Experiment: Bacitracin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4664 and Pf1N1B4_4665 overlap by 4 nucleotides Pf1N1B4_4665 and Pf1N1B4_4666 overlap by 4 nucleotides
Pf1N1B4_4664 - RND transporter, membrane fusion protein, at 5,140,620 to 5,141,921
_4664
Pf1N1B4_4665 - Acriflavin resistance plasma membrane protein, at 5,141,918 to 5,145,022
_4665
Pf1N1B4_4666 - Acriflavin resistance plasma membrane protein, at 5,145,019 to 5,146,605
_4666
Position (kb)
5141
5142
5143
5144
5145
5146 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5141.981 kb on - strand at 5142.446 kb on - strand, within Pf1N1B4_4665 at 5142.638 kb on + strand, within Pf1N1B4_4665 at 5142.639 kb on - strand, within Pf1N1B4_4665 at 5142.659 kb on + strand at 5142.887 kb on - strand, within Pf1N1B4_4665 at 5143.049 kb on + strand at 5143.050 kb on - strand, within Pf1N1B4_4665 at 5143.050 kb on - strand, within Pf1N1B4_4665 at 5143.554 kb on - strand, within Pf1N1B4_4665 at 5143.609 kb on + strand, within Pf1N1B4_4665 at 5143.715 kb on + strand, within Pf1N1B4_4665 at 5143.715 kb on + strand, within Pf1N1B4_4665 at 5143.716 kb on - strand, within Pf1N1B4_4665 at 5143.716 kb on - strand, within Pf1N1B4_4665 at 5144.049 kb on - strand, within Pf1N1B4_4665 at 5144.049 kb on - strand at 5144.049 kb on - strand, within Pf1N1B4_4665 at 5144.118 kb on - strand at 5144.153 kb on + strand, within Pf1N1B4_4665 at 5144.154 kb on - strand at 5144.154 kb on - strand, within Pf1N1B4_4665 at 5144.154 kb on - strand, within Pf1N1B4_4665 at 5144.525 kb on + strand, within Pf1N1B4_4665 at 5144.525 kb on + strand, within Pf1N1B4_4665 at 5144.525 kb on + strand, within Pf1N1B4_4665 at 5144.526 kb on - strand, within Pf1N1B4_4665 at 5144.526 kb on - strand, within Pf1N1B4_4665 at 5144.526 kb on - strand, within Pf1N1B4_4665 at 5144.526 kb on - strand, within Pf1N1B4_4665 at 5144.526 kb on - strand, within Pf1N1B4_4665 at 5144.555 kb on + strand, within Pf1N1B4_4665 at 5144.556 kb on - strand, within Pf1N1B4_4665 at 5144.765 kb on + strand at 5144.945 kb on - strand at 5144.948 kb on + strand at 5144.949 kb on - strand at 5144.949 kb on - strand at 5145.403 kb on + strand, within Pf1N1B4_4666 at 5145.403 kb on + strand, within Pf1N1B4_4666 at 5145.629 kb on + strand, within Pf1N1B4_4666 at 5145.630 kb on - strand, within Pf1N1B4_4666 at 5145.739 kb on + strand, within Pf1N1B4_4666 at 5145.826 kb on + strand, within Pf1N1B4_4666
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 2 mg/ml remove 5,141,981 - -2.5 5,142,446 - Pf1N1B4_4665 0.17 -1.7 5,142,638 + Pf1N1B4_4665 0.23 -2.2 5,142,639 - Pf1N1B4_4665 0.23 -0.6 5,142,659 + -0.6 5,142,887 - Pf1N1B4_4665 0.31 -2.6 5,143,049 + -0.2 5,143,050 - Pf1N1B4_4665 0.36 -3.4 5,143,050 - Pf1N1B4_4665 0.36 -0.6 5,143,554 - Pf1N1B4_4665 0.53 +1.4 5,143,609 + Pf1N1B4_4665 0.54 -0.6 5,143,715 + Pf1N1B4_4665 0.58 -1.9 5,143,715 + Pf1N1B4_4665 0.58 +0.1 5,143,716 - Pf1N1B4_4665 0.58 -2.6 5,143,716 - Pf1N1B4_4665 0.58 +0.4 5,144,049 - Pf1N1B4_4665 0.69 -0.6 5,144,049 - -0.6 5,144,049 - Pf1N1B4_4665 0.69 -0.2 5,144,118 - -1.2 5,144,153 + Pf1N1B4_4665 0.72 -0.6 5,144,154 - +0.8 5,144,154 - Pf1N1B4_4665 0.72 -1.9 5,144,154 - Pf1N1B4_4665 0.72 -1.2 5,144,525 + Pf1N1B4_4665 0.84 -1.9 5,144,525 + Pf1N1B4_4665 0.84 -1.2 5,144,525 + Pf1N1B4_4665 0.84 -1.8 5,144,526 - Pf1N1B4_4665 0.84 -1.6 5,144,526 - Pf1N1B4_4665 0.84 -1.6 5,144,526 - Pf1N1B4_4665 0.84 -1.2 5,144,526 - Pf1N1B4_4665 0.84 +2.0 5,144,526 - Pf1N1B4_4665 0.84 -0.6 5,144,555 + Pf1N1B4_4665 0.85 -0.6 5,144,556 - Pf1N1B4_4665 0.85 -0.8 5,144,765 + +0.4 5,144,945 - +1.4 5,144,948 + -0.6 5,144,949 - -1.2 5,144,949 - -1.9 5,145,403 + Pf1N1B4_4666 0.24 +0.0 5,145,403 + Pf1N1B4_4666 0.24 -0.6 5,145,629 + Pf1N1B4_4666 0.38 -1.2 5,145,630 - Pf1N1B4_4666 0.39 -1.2 5,145,739 + Pf1N1B4_4666 0.45 +0.0 5,145,826 + Pf1N1B4_4666 0.51 -2.7
Or see this region's nucleotide sequence