Experiment: Bacitracin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_514 and Pf1N1B4_515 are separated by 256 nucleotides Pf1N1B4_515 and Pf1N1B4_516 overlap by 1 nucleotides
Pf1N1B4_514 - Orotidine 5'-phosphate decarboxylase (EC 4.1.1.23), at 605,492 to 606,226
_514
Pf1N1B4_515 - DNA-binding response regulator GltR, controls specific porins for the entry of glucose, at 606,483 to 607,241
_515
Pf1N1B4_516 - Sensor histidine kinase, at 607,241 to 608,293
_516
Position (kb) 606
607
608 Strain fitness (log2 ratio) -1
0
1
2 at 605.483 kb on + strand at 605.493 kb on + strand at 606.294 kb on + strand at 606.294 kb on + strand at 606.295 kb on - strand at 606.415 kb on + strand at 606.416 kb on - strand at 606.441 kb on + strand at 606.441 kb on + strand at 606.441 kb on + strand at 606.442 kb on - strand at 606.461 kb on + strand at 606.461 kb on + strand at 606.461 kb on + strand at 606.536 kb on + strand at 606.604 kb on - strand, within Pf1N1B4_515 at 606.621 kb on - strand, within Pf1N1B4_515 at 606.837 kb on + strand, within Pf1N1B4_515 at 606.837 kb on + strand, within Pf1N1B4_515 at 606.837 kb on + strand, within Pf1N1B4_515 at 606.837 kb on + strand, within Pf1N1B4_515 at 606.838 kb on - strand, within Pf1N1B4_515 at 606.838 kb on - strand, within Pf1N1B4_515 at 606.838 kb on - strand at 606.838 kb on - strand, within Pf1N1B4_515 at 606.838 kb on - strand, within Pf1N1B4_515 at 606.955 kb on + strand at 607.213 kb on - strand at 607.213 kb on - strand at 607.258 kb on + strand at 607.302 kb on + strand at 607.302 kb on + strand at 607.303 kb on - strand at 607.832 kb on + strand, within Pf1N1B4_516 at 607.832 kb on + strand, within Pf1N1B4_516 at 607.832 kb on + strand at 607.832 kb on + strand, within Pf1N1B4_516 at 607.833 kb on - strand at 607.833 kb on - strand, within Pf1N1B4_516 at 608.158 kb on + strand, within Pf1N1B4_516 at 608.158 kb on + strand, within Pf1N1B4_516
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 2 mg/ml remove 605,483 + -0.5 605,493 + -0.6 606,294 + -0.7 606,294 + -1.4 606,295 - -0.3 606,415 + -1.4 606,416 - -0.9 606,441 + -1.7 606,441 + -0.6 606,441 + +0.5 606,442 - -0.1 606,461 + -0.3 606,461 + +0.2 606,461 + -0.9 606,536 + -0.3 606,604 - Pf1N1B4_515 0.16 -1.1 606,621 - Pf1N1B4_515 0.18 -1.0 606,837 + Pf1N1B4_515 0.47 -0.7 606,837 + Pf1N1B4_515 0.47 -1.7 606,837 + Pf1N1B4_515 0.47 +0.2 606,837 + Pf1N1B4_515 0.47 +1.1 606,838 - Pf1N1B4_515 0.47 -0.1 606,838 - Pf1N1B4_515 0.47 -1.4 606,838 - -0.1 606,838 - Pf1N1B4_515 0.47 +0.3 606,838 - Pf1N1B4_515 0.47 -1.1 606,955 + +0.9 607,213 - -0.6 607,213 - +0.9 607,258 + +0.9 607,302 + +1.9 607,302 + +0.3 607,303 - +1.1 607,832 + Pf1N1B4_516 0.56 +0.3 607,832 + Pf1N1B4_516 0.56 -0.7 607,832 + +0.9 607,832 + Pf1N1B4_516 0.56 +0.4 607,833 - -0.1 607,833 - Pf1N1B4_516 0.56 +0.8 608,158 + Pf1N1B4_516 0.87 -1.2 608,158 + Pf1N1B4_516 0.87 +0.4
Or see this region's nucleotide sequence