Experiment: Bacitracin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3938 and Pf1N1B4_3939 are separated by 79 nucleotides Pf1N1B4_3939 and Pf1N1B4_3940 are separated by 171 nucleotides Pf1N1B4_3940 and Pf1N1B4_3941 are separated by 320 nucleotides Pf1N1B4_3941 and Pf1N1B4_3942 are separated by 53 nucleotides
Pf1N1B4_3938 - Integral membrane protein TerC, at 4,374,721 to 4,375,470
_3938
Pf1N1B4_3939 - FIG00953489: hypothetical protein, at 4,375,550 to 4,375,720
_3939
Pf1N1B4_3940 - Glutamate synthase [NADPH] large chain (EC 1.4.1.13), at 4,375,892 to 4,376,620
_3940
Pf1N1B4_3941 - hypothetical protein, at 4,376,941 to 4,377,165
_3941
Pf1N1B4_3942 - Murein-DD-endopeptidase (EC 3.4.99.-), at 4,377,219 to 4,378,157
_3942
Position (kb)
4375
4376
4377 Strain fitness (log2 ratio)
-2
-1
0
1 at 4375.375 kb on + strand, within Pf1N1B4_3938 at 4375.375 kb on + strand, within Pf1N1B4_3938 at 4375.375 kb on + strand, within Pf1N1B4_3938 at 4375.376 kb on - strand, within Pf1N1B4_3938 at 4375.376 kb on - strand, within Pf1N1B4_3938 at 4375.376 kb on - strand, within Pf1N1B4_3938 at 4375.376 kb on - strand at 4375.478 kb on + strand at 4375.637 kb on - strand, within Pf1N1B4_3939 at 4375.640 kb on - strand at 4375.697 kb on + strand, within Pf1N1B4_3939 at 4375.697 kb on + strand, within Pf1N1B4_3939 at 4375.697 kb on + strand, within Pf1N1B4_3939 at 4375.698 kb on - strand, within Pf1N1B4_3939 at 4375.698 kb on - strand, within Pf1N1B4_3939 at 4375.698 kb on - strand at 4375.698 kb on - strand, within Pf1N1B4_3939 at 4375.698 kb on - strand at 4375.698 kb on - strand, within Pf1N1B4_3939 at 4375.698 kb on - strand at 4375.766 kb on + strand at 4375.790 kb on + strand at 4375.790 kb on + strand at 4375.790 kb on + strand at 4375.868 kb on + strand at 4375.891 kb on + strand at 4376.099 kb on + strand, within Pf1N1B4_3940 at 4376.672 kb on + strand at 4376.673 kb on - strand at 4376.854 kb on + strand at 4376.854 kb on + strand at 4376.855 kb on - strand at 4376.855 kb on - strand at 4376.895 kb on + strand at 4376.940 kb on + strand at 4376.940 kb on + strand at 4376.940 kb on + strand at 4376.940 kb on + strand at 4376.940 kb on + strand at 4376.940 kb on + strand at 4376.941 kb on - strand at 4376.941 kb on - strand at 4376.941 kb on - strand at 4376.941 kb on - strand at 4377.220 kb on + strand at 4377.221 kb on - strand at 4377.277 kb on + strand at 4377.277 kb on + strand at 4377.277 kb on + strand at 4377.277 kb on + strand at 4377.278 kb on - strand at 4377.278 kb on - strand at 4377.278 kb on - strand at 4377.278 kb on - strand at 4377.278 kb on - strand at 4377.278 kb on - strand at 4377.278 kb on - strand at 4377.362 kb on - strand at 4377.362 kb on - strand, within Pf1N1B4_3942 at 4377.362 kb on - strand, within Pf1N1B4_3942 at 4377.482 kb on + strand, within Pf1N1B4_3942 at 4377.526 kb on + strand, within Pf1N1B4_3942 at 4377.526 kb on + strand, within Pf1N1B4_3942 at 4377.526 kb on + strand, within Pf1N1B4_3942 at 4377.526 kb on + strand, within Pf1N1B4_3942 at 4377.527 kb on - strand, within Pf1N1B4_3942 at 4377.527 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 2 mg/ml remove 4,375,375 + Pf1N1B4_3938 0.87 -1.8 4,375,375 + Pf1N1B4_3938 0.87 -0.5 4,375,375 + Pf1N1B4_3938 0.87 +0.7 4,375,376 - Pf1N1B4_3938 0.87 +0.3 4,375,376 - Pf1N1B4_3938 0.87 -0.9 4,375,376 - Pf1N1B4_3938 0.87 -0.8 4,375,376 - -0.6 4,375,478 + +0.1 4,375,637 - Pf1N1B4_3939 0.51 -2.3 4,375,640 - -1.3 4,375,697 + Pf1N1B4_3939 0.86 -2.0 4,375,697 + Pf1N1B4_3939 0.86 +0.5 4,375,697 + Pf1N1B4_3939 0.86 -1.3 4,375,698 - Pf1N1B4_3939 0.87 -0.6 4,375,698 - Pf1N1B4_3939 0.87 -0.3 4,375,698 - -0.8 4,375,698 - Pf1N1B4_3939 0.87 +0.7 4,375,698 - -1.6 4,375,698 - Pf1N1B4_3939 0.87 -0.8 4,375,698 - -1.6 4,375,766 + +0.9 4,375,790 + -2.0 4,375,790 + -0.8 4,375,790 + -0.3 4,375,868 + +0.7 4,375,891 + -0.3 4,376,099 + Pf1N1B4_3940 0.28 +0.7 4,376,672 + +0.2 4,376,673 - +0.2 4,376,854 + -0.1 4,376,854 + -0.7 4,376,855 - -0.5 4,376,855 - +0.6 4,376,895 + -2.1 4,376,940 + +0.5 4,376,940 + -0.8 4,376,940 + -0.6 4,376,940 + +0.6 4,376,940 + -0.8 4,376,940 + +1.1 4,376,941 - +1.7 4,376,941 - -0.8 4,376,941 - +1.7 4,376,941 - +0.5 4,377,220 + -1.7 4,377,221 - -1.4 4,377,277 + -0.3 4,377,277 + +1.7 4,377,277 + -0.7 4,377,277 + +0.1 4,377,278 - +0.3 4,377,278 - -0.6 4,377,278 - -0.5 4,377,278 - -0.8 4,377,278 - -2.7 4,377,278 - +0.3 4,377,278 - +0.4 4,377,362 - +1.2 4,377,362 - Pf1N1B4_3942 0.15 -1.4 4,377,362 - Pf1N1B4_3942 0.15 -1.5 4,377,482 + Pf1N1B4_3942 0.28 -0.8 4,377,526 + Pf1N1B4_3942 0.33 -1.3 4,377,526 + Pf1N1B4_3942 0.33 +0.7 4,377,526 + Pf1N1B4_3942 0.33 -0.7 4,377,526 + Pf1N1B4_3942 0.33 +1.0 4,377,527 - Pf1N1B4_3942 0.33 +1.7 4,377,527 - -0.3
Or see this region's nucleotide sequence