Experiment: Bacitracin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5820 and Pf1N1B4_5821 overlap by 6 nucleotides Pf1N1B4_5821 and Pf1N1B4_5822 are separated by 290 nucleotides
Pf1N1B4_5820 - Transcriptional regulator, Cro/CI family, at 254,204 to 254,767
_5820
Pf1N1B4_5821 - Permease of the drug/metabolite transporter (DMT) superfamily, at 254,762 to 255,790
_5821
Pf1N1B4_5822 - Na(+) H(+) antiporter subunit A / Na(+) H(+) antiporter subunit B, at 256,081 to 258,957
_5822
Position (kb) 254
255
256 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 253.772 kb on - strand at 253.887 kb on - strand at 253.891 kb on + strand at 253.892 kb on - strand at 253.892 kb on - strand at 253.892 kb on - strand at 253.892 kb on - strand at 253.913 kb on - strand at 253.913 kb on - strand at 253.913 kb on - strand at 253.913 kb on - strand at 253.914 kb on + strand at 253.914 kb on + strand at 253.915 kb on - strand at 253.915 kb on - strand at 253.915 kb on - strand at 254.014 kb on - strand at 254.069 kb on - strand at 254.069 kb on - strand at 254.069 kb on - strand at 254.069 kb on - strand at 254.069 kb on - strand at 254.069 kb on - strand at 254.151 kb on - strand at 254.169 kb on + strand at 254.169 kb on + strand at 254.170 kb on - strand at 254.170 kb on - strand at 254.170 kb on - strand at 254.172 kb on - strand at 254.172 kb on - strand at 254.172 kb on - strand at 254.172 kb on - strand at 254.172 kb on - strand at 254.173 kb on + strand at 254.173 kb on + strand at 254.173 kb on + strand at 254.174 kb on - strand at 254.174 kb on - strand at 254.174 kb on - strand at 254.174 kb on - strand at 254.174 kb on - strand at 254.273 kb on - strand, within Pf1N1B4_5820 at 254.285 kb on - strand, within Pf1N1B4_5820 at 254.355 kb on + strand, within Pf1N1B4_5820 at 254.356 kb on - strand, within Pf1N1B4_5820 at 254.356 kb on - strand, within Pf1N1B4_5820 at 254.356 kb on - strand, within Pf1N1B4_5820 at 254.369 kb on - strand, within Pf1N1B4_5820 at 254.371 kb on - strand, within Pf1N1B4_5820 at 254.564 kb on + strand, within Pf1N1B4_5820 at 254.770 kb on + strand at 254.799 kb on + strand at 254.932 kb on + strand, within Pf1N1B4_5821 at 254.932 kb on + strand, within Pf1N1B4_5821 at 254.933 kb on - strand, within Pf1N1B4_5821 at 255.057 kb on + strand, within Pf1N1B4_5821 at 255.188 kb on + strand, within Pf1N1B4_5821 at 255.188 kb on + strand, within Pf1N1B4_5821 at 255.188 kb on + strand, within Pf1N1B4_5821 at 255.188 kb on + strand, within Pf1N1B4_5821 at 255.392 kb on + strand at 255.392 kb on + strand, within Pf1N1B4_5821 at 255.392 kb on + strand, within Pf1N1B4_5821 at 255.392 kb on + strand, within Pf1N1B4_5821 at 255.393 kb on - strand, within Pf1N1B4_5821 at 