1N1B4_5894 at 338.018 kb on - strand at 338.979 kb on - strand, within Pf1N1B4_5895
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 2 mg/ml remove 336,252 + +0.6 336,252 + -0.2 336,279 - +0.2 337,015 + Pf1N1B4_5893 0.82 -3.0 337,016 - Pf1N1B4_5893 0.82 +0.6 337,078 + Pf1N1B4_5893 0.88 -0.6 337,079 - +1.6 337,079 - Pf1N1B4_5893 0.89 -0.4 337,225 + -0.1 337,225 + +0.9 337,225 + -0.1 337,226 - -1.4 337,226 - -0.6 337,364 + Pf1N1B4_5894 0.16 +0.6 337,712 - Pf1N1B4_5894 0.47 -2.0 337,876 + Pf1N1B4_5894 0.61 -0.4 337,877 - Pf1N1B4_5894 0.61 -1.2 338,018 - Pf1N1B4_5894 0.73 -1.4 338,018 - -1.0 338,979 - Pf1N1B4_5895 0.65 -1.0
Or see this region's nucleotide sequence