Experiment: Bacitracin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4849 and Pf1N1B4_4850 are separated by 163 nucleotides Pf1N1B4_4850 and Pf1N1B4_4851 are separated by 338 nucleotides
Pf1N1B4_4849 - Various polyols ABC transporter, permease component 1, at 5,357,837 to 5,358,736
_4849
Pf1N1B4_4850 - Various polyols ABC transporter, periplasmic substrate-binding protein, at 5,358,900 to 5,360,222
_4850
Pf1N1B4_4851 - Transcriptional regulator of various polyols utilization, AraC family, at 5,360,561 to 5,361,424
_4851
Position (kb) 5358
5359
5360
5361 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5357.976 kb on + strand, within Pf1N1B4_4849 at 5358.306 kb on + strand, within Pf1N1B4_4849 at 5358.307 kb on - strand, within Pf1N1B4_4849 at 5358.712 kb on + strand at 5358.757 kb on + strand at 5358.780 kb on - strand at 5358.835 kb on + strand at 5358.835 kb on + strand at 5358.836 kb on - strand at 5358.836 kb on - strand at 5358.902 kb on - strand at 5358.902 kb on - strand at 5358.913 kb on - strand at 5359.079 kb on - strand, within Pf1N1B4_4850 at 5359.163 kb on + strand, within Pf1N1B4_4850 at 5359.163 kb on + strand, within Pf1N1B4_4850 at 5359.164 kb on - strand, within Pf1N1B4_4850 at 5359.164 kb on - strand at 5359.164 kb on - strand at 5359.165 kb on + strand, within Pf1N1B4_4850 at 5359.165 kb on + strand, within Pf1N1B4_4850 at 5359.166 kb on - strand, within Pf1N1B4_4850 at 5359.166 kb on - strand at 5359.166 kb on - strand, within Pf1N1B4_4850 at 5359.178 kb on - strand, within Pf1N1B4_4850 at 5359.244 kb on - strand at 5359.328 kb on - strand, within Pf1N1B4_4850 at 5359.328 kb on - strand, within Pf1N1B4_4850 at 5359.838 kb on - strand, within Pf1N1B4_4850 at 5359.898 kb on - strand, within Pf1N1B4_4850 at 5359.960 kb on + strand, within Pf1N1B4_4850 at 5360.000 kb on + strand, within Pf1N1B4_4850 at 5360.000 kb on + strand, within Pf1N1B4_4850 at 5360.266 kb on - strand at 5360.308 kb on + strand at 5360.308 kb on + strand at 5360.432 kb on + strand at 5360.432 kb on + strand at 5360.504 kb on + strand at 5360.504 kb on + strand at 5360.504 kb on + strand at 5360.504 kb on + strand at 5360.504 kb on + strand at 5360.504 kb on + strand at 5360.505 kb on - strand at 5360.505 kb on - strand at 5360.505 kb on - strand at 5360.505 kb on - strand at 5360.506 kb on + strand at 5360.506 kb on + strand at 5360.506 kb on + strand at 5360.506 kb on + strand at 5360.506 kb on + strand at 5360.506 kb on + strand at 5360.506 kb on + strand at 5360.506 kb on + strand at 5360.506 kb on + strand at 5360.506 kb on + strand at 5360.507 kb on - strand at 5360.508 kb on + strand at 5360.555 kb on + strand at 5360.559 kb on + strand at 5360.560 kb on - strand at 5360.560 kb on - strand at 5360.663 kb on - strand, within Pf1N1B4_4851 at 5360.663 kb on - strand, within Pf1N1B4_4851 at 5360.663 kb on - strand, within Pf1N1B4_4851 at 5360.877 kb on + strand, within Pf1N1B4_4851 at 5360.955 kb on + strand, within Pf1N1B4_4851
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 2 mg/ml remove 5,357,976 + Pf1N1B4_4849 0.15 -0.9 5,358,306 + Pf1N1B4_4849 0.52 -0.5 5,358,307 - Pf1N1B4_4849 0.52 -0.0 5,358,712 + +0.6 5,358,757 + +0.4 5,358,780 - -0.8 5,358,835 + -0.9 5,358,835 + +1.2 5,358,836 - -0.0 5,358,836 - -1.2 5,358,902 - -1.6 5,358,902 - -2.9 5,358,913 - +0.7 5,359,079 - Pf1N1B4_4850 0.14 +1.9 5,359,163 + Pf1N1B4_4850 0.20 +1.0 5,359,163 + Pf1N1B4_4850 0.20 +1.3 5,359,164 - Pf1N1B4_4850 0.20 -0.6 5,359,164 - +0.4 5,359,164 - +2.4 5,359,165 + Pf1N1B4_4850 0.20 +2.3 5,359,165 + Pf1N1B4_4850 0.20 +0.7 5,359,166 - Pf1N1B4_4850 0.20 +0.4 5,359,166 - +0.1 5,359,166 - Pf1N1B4_4850 0.20 +0.1 5,359,178 - Pf1N1B4_4850 0.21 -0.6 5,359,244 - -0.6 5,359,328 - Pf1N1B4_4850 0.32 -0.0 5,359,328 - Pf1N1B4_4850 0.32 +2.0 5,359,838 - Pf1N1B4_4850 0.71 -0.7 5,359,898 - Pf1N1B4_4850 0.75 +1.2 5,359,960 + Pf1N1B4_4850 0.80 -0.1 5,360,000 + Pf1N1B4_4850 0.83 -2.6 5,360,000 + Pf1N1B4_4850 0.83 -0.6 5,360,266 - +0.9 5,360,308 + +0.4 5,360,308 + -2.4 5,360,432 + +0.1 5,360,432 + -1.2 5,360,504 + -1.2 5,360,504 + -0.6 5,360,504 + -2.2 5,360,504 + -0.8 5,360,504 + -0.3 5,360,504 + -0.4 5,360,505 - -1.2 5,360,505 - -1.2 5,360,505 - -1.0 5,360,505 - -1.9 5,360,506 + +0.4 5,360,506 + -0.6 5,360,506 + +0.4 5,360,506 + +2.0 5,360,506 + +0.1 5,360,506 + +1.4 5,360,506 + -2.1 5,360,506 + -0.2 5,360,506 + +2.3 5,360,506 + -0.2 5,360,507 - +1.7 5,360,508 + -1.9 5,360,555 + +0.4 5,360,559 + +0.4 5,360,560 - +0.7 5,360,560 - -1.2 5,360,663 - Pf1N1B4_4851 0.12 -1.6 5,360,663 - Pf1N1B4_4851 0.12 -0.6 5,360,663 - Pf1N1B4_4851 0.12 -1.2 5,360,877 + Pf1N1B4_4851 0.37 -0.1 5,360,955 + Pf1N1B4_4851 0.46 -1.4
Or see this region's nucleotide sequence