255.411 kb on + strand, within Pf1N1B4_5821 at 255.411 kb on + strand, within Pf1N1B4_5821 at 255.412 kb on - strand, within Pf1N1B4_5821 at 255.611 kb on + strand, within Pf1N1B4_5821 at 255.611 kb on + strand, within Pf1N1B4_5821 at 255.945 kb on - strand at 255.991 kb on + strand at 255.991 kb on + strand at 256.018 kb on + strand at 256.026 kb on + strand at 256.037 kb on + strand at 256.037 kb on + strand at 256.044 kb on - strand at 256.321 kb on + strand at 256.321 kb on + strand at 256.322 kb on - strand at 256.322 kb on - strand at 256.322 kb on - strand at 256.507 kb on + strand, within Pf1N1B4_5822 at 256.507 kb on + strand, within Pf1N1B4_5822 at 256.508 kb on - strand, within Pf1N1B4_5822 at 256.588 kb on + strand, within Pf1N1B4_5822
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 2 mg/ml remove 253,772 - -1.6 253,887 - +0.6 253,891 + -0.4 253,892 - +0.3 253,892 - +1.2 253,892 - -1.7 253,892 - +0.3 253,913 - -0.6 253,913 - -0.4 253,913 - -1.0 253,913 - -0.9 253,914 + -2.0 253,914 + +0.6 253,915 - -0.2 253,915 - +0.6 253,915 - -0.7 254,014 - -1.2 254,069 - +0.6 254,069 - +0.6 254,069 - -0.2 254,069 - +0.8 254,069 - +2.2 254,069 - +0.2 254,151 - +1.0 254,169 + +0.2 254,169 + -1.4 254,170 - -2.4 254,170 - -0.3 254,170 - -2.2 254,172 - -1.8 254,172 - -0.1 254,172 - +0.9 254,172 - +0.6 254,172 - -2.0 254,173 + +0.1 254,173 + +0.3 254,173 + +0.4 254,174 - -0.6 254,174 - +0.1 254,174 - +0.6 254,174 - -0.2 254,174 - -2.2 254,273 - Pf1N1B4_5820 0.12 +0.6 254,285 - Pf1N1B4_5820 0.14 -0.4 254,355 + Pf1N1B4_5820 0.27 +1.3 254,356 - Pf1N1B4_5820 0.27 +0.7 254,356 - Pf1N1B4_5820 0.27 -0.8 254,356 - Pf1N1B4_5820 0.27 +0.1 254,369 - Pf1N1B4_5820 0.29 -0.4 254,371 - Pf1N1B4_5820 0.30 -0.3 254,564 + Pf1N1B4_5820 0.64 +1.3 254,770 + -0.1 254,799 + -1.0 254,932 + Pf1N1B4_5821 0.17 -0.2 254,932 + Pf1N1B4_5821 0.17 -2.2 254,933 - Pf1N1B4_5821 0.17 -0.7 255,057 + Pf1N1B4_5821 0.29 +0.1 255,188 + Pf1N1B4_5821 0.41 -1.7 255,188 + Pf1N1B4_5821 0.41 +0.3 255,188 + Pf1N1B4_5821 0.41 -1.6 255,188 + Pf1N1B4_5821 0.41 -0.4 255,392 + +3.2 255,392 + Pf1N1B4_5821 0.61 +0.9 255,392 + Pf1N1B4_5821 0.61 +0.7 255,392 + Pf1N1B4_5821 0.61 -0.4 255,393 - Pf1N1B4_5821 0.61 -1.0 255,411 + Pf1N1B4_5821 0.63 -0.9 255,411 + Pf1N1B4_5821 0.63 -0.6 255,412 - Pf1N1B4_5821 0.63 +0.4 255,611 + Pf1N1B4_5821 0.83 -1.6 255,611 + Pf1N1B4_5821 0.83 -1.2 255,945 - -1.4 255,991 + -1.0 255,991 + -1.3 256,018 + +0.6 256,026 + +0.7 256,037 + +0.0 256,037 + -1.4 256,044 - +0.6 256,321 + +0.3 256,321 + +0.4 256,322 - +0.0 256,322 - +0.6 256,322 - +0.4 256,507 + Pf1N1B4_5822 0.15 -1.4 256,507 + Pf1N1B4_5822 0.15 -3.0 256,508 - Pf1N1B4_5822 0.15 -2.0 256,588 + Pf1N1B4_5822 0.18 -0.1
Or see this region's nucleotide sequence