Experiment: Bacitracin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3698 and Pf1N1B4_3699 are separated by 389 nucleotides Pf1N1B4_3699 and Pf1N1B4_3700 are separated by 30 nucleotides
Pf1N1B4_3698 - Phosphoribosylformylglycinamidine cyclo-ligase (EC 6.3.3.1), at 4,106,311 to 4,107,369
_3698
Pf1N1B4_3699 - FIG00954480: hypothetical protein, at 4,107,759 to 4,108,817
_3699
Pf1N1B4_3700 - Putative permease often clustered with de novo purine synthesis, at 4,108,848 to 4,109,921
_3700
Position (kb)
4107
4108
4109 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4106.925 kb on - strand at 4107.604 kb on + strand at 4107.604 kb on + strand at 4107.604 kb on + strand at 4107.604 kb on + strand at 4107.604 kb on + strand at 4107.605 kb on - strand at 4107.605 kb on - strand at 4107.605 kb on - strand at 4107.606 kb on + strand at 4107.606 kb on + strand at 4107.607 kb on - strand at 4107.634 kb on + strand at 4108.397 kb on - strand, within Pf1N1B4_3699 at 4108.397 kb on - strand, within Pf1N1B4_3699 at 4108.826 kb on + strand at 4108.827 kb on - strand at 4108.827 kb on - strand at 4108.827 kb on - strand at 4108.828 kb on + strand at 4108.828 kb on + strand at 4108.828 kb on + strand at 4108.829 kb on - strand at 4108.830 kb on + strand at 4108.831 kb on - strand at 4108.831 kb on - strand at 4108.845 kb on + strand at 4108.845 kb on + strand at 4108.908 kb on + strand at 4108.959 kb on + strand, within Pf1N1B4_3700 at 4108.960 kb on - strand, within Pf1N1B4_3700 at 4108.960 kb on - strand, within Pf1N1B4_3700 at 4109.110 kb on - strand, within Pf1N1B4_3700 at 4109.110 kb on - strand, within Pf1N1B4_3700 at 4109.110 kb on - strand, within Pf1N1B4_3700 at 4109.505 kb on + strand, within Pf1N1B4_3700 at 4109.506 kb on - strand, within Pf1N1B4_3700 at 4109.506 kb on - strand at 4109.586 kb on + strand, within Pf1N1B4_3700 at 4109.587 kb on - strand, within Pf1N1B4_3700 at 4109.587 kb on - strand, within Pf1N1B4_3700 at 4109.656 kb on - strand, within Pf1N1B4_3700
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 2 mg/ml remove 4,106,925 - -0.2 4,107,604 + +0.2 4,107,604 + +0.5 4,107,604 + -0.0 4,107,604 + +1.2 4,107,604 + -2.3 4,107,605 - -1.4 4,107,605 - -0.2 4,107,605 - -0.8 4,107,606 + -0.2 4,107,606 + -0.2 4,107,607 - -0.2 4,107,634 + -2.6 4,108,397 - Pf1N1B4_3699 0.60 -0.2 4,108,397 - Pf1N1B4_3699 0.60 -0.6 4,108,826 + -0.8 4,108,827 - +1.4 4,108,827 - +0.4 4,108,827 - -2.8 4,108,828 + +1.8 4,108,828 + -0.4 4,108,828 + -1.5 4,108,829 - -0.5 4,108,830 + -0.7 4,108,831 - -0.3 4,108,831 - -1.0 4,108,845 + -0.3 4,108,845 + -2.0 4,108,908 + -0.6 4,108,959 + Pf1N1B4_3700 0.10 -1.0 4,108,960 - Pf1N1B4_3700 0.10 +0.6 4,108,960 - Pf1N1B4_3700 0.10 -0.4 4,109,110 - Pf1N1B4_3700 0.24 -1.8 4,109,110 - Pf1N1B4_3700 0.24 -1.0 4,109,110 - Pf1N1B4_3700 0.24 -0.5 4,109,505 + Pf1N1B4_3700 0.61 -1.3 4,109,506 - Pf1N1B4_3700 0.61 -1.0 4,109,506 - -0.2 4,109,586 + Pf1N1B4_3700 0.69 +1.8 4,109,587 - Pf1N1B4_3700 0.69 -0.4 4,109,587 - Pf1N1B4_3700 0.69 +0.6 4,109,656 - Pf1N1B4_3700 0.75 -1.2
Or see this region's nucleotide sequence