Experiment: Bacitracin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5034 and Pf1N1B4_5035 are separated by 306 nucleotides Pf1N1B4_5035 and Pf1N1B4_5036 are separated by 233 nucleotides Pf1N1B4_5036 and Pf1N1B4_5236 are separated by 165 nucleotides Pf1N1B4_5236 and Pf1N1B4_5237 are separated by 198 nucleotides Pf1N1B4_5237 and Pf1N1B4_5037 are separated by 282 nucleotides
Pf1N1B4_5034 - hypothetical protein, at 5,579,569 to 5,579,799
_5034
Pf1N1B4_5035 - hypothetical protein, at 5,580,106 to 5,580,390
_5035
Pf1N1B4_5036 - hypothetical protein, at 5,580,624 to 5,580,863
_5036
Pf1N1B4_5236 - tRNA-Leu-TAA, at 5,581,029 to 5,581,112
_5236
Pf1N1B4_5237 - tRNA-Cys-GCA, at 5,581,311 to 5,581,381
_5237
Pf1N1B4_5037 - Transcriptional regulator of various polyols utilization, AraC family, at 5,581,664 to 5,582,572
_5037
Position (kb)
5580
5581 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5579.772 kb on - strand, within Pf1N1B4_5034 at 5579.875 kb on + strand at 5579.876 kb on - strand at 5579.876 kb on - strand at 5580.044 kb on + strand at 5580.044 kb on + strand at 5580.044 kb on + strand at 5580.045 kb on - strand at 5580.045 kb on - strand at 5580.179 kb on + strand, within Pf1N1B4_5035 at 5580.180 kb on - strand, within Pf1N1B4_5035 at 5580.180 kb on - strand at 5580.388 kb on + strand at 5580.434 kb on - strand at 5580.434 kb on - strand at 5580.562 kb on - strand at 5580.590 kb on + strand at 5580.590 kb on + strand at 5580.590 kb on + strand at 5580.786 kb on + strand at 5580.786 kb on + strand, within Pf1N1B4_5036 at 5580.787 kb on - strand, within Pf1N1B4_5036 at 5580.787 kb on - strand, within Pf1N1B4_5036 at 5580.787 kb on - strand at 5580.787 kb on - strand, within Pf1N1B4_5036 at 5580.787 kb on - strand, within Pf1N1B4_5036 at 5580.789 kb on + strand, within Pf1N1B4_5036 at 5580.789 kb on + strand, within Pf1N1B4_5036 at 5580.789 kb on + strand, within Pf1N1B4_5036 at 5580.789 kb on + strand, within Pf1N1B4_5036 at 5580.790 kb on - strand, within Pf1N1B4_5036 at 5580.790 kb on - strand, within Pf1N1B4_5036 at 5580.793 kb on - strand, within Pf1N1B4_5036 at 5580.861 kb on + strand at 5580.861 kb on + strand at 5580.861 kb on + strand at 5580.897 kb on - strand at 5581.191 kb on - strand at 5581.191 kb on - strand at 5581.191 kb on - strand at 5581.195 kb on - strand at 5581.278 kb on - strand at 5581.786 kb on + strand, within Pf1N1B4_5037
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 2 mg/ml remove 5,579,772 - Pf1N1B4_5034 0.88 -1.1 5,579,875 + -0.0 5,579,876 - -0.7 5,579,876 - -0.1 5,580,044 + -0.1 5,580,044 + -0.1 5,580,044 + -0.4 5,580,045 - +0.1 5,580,045 - +0.5 5,580,179 + Pf1N1B4_5035 0.26 -1.5 5,580,180 - Pf1N1B4_5035 0.26 +1.2 5,580,180 - -0.9 5,580,388 + -1.1 5,580,434 - +1.6 5,580,434 - -1.9 5,580,562 - +0.5 5,580,590 + -1.9 5,580,590 + -0.0 5,580,590 + +1.7 5,580,786 + +1.0 5,580,786 + Pf1N1B4_5036 0.68 -0.7 5,580,787 - Pf1N1B4_5036 0.68 -1.9 5,580,787 - Pf1N1B4_5036 0.68 -1.7 5,580,787 - -0.5 5,580,787 - Pf1N1B4_5036 0.68 +0.1 5,580,787 - Pf1N1B4_5036 0.68 -2.9 5,580,789 + Pf1N1B4_5036 0.69 +1.0 5,580,789 + Pf1N1B4_5036 0.69 -1.1 5,580,789 + Pf1N1B4_5036 0.69 -0.3 5,580,789 + Pf1N1B4_5036 0.69 -1.3 5,580,790 - Pf1N1B4_5036 0.69 +1.0 5,580,790 - Pf1N1B4_5036 0.69 -0.8 5,580,793 - Pf1N1B4_5036 0.70 -2.1 5,580,861 + +1.5 5,580,861 + -0.5 5,580,861 + +0.5 5,580,897 - -1.0 5,581,191 - -0.5 5,581,191 - -1.1 5,581,191 - +1.7 5,581,195 - +0.6 5,581,278 - -1.0 5,581,786 + Pf1N1B4_5037 0.13 -0.5
Or see this region's nucleotide sequence