Experiment: Bacitracin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4989 and Pf1N1B4_4990 are separated by 333 nucleotides Pf1N1B4_4990 and Pf1N1B4_4991 are separated by 137 nucleotides
Pf1N1B4_4989 - Thymidylate kinase (EC 2.7.4.9), at 5,536,159 to 5,536,353
_4989
Pf1N1B4_4990 - FIG018171: hypothetical protein of Cupin superfamily, at 5,536,687 to 5,537,211
_4990
Pf1N1B4_4991 - Transcriptional regulator, AraC family, at 5,537,349 to 5,537,834
_4991
Position (kb)
5536
5537
5538 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5535.734 kb on + strand at 5535.735 kb on - strand at 5535.967 kb on - strand at 5536.056 kb on - strand at 5536.247 kb on + strand, within Pf1N1B4_4989 at 5536.303 kb on + strand, within Pf1N1B4_4989 at 5536.357 kb on + strand at 5536.357 kb on + strand at 5536.357 kb on + strand at 5536.361 kb on + strand at 5536.477 kb on - strand at 5536.477 kb on - strand at 5536.623 kb on + strand at 5536.864 kb on + strand at 5536.917 kb on + strand, within Pf1N1B4_4990 at 5536.976 kb on + strand, within Pf1N1B4_4990 at 5536.977 kb on - strand, within Pf1N1B4_4990 at 5537.014 kb on + strand, within Pf1N1B4_4990 at 5537.015 kb on - strand, within Pf1N1B4_4990 at 5537.299 kb on - strand at 5537.313 kb on + strand at 5537.350 kb on + strand at 5537.352 kb on + strand at 5537.352 kb on + strand at 5537.352 kb on + strand at 5537.352 kb on + strand at 5537.416 kb on + strand, within Pf1N1B4_4991 at 5537.700 kb on + strand, within Pf1N1B4_4991 at 5537.701 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 2 mg/ml remove 5,535,734 + +1.2 5,535,735 - -0.1 5,535,967 - -0.3 5,536,056 - +0.8 5,536,247 + Pf1N1B4_4989 0.45 +1.8 5,536,303 + Pf1N1B4_4989 0.74 -0.5 5,536,357 + -0.1 5,536,357 + -0.3 5,536,357 + -1.1 5,536,361 + -0.8 5,536,477 - +0.5 5,536,477 - -3.0 5,536,623 + +0.5 5,536,864 + +2.0 5,536,917 + Pf1N1B4_4990 0.44 +0.1 5,536,976 + Pf1N1B4_4990 0.55 -0.8 5,536,977 - Pf1N1B4_4990 0.55 -0.7 5,537,014 + Pf1N1B4_4990 0.62 -1.7 5,537,015 - Pf1N1B4_4990 0.62 -0.5 5,537,299 - -1.5 5,537,313 + +1.5 5,537,350 + -0.0 5,537,352 + -0.5 5,537,352 + +1.0 5,537,352 + +0.6 5,537,352 + -0.6 5,537,416 + Pf1N1B4_4991 0.14 +2.3 5,537,700 + Pf1N1B4_4991 0.72 -0.8 5,537,701 - +1.5
Or see this region's nucleotide sequence