Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5715

Experiment: Bacitracin 2 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5714 and Pf1N1B4_5715 are separated by 108 nucleotidesPf1N1B4_5715 and Pf1N1B4_5716 overlap by 4 nucleotides Pf1N1B4_5714 - RND efflux system, outer membrane lipoprotein, NodT family, at 155,007 to 156,611 _5714 Pf1N1B4_5715 - RND efflux membrane fusion protein, at 156,720 to 157,754 _5715 Pf1N1B4_5716 - Acriflavin resistance protein, at 157,751 to 160,846 _5716 Position (kb) 156 157 158Strain fitness (log2 ratio) -2 -1 0 1 2 3at 156.019 kb on + strand, within Pf1N1B4_5714at 156.522 kb on + strandat 156.522 kb on + strandat 156.523 kb on - strandat 156.523 kb on - strandat 156.523 kb on - strandat 156.523 kb on - strandat 156.523 kb on - strandat 156.799 kb on + strandat 156.868 kb on + strand, within Pf1N1B4_5715at 157.061 kb on + strand, within Pf1N1B4_5715at 157.062 kb on - strand, within Pf1N1B4_5715at 157.062 kb on - strand, within Pf1N1B4_5715at 157.062 kb on - strand, within Pf1N1B4_5715at 157.062 kb on - strand, within Pf1N1B4_5715at 157.137 kb on + strand, within Pf1N1B4_5715at 157.137 kb on + strand, within Pf1N1B4_5715at 157.138 kb on - strand, within Pf1N1B4_5715at 157.138 kb on - strandat 157.396 kb on - strand, within Pf1N1B4_5715at 157.691 kb on + strandat 158.174 kb on + strand, within Pf1N1B4_5716at 158.570 kb on + strand, within Pf1N1B4_5716at 158.570 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bacitracin 2 mg/ml
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156,019 + Pf1N1B4_5714 0.63 +1.0
156,522 + +1.6
156,522 + +2.9
156,523 - -0.7
156,523 - -1.0
156,523 - +0.6
156,523 - -0.2
156,523 - +1.9
156,799 + -2.2
156,868 + Pf1N1B4_5715 0.14 -0.0
157,061 + Pf1N1B4_5715 0.33 -1.2
157,062 - Pf1N1B4_5715 0.33 -1.6
157,062 - Pf1N1B4_5715 0.33 -1.9
157,062 - Pf1N1B4_5715 0.33 -1.2
157,062 - Pf1N1B4_5715 0.33 -0.4
157,137 + Pf1N1B4_5715 0.40 -0.4
157,137 + Pf1N1B4_5715 0.40 +0.6
157,138 - Pf1N1B4_5715 0.40 -1.1
157,138 - +0.6
157,396 - Pf1N1B4_5715 0.65 -1.0
157,691 + +0.3
158,174 + Pf1N1B4_5716 0.14 -1.9
158,570 + Pf1N1B4_5716 0.26 -1.0
158,570 + -0.4

Or see this region's nucleotide sequence